Ly9 (NM_001277968) Mouse Untagged Clone

CAT#: MC228243

Ly9 (untagged) - Mouse lymphocyte antigen 9 (Ly9), transcript variant 2


  "NM_001277968" in other vectors (1)

Reconstitution Protocol

USD 558.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Ly9
Synonyms AI893573; CD229; Lgp100; Ly-9; SLAMF3; T100
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228243 representing NM_001277968
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGATCTAAAGAGATATTGGTGTGACTGGGCTCTAGGTCCACTCTCTGAAAATCCAAGAATGAGTC
AGCAGCAGATATTTTCTCCCATTCTTTGGATTCCTCTCCTCTTCCTACTCATGGAGAAGCTCCAGAAGCC
TCAGATCATCGTGGAATCTGTGACCCCATCCGATACTGATTCCTGCACCTTCACCCTAATCTGCACTGTA
AAGGGGACAAAGGACAGTGTCCAGTACAGCTGGACCCGAGAGGACACCCATTTAAATACATACGATGGAA
GCCACACCCTCAGGGTTTCCCAGAGTGTCTGTGACCCCGACCTACCCTATACCTGCAAAGCCTGGAATCC
AGTCAGCCAAAACAGCTCCCAACCTGTCCGCATCTGGCAATTCTGCACAGGAGCCTCCAGAAGAAAAACA
GCAGCGGGGAAGACTGTGGTAGGAATCCTGGGAGAGCCAGTGACCCTGCCCTTGGAATTTCGGGCCACTC
GGGCCACAAAGAATGTTGTCTGGGTGTTTAACACGTCGGTTATCAGCCAAGAACGGAGAGGAGCAGCAAC
AGCGGATTCTCGCCGTAAGCCCAAAGGTTCTGAAGAACGGAGGGTGAGGACCTCTGACCAGGACCAATCC
CTGAAGATCAGCCAGCTGAAGATGGAGGACGCGGGCCCCTACCATGCCTATGTGTGCTCAGAGGCCTCCC
GAGACCCCAGTGTGAGACATTTCACCTTGCTTGTCTACAAGAGACTGGAGAAGCCCAGTGTCACCAAGAG
TCCTGTGCACATGATGAACGGAATCTGCGAGGTTGTCCTGACCTGTTCAGTGGACGGTGGTGGAAACAAT
GTGACATACACATGGATGCCTCTACAAAACAAAGCTGTCATGTCCCAAGGGAAGTCGCACCTCAACGTCT
CCTGGGAAAGTGGTGAACACCTGCCCAACTTCACATGCACAGCCCATAACCCTGTCAGCAACAGCTCCAG
CCAGTTTTCTTCTGGGACCATCTGTTCAGGCCCTGAGAGAAACAAGAGGTTTTGGCTCCTGCTCCTCCTG
GTTTTGCTCTTGTTGATGCTCATTGGCGGTTACTTCATTTTGAGGAAAAAGAAGCAGTGTTCGTCTTTGG
CCACCAGGTACAGACAAGCGGAGGTCCCAGCTGAAATACCAGAAACCCCAACTGGCCATGGACAATTTTC
TGTGCTCTCCCAACGGTATGAGAAACTAGACATGTCTGCTAAGACCACCAGGCATCAGCCTACACCCACC
TCAGATACCAGCTCTGAGAGCAGCGCAACAACAGAAGAGGATGACGAAAAGACCAGAATGCACAGCACTG
CTAATAGTAGAAATCAGGTGTATGACTTGGTCACCCATCAGGACATTGCACATGCCTTGGCCTATGAGGG
GCAAGTAGAATATGAAGCAATCACTCCATATGATAAAGTGGATGGGTCTATGGATGAAGAGGACATGGCA
TATATACAAGTGTCCCTGAATGTGCAGGGAGAGACCCCACTTCCTCAGAAGAAAGAAGACTCAAATACAA
TCTACTGCTCTGTGCAGAAGCCTAAAAAGACGGCACAAACACCACAGCAAGATGCTGAGTCTCCTGAAAC
CCCTACCTATGAAAATTTCACCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001277968
Insert Size 1635 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001277968.1, NP_001264897.1
RefSeq Size 2169 bp
RefSeq ORF 1635 bp
Locus ID 17085
UniProt ID Q01965
Cytogenetics 1 79.54 cM
Gene Summary Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2 (PubMed:19648922). May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. Promotes T-cell differentiation into a helper T-cell Th17 phenotype leading to increased IL-17 secretion; the costimulatory activity requires SH2D1A. Promotes recruitment of RORC to the IL-17 promoter (By similarity). May be involved in the maintenance of peripheral cell tolerance by serving as a negative regulator of the immune response. May disable autoantibody responses and inhibit IFN-gamma secretion by CD4(+) T-cells (PubMed:23914190). May negatively regulate the size of thymic innate CD8(+) T-cells and the development of invariant natural killer T (iNKT) cells (PubMed:23225888). Can promote natural killer (NK) cell activation (PubMed:19648922).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.