Agfg2 (NM_001303266) Mouse Untagged Clone

CAT#: MC227979

Agfg2 (untagged) - Mouse ArfGAP with FG repeats 2 (Agfg2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Agfg2
• Synonyms: A630095P14Rik; Hr; Hrbl; RAB-R; RABR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227979 representing NM_001303266
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGATGGCGGCTAAGAAGGGCCCGGGCCCGGGTGGCGGGGTCGGAGGGAGCAAAGCGGAGGCTGAAG
  CCGCTTCGGAGGTGTGGTGCCGCCGAGTGCGGGAGCTGGGCGGCTGCAGCCAGGCCGGGAACCGCCACTG
  TTTCGAGTGCGCCCAGCGCGGGGTCACGTATGTGGACATCACCGTGGGCAGCTTCGTCTGCACCACCTGC
  TCCGGCCTCCTGAGAGGCCTGAACCCCCCTCATCGAGTCAAGTCAATCTCCATGACAACTTTCACTGAGC
  CTGAAGTCCTGTTCCTCCAATCTCGTGGAAATGAGGTCTGTCGGAAAATCTGGCTCGGTCTTTTTGATGC
  TCGGACATCGTTGATACCAGATTCCAGGGATCCTCAGAAGGTGAAGGAGTTTCTCCAAGAAAAATATGAG
  AAAAAAAGATGGTACGTCCCCCCAGAGCAAGTCAAGGGGCCCTCTTACAGCAAAGGCAGTGTCTCTGCTA
  CCCCTGTCCAGGGCTCTGTCCCAGAAGGGAAACCCATTCGGACACTTCTGGGAGACCCTGTGCCATCTCT
  CTCTGATCCTGCTTCCACTTCAAGCCAGCCTGGGAGCCAGTCGCAGGCACGCAGCAGCTCGCAGGCCAGG
  AGCTCCCAGCCTCCTTCCCATTCATCCACCAAGAAAGCCAGCACTGACCTGCTGGCGGATATCGGGGGAG
  ACCCCTTTGCTGCTCCCCAGGTGGTGCCAGCCTTTGCCTCATTCCCAGGCTTTGGAGTAGGCCAGACTCC
  TGCCCATGGAGGCTTTGCCAACTTCGATGCCTTCAGCAGCAGCCCTAGCTCTTCCACCTTCGGAAGCCTC
  CCTCCATCCGTCCAAGCGCCATTCCAGGCCCAGCCGACCCCTGCAGCCAATCGGATGCTAACTGGAAGTT
  ACAGCTTTGGAAGTGGCCAGATGTCTGCGTTTGGTGTGGCACCCCTTGCAGCTGCCAGTCAACCCAACAA
  CCTTGCAGATGTGGGCGGCCTCCTGGGTCCCAGGATGGCTGCTGGAGGTCTCCCTGGCAGTGTTTTTGGG
  ATGCCGAGCCAGGTTCCTGCCCTGCAGTCGGCCGTGCCAGGTGTTAGCGGCAGTGGAGGGCTCCCCTTTG
  GAGCCTACACCAACCCCTTCGCCACCCCTGCCCAAGCCCAGCTGCCTTCTACCAACCCATTCCAACCCAA
  TGGTCTAGCCTCAGGGCCTGGCTTTGGGATGAGCAGTGTTCGGCCTGGCCTTCTCCAGCCAGTGCCACCC
  TCCGGGGCCTTTGCCAGTCCCTTCTCTGCACCCGTGTTCCCCACACAGGCTGGACTGGCCGACCAGCAGA
  ATGGATCTTCCTTTGGCGACTTGGGGACCTCTAAGCTGGGGCAGAGGCCACTGAGCCAGCCGGCTGGGAT
  CTCTACCAATCCTTTCATGACTGGATCCTCAGCGTTTGCCTCCAAACCTCCAACCACAAACCCATTCTTG
  TAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001303266
• Insert Size: 1473 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001303266.1, NP_001290195.1
• RefSeq Size: 2953 bp
• RefSeq ORF: 1473 bp
• Locus ID: 231801
• Cytogenetics: 5 G2
• Gene Summary: This gene encodes a paralog of the HIV-1 Rev binding proteins that serve as cellular co-factors for HIV-1 Rev protein in shuttling viral pre-mRNAs from the nucleus to the cytoplasm. The encoded protein contains an ADP-ribosylation factor GTPase activating protein (Arf-GAP) zinc finger domain, several phenylalanine-glycine (FG) motifs and asparagine-proline-phenylalanine (NPF) motifs. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014]
  Transcript Variant: This variant (3) contains an additional in-frame exon in the 3' coding region, compared to variant 2. The resulting protein (isoform 3) is longer than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.