Elf1 (NM_001286411) Mouse Untagged Clone

CAT#: MC228463

Elf1 (untagged) - Mouse E74-like factor 1 (Elf1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Elf1
• Synonyms: Elf-1; mElf-1; p70; Sts1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228463 representing NM_001286411
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTGTTGTCCAACAGAACGACCTAGTGTTTGAATTTGCAAGTAACGGCATGGAGGACGAGCAGC
  AGCTTGGTGATCCTGCTATATTTCCTGCTGTTATTGTGGAACATGTTCCTGGTGCTGATATTCTCAATAG
  TTATGCTGGTCTAGCTTGTGTGGAAGAGCCTAATGACATGATTACTGAGAGCTCATTGGATGTTGCTGAA
  GAAGAAATCATAGACGATGACGATGACGACATCACCCTTACAGTTGAGGCTTCTTGTCATAATGGAGATG
  AAACAATTGAGACCATTGAGGCTGCCGAGGCACTCCTCAATATAGATTCTCCCAGCCCTCCTGTACTGGA
  TGAAAAGCAAATCAATAATAATATATTTAGCTCCTCTGAAGATGACATCGTTGCCCCAATCACCCATGTG
  TCCGTCACATTAGATGGGATTCCTGAAGTGATGGAAACTCAGCAAGTGCAAGAGACAAATGCAGACTCCC
  CAGGAGCCTCCTCCCCAGAGCAGCGCAAGAGAAAGAAAGGAAGGAAAACAAAACCACCACGGCCAGATTC
  CCCGACGACTACACCAAATATCTCTGTGAAAAAGAAAAATAAAGATGGGAAGGGAAACACAATTTACCTT
  TGGGAGTTTTTGCTGGCCCTGCTTCAGGACAAAGCTACCTGTCCTAAGTACATCAAGTGGACGCAGCGAG
  AAAAGGGCATTTTTAAGCTGGTTGATTCTAAAGCCGTGTCTAGATTGTGGGGGAAGCACAAAAACAAACC
  TGACATGAACTACGAGACCATGGGGAGAGCACTCAGGTACTATTACCAAAGAGGTATTCTTGCAAAAGTG
  GAAGGTCAGCGCTTGGTGTATCAGTTTAAAGAAATGCCAAAAGATCTTATCTACATAGATGATGAGGATC
  CAAGTTCCAGCATAGAGTCTTCGGATCAGTCCTTATCGTCAACAACCGCATCAAGTCGGAATCAAGCAAA
  CCGATCAAGAGTATCTTCAAGTCCAGGAATTAAAGGAGGGGCCGCTACAATTCTAAAGCCCGGGAATTCT
  AAAGCTGCAAACCCCAAAGATCCGGTGGAAGTTGGACAACCTTCAGAAGTTCTGAGGACAGTGCAACCCT
  CACAGGCTCCCTATCCTACCCAGCTCTTCCGAACTGTTCATGTAGTACAGCCAGTACAAGCTGTCCCAGA
  GGAAGCAACCATAGCCAGCACCATGCAGGAGGAAGCAGCAAATTCTTCAGTTCCAAGTATTAGGACTATA
  CAGGCTTCAACCCAGGTTCCAGTAGTGGTGTCTCCTGGCAACCAACAGTTACATACAGTAACAGTGCCAC
  TCACAACGGTTATAGCCAGCATAGACCCATCATCAGGTGCTGGGTCTCAGAAATTCATTTTACAAACCAT
  TCCATCGTCACAGCCCATGACAGTACTGAAAGAAAATGTCATGCTACAGTCACAGAAGCCAGGCTCTCCT
  TCAATTGTCCTCAGCCCCACCCAAGTACAGCAGGTCCTCACAAGCAATGTTCAGTCCATTTGCAATGGAG
  CGGGCAGTGTGGCATCCGCACCATCGTTCAGCGCGACGACACCTGTGGTGACTTTCTCTCGGAGTTCACA
  GCTGGTCGCACACCCACCGGGCACTGTGATCACTTCTGTTATCAAAGCTCAGGAAACAAAAACTCTTAAG
  CAAGAGGTGGAGAAAAAGGCCGAAGATGATTTGAATGAAGATGCTGAGAAAAGTGCCCAGCAGCCCCAGC
  CTTATGTGATGGTGTTATCCAGTTCAAACGGGTTTTCCTCTCAGGTAGCCGTCAAACAGAATGAACTGCT
  AGAGCCCAACTCTTTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286411
• Insert Size: 1839 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286411.1, NP_001273340.1
• RefSeq Size: 3885 bp
• RefSeq ORF: 1839 bp
• Locus ID: 13709
• UniProt ID: Q60775
• Cytogenetics: 14 D3
• Gene Summary: Transcription factor that activates the LYN and BLK promoters.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1).