Ugp2 (NM_001290634) Mouse Untagged Clone

CAT#: MC228031

Ugp2 (untagged) - Mouse UDP-glucose pyrophosphorylase 2 (Ugp2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ugp2
• Synonyms: UDPGP; UGPase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228031 representing NM_001290634
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCAAGATGGTGCTTCTCAGTTCCAAGAGGTCATTCTCCAAGAACTAGAATTATCTGTGAAGAAAG
  AATTAGAAAAAATACTTACCACAGCAGCCTCACATGAGTTTGAGCACACTAAAAAAGATCTTGATGGATT
  TCGGAAGCTATTTCATAGATTTTTGCAAGAAAAGGGACCGTCTGTGGACTGGGGTAAAATCCAGAGACCT
  CCAGAAGATTCGATTCAACCCTATGAAAAGATAAAAGCCAGAGGCTTGCCTGATAACATATCTTCTGTGT
  TGAACAAGTTGGTGGTAGTGAAACTCAATGGTGGTTTGGGAACCAGCATGGGCTGCAAAGGCCCTAAAAG
  TCTGATTGGTGTAAGAAATGAGAACACCTTTTTGGATCTAACTGTTCAGCAAATTGAACATTTGAACAAA
  ACCTATAATACAGATGTTCCTCTCGTTTTAATGAATTCTTTTAACACGGATGAAGATACCAAAAAAATAC
  TCCAGAAGTACAATCACTGTCGTGTGAAAATCTACACCTTCAATCAAAGCAGGTACCCGAGGATCAATAA
  GGAATCTCTACTTCCTATAGCAAAGGATGTGTCTTATTCAGGGGAAAATACTGAAGCTTGGTACCCTCCA
  GGGCATGGAGATATCTATGCTAGTTTCTACAATTCTGGCTTGCTCGATACCTTTATAGAAGAAGGCAAAG
  AGTATATTTTTGTGTCTAACATAGATAATCTGGGTGCCACAGTGGATCTTTATATTCTTAATCACCTGAT
  GAACCCACCCAATGGGAAGCGCTGTGAGTTTGTCATGGAAGTCACAAACAAAACACGAGCAGATGTTAAG
  GGTGGGACACTCACTCAGTATGAAGGCAAACTGAGACTGGTGGAAATCGCTCAAGTGCCCAAAGCACACG
  TTGATGAGTTCAAGTCTGTGTCAAAATTTAAAATATTTAACACAAACAACCTATGGATTTCTCTTGGAGC
  AGTTAAAAGACTTCAGGAACAGAATGCCATTGACATGGAAATCATTGTGAATCCAAAGACATTGGATGGA
  GGCCTGAATGTAATTCAGTTAGAAACTGCAGTTGGAGCTGCAATTAAAAGTTTCGAGAATTCTTTAGGTA
  TTAATGTTCCAAGGAGTCGTTTTCTGCCTGTGAAAACCACATCAGATCTCTTGCTTGTGATGTCAAACCT
  CTATAGCCTTAACGCAGGATCTCTGACCATGAGTGAAAAGCGTGAATTCCCTACAGTGCCCTTGGTTAAA
  TTAGGCAGTTCTTTTACCAAGGTTCAAGATTATCTAAGAAGGTTTGAAAGTATACCCGATATGCTGGAAC
  TGGACCACCTCACTGTTTCAGGAGATGTGACATTTGGAAAAAATGTTTCACTAAAGGGAACAGTTATTAT
  TATTGCAAATCATGGTGACAGAATTGACATCCCCCCAGGAGCCGTGTTAGAGAACAAGATTGTATCTGGA
  AACCTTCGGATCCTGGATCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290634
• Insert Size: 1494 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290634.1, NP_001277563.1
• RefSeq Size: 2657 bp
• RefSeq ORF: 1494 bp
• Locus ID: 216558
• UniProt ID: Q91ZJ5
• Cytogenetics: 11 13.89 cM
• Gene Summary: Plays a central role as a glucosyl donor in cellular metabolic pathways.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate 5'-terminal exon, differs in its 5' UTR, and uses a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.