Prdm4 (NM_001302887) Mouse Untagged Clone

CAT#: MC228469

Prdm4 (untagged) - Mouse PR domain containing 4 (Prdm4), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prdm4
• Synonyms: 1700031E19Rik; 2810470D21Rik; AW552272; SC; SC-; SC-1; SC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228469 representing NM_001302887
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAATGTTTCTAGGGTTACCAGCCCAATCTCCACAGATGGAATGGCAGAGGAGCTTACAATGGACG
  GTGTTACAGGAGAGCATCCCCAAATCCCAAATGGCTCCAGAAGTCATGAGCCTCTCTCTGTGGATTCTGT
  GAGCAACAGCCTTACAGCAGAAGCTGTAGGACATGGTGGTGTGATACCCATTCACGGGAATGGTCTGGAG
  CTTCCTGTGGTCATGGAAACTGACCACATTGCAAACCGGGTCAATGGGATGTCTGACAGTACCCTCAGTG
  ACTCCATCCACACCGTGGCCATGAGCACCAACTCTGTAAGCGTGGCACTCTCTACCTCACACAACCTGGC
  CTCCCTAGAGTCTGTTTCCCTCCATGAAGTTGGCCTTAGCCTAGAGCCTGTGGCTGTCTCTTCTATCACG
  CAGGAGGTTGCCATGGGGACAGGTCATGTAGATGTGTCCTCAGACAGCTTGTCTTTTGTACCATCTTCAC
  TGCAAATGGAAGACTCCAATTCAAACAAGGAAAACATGGCAACCTTGTTTACAATTTGGTGCACTCTTTG
  TGACCGAGCCTACCCCTCAGATTGCCCTGATCATGGACCAGTGACTTTTGTTCCTGACACACCAATAGAG
  AGCAGAGCAAGACTATCCCTCCCGAAGCAGCTTGTTCTCCGCCAGTCCATCGTGGGAACAGATGTTGTTG
  GTGTCCTTCCATTGATAGGTGTATGGACTGCAGAAACCATTCCTGTGCGGACTTGCTTCGGACCTCTAAT
  TGGTCAGCAGAGTCACTCCTTGGAAGTAGCAGAGTGGACAGACAAGGCAGTTAACCATGTCTGGAAGATA
  TACCACACTGGTGTCCTGGAGTTCTGTATCATTACAACCGATGAGAATGAGTGTAATTGGATGATGTTTG
  TGCGCAAAGCCAGGAACCGTGAAGAACAGAATTTGGTGGCCTATCCCCATGATGGGAAAATCTATTTCTG
  TACCTCACAAGACATCCCCCCTGAAAGTGAGCTGCTTTTCTATTACAGCCGGAATTACGCTCAACAGATA
  GGTGTTCCTGAACACCCAGATGTGCACCTCTGTAACTGTGGCAAGGAGTGCAGTTCCTATTCAGAGTTCA
  AAGCTCATCTGACCAGCCATATCCACAATCATCTCCCTAGCCAGGGCCACAGCAGCAGCCATGGGCCAAG
  CCACAGCAAGGAAAGGAAGTGGAAGTGTTCAATGTGCCCCCAGGCTTTTATCTCGCCTTCCAAACTCCAC
  GTTCACTTTATGGGCCACATGGGTATGAAGCCCCACAAGTGTGATTTTTGTAGCAAGGCTTTTAGTGATC
  CAAGCAACCTACGGACACACCTCAAAATACATACAGGTCAGAAAAATTATAGGTGTACTTTGTGTGACAA
  GTCTTTCACGCAGAAGGCTCACCTGGAGTCTCACATGGTCATCCACACGGGTGAGAAGAATCTCAAGTGT
  GATTACTGTGACAAGCTGTTCATGCGGAGACAGGACCTCAAGCAGCACGTGCTCATCCACACGCAAGAAC
  GCCAGATCAAGTGTCCGAAGTGTGATAAACTGTTCTTGAGAACAAACCACTTGAAGAAGCATCTCAATTC
  TCACGAAGGAAAACGAGATTATGTCTGCGAAAAATGTACAAAGGCTTATCTAACCAAGTACCATCTCACT
  CGCCACCTGAAGACCTGCAAAGAGCCCAGCTCCAGCTCCTCTGCACAGGAGGAAGAGGATGATGAGTCGG
  AGGAAGAAGATCTCGCAGACTCCATGAGGACAGAGGACTGCAGGATGGGCAGTGCTGTTTACTCAACAGA
  CGAGTCTCTCTCCGCACATAAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302887
• Insert Size: 1845 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302887.1, NP_001289816.1
• RefSeq Size: 3861 bp
• RefSeq ORF: 1845 bp
• Locus ID: 72843
• Cytogenetics: 10 C1
• Gene Summary: This gene encodes a member of the PR/SET family of zinc finger proteins. This protein has been shown to bind DNA in a sequence-specific manner and has been implicated in neural stem cell proliferation and differentiation. Pseudogenes have been identified on chromosomes 14 and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
  Transcript Variant: This variant (3) contains an alternate exon in the 5' region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus than isoform 1.