Fam214b (NM_001253354) Mouse Untagged Clone

CAT#: MC228223

Fam214b (untagged) - Mouse family with sequence similarity 214, member B (Fam214b), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fam214b
• Synonyms: B230312A22Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228223 representing NM_001253354
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCACGTGCAGGCGGAGCTGTCTCCATCCTCAGAGCCAGAGGCTGGCCCTTCACAGCCTCCAGTCA
  GACAGGGGACCCTCCAGGGTGGCCTGCTCATGGGCTACAGCCCAGCAGGGGGGGCGACATCCCCCGGGGT
  CTACCAGGTATCCATCTTTTCCCCTTCAGCTGGTGCCTCCGAGCCTCCTAGGGCTCTGAAGCGGCCGGCC
  CCTCCCACCGAGGGTCCCCGGGAGCTGAAGAGAGGCCCTGGGCTGGGGGCCAGAGAGGGACTACCCCCTG
  AAGAACCATCTACTGTGGGACTCTTGAGCCCAGAGGGACTGGGTCTGGGACTGGGTGTGGCTAGCCAGCA
  TTTCTCCCACCATGGCCTCTGTGTTGTGGAGCACGGAGGTAATACCACCTCACCTTGGACTTCAGGGACC
  CAGAGTACACCCTGGCTCTCATCAAATGCTTCCTTCAATACTTTGCACACCAGAGACTGGGCTTTCCCAG
  ATCAAGGGGGACAGGGGTGCCTGGGGGAGACTCCAGGGCCAGCCCCTTCAGGCCAGCTGCACACACTTGA
  CACTGATTTGCACAATCTTGCACAAATAGGGGGTAAGAGCCCAGTGGCTAGGGTGGGCAACGGGAGCAAC
  CCCTGGCCTAGGGAGTCCCATGGAACTGCCAATGGGCACAGTCCCGAGCACACACCCCCTGGCCCTGGAC
  CTCCTGGCCCCTGCCCCACCAAGCGAAGGCTGCTGCCTGCTGGAGAAACCCTTGATGTCAGCTCTGAGGA
  TGAGGGGCCAGCCCCTCGGAGGCGCCGGGGAACCCTGGGCTGCCCTCTTGCTGCCAATAGTTCTGATGCC
  AAAGCCACACCCTTCTGGAGCCACCTGCTGCCTGGGCCCAAGGAGCCTGTTTTGGACCCAACAGACTGCA
  GTCCCATGGGGCGGAGGCTGAAAGGCGCCCGTCGCCTGAAGCTAAGCTCCCTTCGAACTCTCCGGAAGGG
  GCCAGGCCTGCTGAGCCCTCCCAGTGCCTCCCCCTTTCCCACCCCTGCTGTCAGCCGGACCCTGCTGGGC
  AACTTTGAGGAATCACTGCTTCGAGGACGCTTTGCACCATCTGGCCACATTGAGGGCTTTACTGCAGAGA
  TTGGAGCCAGTGGGTCTTACTGTCCCCAACATGTCACGCTGCCCGTCACTGTCACCTTTTTTGATGTTTC
  TGAGCAAAATGCGCCGGCCCCCTTCCTGGGAGTCGTGGACCTGACTCCTCTGGGGAGGAAGGGTTACAGT
  GTGCCCAAGGTGGGCACCATCCAAGTGACCTTATTTAACCCCAACCAGACTGTGGTGAAGATGTTCCTTG
  TGACCTTTGACTTCTCGGACATGCCTGCTGCCCACATGACCTTCCTGCGCCATCGCCTCTTCTTGGTGCC
  TGTGGGTGAGGAGGGAAATGCTAGCCCCACCCACCGCCTCCTCTGCTACTTGCTGCACCTCAGGTTCCGG
  AGCTCTCGCTCAGGCCGCTTAAGCCTGCATGGAGATATCCGCCTGCTTTTTTCCCGCCGGAGCCTGGAGC
  TGGACACGGGGCTCCCCTATGAACTTCAGGCTGTGACTGAGGCCCCCCATAATCCACGTTATTCACCTTT
  GCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253354
• Insert Size: 1617 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253354.1, NP_001240283.1
• RefSeq Size: 3086 bp
• RefSeq ORF: 1617 bp
• Locus ID: 230088
• UniProt ID: Q8BR27
• Cytogenetics: 4 A5