Adam19 (NM_001291891) Mouse Untagged Clone

CAT#: MC228441

Adam19 (untagged) - Mouse a disintegrin and metallopeptidase domain 19 (meltrin beta) (Adam19), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adam19
• Synonyms: AL024287; MADDAM; Mlt; Mltnb; MTLNB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228441 representing NM_001291891
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGTGCTCCGTGTACCAGTCTGGAGGAGTTAGCATGGACCACTCCGAGAATGCCATTGGTGTAG
  CCTCCACTGTGGCCCATGAGATTGGCCACAACTTTGGCATGAGCCATGATTCTGCACACTGCTGTTCTGC
  CAGTGCAGCCGATGGCGGCTGCATCATGGCCGCCGCCACCGGGCACCCTTTCCCCAAAGTGTTCAGTTGG
  TGTAACAGGAAGGAGCTGGACAGGTATCTGCAGACAGGAGGAGGGATGTGTCTCTCCAACATGCCGGACA
  CTAGGACGCTGTATGGAGGCCGGAGGTGTGGCAACGGGTACCTGGAAGACGGTGAAGAATGTGACTGTGG
  AGAAGAGGAGGAATGTAAGAACCCTTGCTGCAATGCCTCCAACTGCACTCTGAAGGAAGGGGCAGAGTGT
  GCCCATGGTTCCTGCTGCCACCAGTGCAAGCTGGTGGCTCCTGGAACCCAGTGTCGGGAGCAGGTTCGGC
  AATGTGACCTCCCCGAGTTCTGCACCGGCAAGTCTCCCCACTGCCCCACCAACTATTATCAGATGGATGG
  CACCCCCTGCGAGGGTGGCCAGGCCTACTGCTACAACGGCATGTGCCTCACTTACCAGGAACAGTGCCAG
  CAGCTGTGGGGACCTGGAGCCCGGCCTGCCCTCGATCTTTGCTTTGAGAGGGTGAATGCTGCTGGTGACA
  CCTATGGAAACTGTGGCAAGGGCTTGAATGGCCAATACAGGAAGTGCAGTCCCAGGGATGCCAAGTGTGG
  GAAGATTCAGTGCCAGAGCACCCAGGCCCGGCCCCTGGAATCCAACGCAGTATCTATTGACACCACCATC
  ACCTTGAACGGGAGGCGGATCCACTGTCGGGGCACCCACGTCTACCGGGGTCCTGAGGAGGAGGAAGGGG
  AAGGTGACATGCTGGACCCAGGGCTGGTGATGACTGGAACCAAGTGTGGCCACAACCATATTTGCTTCGA
  GGGGCAGTGCAGGAACACCTCCTTCTTTGAGACGGAAGGCTGTGGGAAAAAGTGCAATGGCCACGGGGTC
  TGCAACAACAACAAGAACTGTCATTGCTTCCCTGGCTGGTCTCCACCTTTCTGTAACACCCCGGGAGATG
  GTGGCAGCGTCGACAGTGGTCCTTTGCCCCCTAAGAGTGTGGGTCCCGTGATCGCTGGGGTGTTTTCAGC
  TCTCTTCGTGTTGGCAGTTCTGGTGCTACTGTGTCACTGCTACAGACAGAGCCACAAACTGGGCAAACCC
  TCGGCTCTCCCTTTCAAGCTGCGGCATCAGTTCAGTTGTCCCTTCAGGGTATCTCAGAGTGGTGGAACTG
  GCCATGCCAACCCAACTTTCAAGTTGCAGACCCCCCAGGGCAAGCGAAAGGTGACTAACACCCCTGAATC
  CCTCCGGAAGCCGTCCCACCCCCCTCCCCGGCCCCCTCCAGACTACCTGCGCGTTGAATCGCCACCTGCA
  CCATTGCCGGCACATCTGAACAGGGCTGCTGGGAGCTCCCCAGAAGCTGGGGCTCGAATAGAAAGAAAGG
  AGTCAGCCAGGAGGCCTCCCCCAAGCCGACCCATGCCCCCTGCACCTAACTGCCTACTGTCCCAGGACTT
  CTCCAGGCCTCGACCACCTCAGAAGGCACTCCCAGCCAATCCGGTGCCAGGCCAAAGGACCGGTCCCAGG
  TCAGGAGGCACCTCCCTGCTTCAGCCCCCTACTTCTGGTCCTCAGCCCCCCAGGCCTCCAGCAGTGCCTG
  TTCCAAAGCTACCCGAGTACCGATCACAGAGGGTTGGAGCAATAATTAGCTCCAAGATCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291891
• Insert Size: 1812 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291891.1, NP_001278820.1
• RefSeq Size: 6421 bp
• RefSeq ORF: 1812 bp
• Locus ID: 11492
• Cytogenetics: 11 27.54 cM
• Gene Summary: This gene encodes a cell surface glycoprotein and member of the ADAM (a disintegrin and metalloproteinase) family of endopeptidases. The encoded protein may play a role in the ectodomain shedding of neuregulin proteins. Homozygous knockout mice for this gene exhibit heart development defects and perinatal lethality. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that undergoes proteolytic processing to generate a mature protein product. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (3) uses an alternate internal splice site, compared to variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG to encode an isoform (3) that has a shorter N-terminus, compared to isoform 1.