Amigo1 (NM_146137) Mouse Untagged Clone

CAT#: MC227996

Amigo1 (untagged) - Mouse adhesion molecule with Ig like domain 1 (Amigo1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Amigo1
• Synonyms: ali2; Amigo
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227996 representing NM_146137
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAACCCCAGCGTGACCTGCGAGGCCTCTGGCTCCTGCTGCTCTCCGTGTTCCTGCTTCTCTTTGAGG
  TAGCCAGGGCCGGCCGATCTGTGGTTAGTTGTCCCGCCAACTGCCTGTGCGCCAGCAACATCCTCAGCTG
  CTCCAAGCAGCAGCTGCCCAATGTGCCCCAATCTTTGCCCAGCTACACAGCACTGCTGGACCTCAGCCAC
  AACAACTTGAGCAGGCTGCGGGCCGAGTGGACCCCCACCCGGCTGACCAACCTGCACTCCCTGCTGCTGA
  GCCACAACCACCTGAACTTCATCTCCTCCGAGGCCTTCGTCCCCGTACCCAACCTTAGGTACTTGGACCT
  CTCCTCCAACCATCTTCACACGCTGGATGAGTTCCTGTTCAGCGACCTGCAGGCGCTGGAAGTGCTGTTG
  CTCTACAATAACCACATTGTGGTGGTGGACCGGAATGCCTTTGAGGACATGGCCCAGCTGCAGAAACTCT
  ACTTAAGCCAGAATCAGATCTCTCGCTTTCCTGTGGAACTGATCAAGGATGGGAACAAATTACCCAAACT
  GATGCTCTTGGATCTGTCCTCCAACAAGCTGAAGAAGTTGCCCCTGACTGACCTGCAGAAATTGCCAGCC
  TGGGTCAAGAATGGGCTATACCTGCATAACAACCCCTTGGAGTGCGACTGCAAGCTCTACCAGCTCTTTT
  CGCACTGGCAGTACCGGCAGCTGAGCTCTGTGATGGACTTCCAGGAGGACCTGTACTGCATGCACTCCAA
  GAAGCTGCACAACATCTTCAGCCTGGATTTCTTCAATTGCAGCGAGTACAAGGAAAGTGCCTGGGAGGCT
  CACCTGGGAGACACCTTGACCATCAGGTGTGACACCAAACAGCAAGGCATGACCAAAGTGTGGGTGTCCC
  CAAGCAATGAACAGGTGCTAAGTCAGGGGTCCAATGGCTCGGTGAGCGTGAGGAATGGCGACCTTTTTTT
  TAAAAAGGTGCAGGTCGAGGATGGGGGTGTGTATACCTGTTACGCCATGGGGGAGACTTTCAACGAGACA
  CTGTCTGTGGAGTTGAAAGTGTATAACTTCACCTTGCACGGACACCATGACACCCTCAACACAGCCTACA
  CTACCCTGGTGGGCTGTATCCTCAGTGTGGTTCTGGTCCTCATATACTTGTACCTCACCCCTTGCCGCTG
  CTGGTGTCGGGGTGTGGAGAAACCTTCCAGCCACCAAGGAGATAGCCTCAGCTCTTCTATGCTCAGTACC
  ACACCCAACCACGACCCTATGGCTGGTGGGGACAAAGATGATGGTTTTGACCGGCGGGTGGCCTTCCTGG
  AACCTGCTGGACCCGGGCAGGGTCAAAATGGCAAACTCAAGCCAGGCAACACTCTGCCGGTGCCCGAAGC
  TACAGGCAAGGGCCAACGGAGGATGTCCGATCCAGAGTCGGTCAGCTCGGTCTTTTCTGATACACCCATT
  GTGGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_146137
• Insert Size: 1479 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146137.3, NP_666249.2
• RefSeq Size: 5586 bp
• RefSeq ORF: 1479 bp
• Locus ID: 229715
• UniProt ID: Q80ZD8
• Cytogenetics: 3 F2.3
• Gene Summary: Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain (By similarity). Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (PubMed:22056818).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.