Slfn4 (NM_001302559) Mouse Untagged Clone

CAT#: MC228433

Slfn4 (untagged) - Mouse schlafen 4 (Slfn4), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slfn4
• Synonyms: AI450778
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228433 representing NM_001302559
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGAAACACCCCACAGCTGGAGAAAGATGTGAATCCACAGACATCCATAGAGCCCTCTGTTCAAG
  TCAAGTGTCCAGGGGATATTCTTCAGGGAGGACGGCATGCCAAGCGAGGAAGATGGATGGCGTCGTGGAA
  AGGATTTCATCTGGGAAGGAGAGACAGTGACTGCAAAATGAACATCACTGTCGATCAGGACACGGACTAC
  GCTGAACTGGTTCTGTCTGTAGGAGAAATCACACTCGGAGAGAAGACTAGGGATTCAATGAAAGATAGCC
  AACTGAGAAGAAAAGAAGCCAAAAGTATATTACAGGCTGTGTGCACCCTGCTGAATTCTGGAGGGGGTGT
  GGTCAAGGCTCACATTAAAAATCAAAACTACAGCTTCACCAGAGATGGAATGGGACTGGATTTGGTAAAT
  CCCTTGCCTGGTATCCTGCACCTTCCTCATGACTATCTAGACTTCATGCAGTACAACGACTACTTTTTCG
  TTTTTGTGAAACCATGGAAGCCGAATCAGAGAGGTCCGGGGATCGCCACCTGGAAAACCAACTTGTACAA
  GAGAATCTTCTCATTCTCAGTTGAACTGAAAGCAGCGGATGCAGTGCAGTTCCTCAAATCCAGACCAAGT
  TCCCATGGAAAAACAGTCTGTAATGAAACACTAAATGAATGTCTTTCCTTATTTAACAGAGACTGGCTTG
  CCTATGAGGAGACATTCTGTTTCACTAAATCCACACATGCTGAAGTAAAATTGACTCCTAAGGAAAAGAT
  TTCTCCTAAGGAAAAGATTTTAGAGCTCCTCCCTCAAACTGTTTCTGCATTTGCAAACACTGATGGGGGA
  TATTTGTTCATTGGCTTGGATGGCAAAACGCAGCAAATTATTGGTTTTGAAGCAGAGAAGAGCGATCTCG
  TGCTTCTAGAGAGTGAAATAGAAAAGTGCATCCAACAGCTGCCTGTCACTCACTTCTGTGAGGAGAAGGA
  GAAGATCAAGTACACGTGCAAATTCATCGAAGTGCACAAATCCGGAGCTGTGTGTGCATATGTGTGCGCG
  ATCAGAGTGGAGAAGTTCTGCTGTGCAGTGTTCGCTGCAGAGCCTGAGTCCTGGCACGTGGAAGGCAGCT
  GTGTGAAGAGGTTTACCACAGAGGAATGGGTGAAGCGCCAGATGGATGCCACTGCAGTGATGCCTGGAAA
  GGTGATATGCTCTCCAGAAGCCCTCTGCATGAAACCATTCTCACAACATGAAGGCTATGAGCAGTTAGTC
  CGGACAGAATTGGGCTCCCTGCGTAAAGGAACACTGGTTGTCTCCAAGAGCTGGGCTTTGGATCTGGGCT
  TGCAAGAGAAGCAGGAAGTTATCTGGGATGTGCTTCATATTTCCCAGGGCAGTCTCCTGACCCTGCATGT
  CTTTGTCCAGGGAGATGAGAACCTGGAAGGCAACAGCAGTCTTCTTGGGAAACTAGGCGCCGAGTTAAAG
  GGCTATTATAAACAAATTGCCCTAACATTAAAGCAGACGTTGGTAAACCATTGTGGTTACACTGCGAAAA
  TAGGTATTATAGTAAAGATAACATACTTGGGTCATAAGACAATGTGTCTTTATGACTCAAGCGCGAAAAT
  ATGTTACCCCCAAATATATTATCTGACTACCAAGGCAGTAAAGGACTTAGAAAAAGCTCTCGCTGAAATC
  TTAGGAAGTTATGAGTCTTTCTACAGTTTACCCAGGAGAAACTGGGATTCTTTTATGTCTGCGTTTTTAA
  ATGTGGGCAGTTATATAGTTTACTTTTCGAGAAATGTCTTAACTCATATTCGAATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302559
• Insert Size: 1809 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302559.1, NP_001289488.1
• RefSeq Size: 3878 bp
• RefSeq ORF: 1809 bp
• Locus ID: 20558
• Cytogenetics: 11 C
• Gene Summary: The protein encoded by this gene belongs to the Schlafen family. All members of this family contain a Schlafen box domain that lies near an AAA domain. This protein belongs to the group 2 subset of Schlafen proteins, which are defined by a molecular weight between 58 kDa and 68 kDa and by the presence of a SWADL domain that contains the sequence Ser-Trp-Ala-Asp-Leu. In malignant melanoma cells, gene expression is up-regulated in response to interferon alpha. In bone marrow-derived macrophages, expression of this gene is induced during activation by Toll-like receptor agonists and repressed during macrophage colony-stimulating factor-mediated differentiation. Myelopoiesis is disrupted by constitutive overexpression in myeloid-lineage cells. A pseudogene of this gene is found on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.