Cacnb4 (NM_001285427) Mouse Untagged Clone

CAT#: MC227871

Cacnb4 (untagged) - Mouse calcium channel, voltage-dependent, beta 4 subunit (Cacnb4), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cacnb4
• Synonyms: 3110038O15Rik; Cchb4; lh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227871 representing NM_001285427
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGGTCAGCAGATTCCTATACAAGCAGGCCATCGGACTCCGATGTCTCCTTGGAAGAGGACCGGG
  AAGCGATCCGGCAAGAGCGAGAACAGCAAGCAGCTATCCAGCTTGAGAGAGCGAAGTCCAAACCTGTAGC
  TTTTGCGGTGAAAACGAACGTGAGCTACTGTGGTGCCCTGGACGAAGATGTGCCCGTTCCCAGCACAGCC
  ATCTCCTTTGACGCCAAGGACTTTCTTCACATTAAAGAGAAATATAACAATGATTGGTGGATAGGAAGAC
  TGGTAAAAGAGGGCTGTGAGATTGGCTTCATCCCAAGTCCACTGCGCTTGGAGAATATTCGGATTCAACA
  GGAACAGAAAAGAGGCCGTTTTCATGGCGGGAAATCGAGTGGAAACTCTTCCTCCAGTCTGGGAGAAATG
  GTATCAGGAACATTCCGAGCAACTCCCACAACAACAGCAAAACAGAAGCAGAAAGTGACGGAGCACATTC
  CTCCGTATGACGTCGTGCCGTCAATGCGTCCTGTGGTGTTAGTGGGGCCATCACTGAAAGGTTATGAGGT
  AACAGACATGATGCAGAAAGCCCTCTTTGATTTCCTGAAGCACAGGTTTGATGGGAGGATATCAATAACA
  AGAGTGACAGCTGACATTTCTCTTGCTAAGAGATCTGTCCTCAACAATCCTAGCAAGAGAGCAATAATTG
  AACGTTCCAACACCAGATCCAGCTTAGCGGAAGTACAAAGTGAAATTGAAAGAATTTTTGAGTTGGCAAG
  ATCTTTGCAATTGGTTGTTCTTGATGCAGACACCATCAACCACCCAGCACAGCTGATAAAGACATCCTTA
  GCACCCATCATCGTCCACGTGAAGGTCTCGTCCCCAAAGGTTTTACAGCGGCTGATTAAGTCCAGAGGAA
  AGTCCCAAAGCAAACACTTGAATGTTCAACTGGTGGCGGCCGATAAACTGGCCCAGTGCCCGCCTGAAAT
  GTTTGATGTTATATTAGATGAGAATCAACTTGAGGATGCCTGTGAACATCTGGGAGAGTACCTGGAGGCA
  TACTGGCGTGCCACCCACACGAGCAGTAGCACCCCTATGACCCCATTACTGGGGCGGAACGTGGGCTCCA
  CAGCCCTCTCACCATATCCCACAGCAATCTCTGGATTACAGAGTCAGCGAATGAGACACAGCAACCATTC
  TACAGAGAATTCTCCAATTGAAAGACGAAGCCTAATGACCTCGGATGAAAATTACCACAATGAGAGGGCC
  CGCAAGAGTAGGAACCGCTTGTCTTCCAGCTCCCAGCACAGCCGAGACCACTACCCTCTGGTGGAAGAAG
  ATTACCCGGACTCGTACCAGGACACTTATAAGCCCCATAGGAACCGAGGATCGCCCGGGGGGTGCAGCCA
  TGACTCCCGACATAGGCTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285427
• Insert Size: 1422 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285427.1, NP_001272356.1
• RefSeq Size: 8088 bp
• RefSeq ORF: 1422 bp
• Locus ID: 12298
• UniProt ID: Q8R0S4
• Cytogenetics: 2 29.98 cM
• Gene Summary: The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR and lacks a portion of the 5' coding region compared to variant 1. This results in the use of a downstream start codon. The resulting protein (isoform d) is shorter and has a distinct N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.