Micu1 (NM_001291443) Mouse Untagged Clone

CAT#: MC227923

Micu1 (untagged) - Mouse mitochondrial calcium uptake 1 (Micu1), transcript variant 2


  "NM_001291443" in other vectors (1)

Reconstitution Protocol

USD 503.00

2 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Other products for "Micu1"

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Micu1
Synonyms C730016L05Rik; Calc; Cbara1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227923 representing NM_001291443
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTCGTCTTAACACCCTTTCTGCGTTGGCAGAACTAGCTGTGGGCTCTCGCTGGTACCATGGGGCAT
CACAGCCCACACAGACCAAACGAAGATTGATGCTGGTGGCGTTCCTGGGAGCATCTGCAGTGACTGCAAG
TACCGGTCTCCTGTGGAAGAAGGCTCACGCAGAATCTCCACCATGCGTTAACAGCAAGAAGCCTGACACA
GAAGATAAAGAGAGAAACAAAGACAGCGGGGAAGTGTCCAGCCGTGAAGGAAGGGCTGCAGATGCTGCGG
CAGAGCCTTATCCTGAGGACAAGAAGAAGAAGCGCTCTGGATTCAGAGACAGAAAAGTGATGGAGTATGA
GAATCGAATCCGAGCCTACTCCACACCAGACAAAATTTTCCGATATTTTGCCACCTTGAAAGTAATCAAC
GAACCTGGTGAAACCGAAGTGTTCATGACTCCGCAGGACTTTGTGCGCTCCATAACGCCCAATGAGAAGC
AGCCAGAACACTTGGGCCTGGATCAGTATATAATAAAGCGCTTTGATGGAAAGGAGTTCTGGCAGAAAAT
TGCCCAGGAACGAGAAAAATTTGCTGATGAAGGCAGCATCTTCTACAGCCTTGGAGAGTGTGGGCTCATC
TCCTTCTCTGACTACATCTTCCTCACAACAGTCCTCTCCACTCCTCAGAGAAATTTTGAAATTGCTTTCA
AGATGTTTGACTTGAATGGAGACGGAGAGGTAGACATGGAGGAGTTTGAGCAGGTTCAGAGCATCATTCG
CTCCCAGACCAGCATGGGCATGCGTCACAGAGACCGTCCAACCACTGGGAACACCCTCAAGTCTGGCTTA
TGTTCGGCCCTCACGACCTACTTTTTTGGAGCTGATCTCAAAGGGAAACTGACCATCAAAAACTTCCTGG
AATTTCAGCGCAAACTTCAGCATGACGTTCTAAAGCTGGAGTTTGAACGCCATGACCCGGTAGATGGGAG
AATCTCTGAGAGGCAGTTCGGGGGCATGCTGCTGGCCTACAGTGGAGTGCAGTCCAAGAAGCTGACCGCC
ATGCAGAGACAGCTGAAGAAGCACTTCAAAGATGGGAAGGGCCTGACTTTCCAGGAGGTAGAGAACTTCT
TCACTTTCCTGAAGAACATTAATGACGTGGACACTGCACTAAGCTTTTACCACATGGCTGGAGCATCTCT
TGATAAAGTGACCATGCAGCAAGTGGCCAGGACAGTGGCGAAGGTCGAACTCTCGGACCATGTGTGTGAC
GTGGTGTTCGCGCTCTTTGACTGTGATGGCAATGGGGAGCTGAGCAACAAGGAGTTCGTTTCCATCATGA
AGCAGCGGCTGATGAGAGGCCTGGAGAAGCCCAAGGACATGGGCTTCACCCGCCTCATGCAGGCCATGTG
GAAATGTGCCCAAGAAACTGCCTGGGACTTTGCTCTGCCCAAATAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001291443
Insert Size 1446 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001291443.1, NP_001278372.1
RefSeq Size 2370 bp
RefSeq ORF 1446 bp
Locus ID 216001
UniProt ID Q8VCX5
Cytogenetics 10 B4
Gene Summary Key regulator of mitochondrial calcium uniporter (MCU) that senses calcium level via its EF-hand domains (PubMed:24560927). MICU1 and MICU2 form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium (PubMed:24560927). MICU1 acts both as an activator or inhibitor of mitochondrial calcium uptake (By similarity). Acts as a gatekeeper of MCU at low concentration of calcium, preventing channel opening (By similarity). Enhances MCU opening at high calcium concentration, allowing a rapid response of mitochondria to calcium signals generated in the cytoplasm (PubMed:24560927). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (By similarity). Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) lacks an alternate exon in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.