Podn (NM_001285956) Mouse Untagged Clone

CAT#: MC228460

Podn (untagged) - Mouse podocan (Podn), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Podn
• Synonyms: 9430070G18; Pcan; SLRR5A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228460 representing NM_001285956
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGCAGCAGGGGCCTGCCACTCCTACTGCTGGTGCTTCAGCTCTTCCTGGGCCCTGTGCTGCCTG
  TGAGGGCACCTGTGTTTGGCCGAAGTGACACCCCCACCCTGAGCCCCGAGGAGAATGAATTTGTGGAGGA
  AGAGAATCAGCCAGTGCTGGTTCTGAGCTCCGAGGAGCCAGAGCCTGGCCCAGCCACTGTCGACTGTCCC
  CGAGATTGTGCCTGTTCCCAGGAAGGTGTAGTGGACTGTGGTGGCATTGACCTGCGTGAGTTTCCAGGCG
  ACCTGCCCGAGCACACCAACCATCTCTCCTTGCAGAACAACCAGCTGGAGAAGATCTACCCCGAGGAGCT
  GTCCCGGCTGCAGCGGCTGGAGACGCTGAACCTGCAGAACAACCGCCTGACATCCCGAGGGCTCCCAGAG
  GAGGCATTTGAGCATCTTACTAGCCTCAATTACCTGTACCTGGCCAACAACAAGCTGACACTGGCACCCC
  GATTCCTGCCAAACGCCCTGATCAGTGTGGACTTTGCTGCCAATTATCTCACTAAGATCTATGGACTCAC
  CTTTGGCCAAAAGCCAAATCTGAGGTCTGTGTACCTGCATAACAACAAGCTAGCAGATGCCGGGCTGCCG
  GACCACATGTTCAATGGCTCCAGCAACGTCGAGATCCTAATCCTGTCCAGCAACTTCCTGCGCCACGTGC
  CCAAGCACCTGCCACCCGCTCTGTACAAGCTGCACCTCAAGAACAATAAGCTAGAGAAGATCCCCCCTGG
  GGCCTTCAGTGAGCTGAGCAACCTACGGGAACTCTACCTGCAGAACAACTACCTGACCGACGAGGGTCTG
  GACAACGAGACCTTCTGGAAGCTGTCCAGCCTGGAGTACCTGGACTTGTCCAGCAACAACCTGTCGAGGG
  TCCCAGCGGGTCTTCCCCGCAGCCTGGTCCTGCTGCACCTGGAGAAAAATGCCATCCAGAGCGTAGAAGC
  TGATGTGCTGACACCCATCCGCAACCTGGAGTACCTGCTGCTACATAGCAACCAGCTGCAGGCCAAGGGT
  ATCCACCCACTGGCCTTCCAGGGCCTCAAGAAGCTCCACACAGTGCATCTATACAACAACGCGCTGGAAC
  GTGTGCCCAGCGGCCTGCCCCGCCGAGTGCGCACCCTCATGATCCTGCACAACCAGATTACAGGCATAGG
  CCGTGAGGACTTCGCTACCACCTACTTCCTGGAAGAGCTCAACCTCAGCTACAACCGCATCACCAGCCCA
  CAGATGCACCGAGATGCCTTCCGCAAGCTACGCCTGCTGCGTTCACTTGACTTGTCTGGCAACCGTCTGC
  AAACACTGCCTCCAGGCCTGCCGAAAAACGTACACGTGCTCAAGGTCAAGCGGAATGAGCTGGCTGCCCT
  GGCACGTGGGGCACTAGCTGGCATGGCCCAGCTTCGGGAACTCTACCTCACAGGCAACCGACTGCGAAGC
  CGGGCCCTGGGACCCCGTGCCTGGGTGGACCTTGCTGGTCTGCAGCTGCTGGACATCGCTGGGAATCAGC
  TCACAGAGGTCCCTGAGGGGCTCCCCCCATCTCTGGAGTATCTGTACCTGCAGAATAACAAGATTAGTGC
  CGTTCCTGCCAACGCCTTTGACTCCACTCCCAACCTTAAGGGGATCTTTCTCAGGTTCAACAAGCTGGCT
  GTGGGCTCCGTGGTGGAAAGCGCCTTCCGGAGGCTGAAACACCTGCAGGTCTTGGACATTGAAGGCAACT
  TTGAGTTTGGTAATGGTTCCAAGGACAAAGATGAGGAAGAGGAAGAAGAGGAGGAAGAGGAAGATGAGGA
  AGAGGAAACTAGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285956
• Insert Size: 1836 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285956.1, NP_001272885.1
• RefSeq Size: 3262 bp
• RefSeq ORF: 1836 bp
• Locus ID: 242608
• UniProt ID: Q7TQ62
• Cytogenetics: 4 C7
• Gene Summary: Negatively regulates cell proliferation and cell migration, especially in smooth muscle cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 all encode the same protein.