Cacnb1 (NM_001282978) Mouse Untagged Clone

CAT#: MC227864

Cacnb1 (untagged) - Mouse calcium channel, voltage-dependent, beta 1 subunit (Cacnb1), transcript variant 6

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cacnb1
• Synonyms: CAB1; Cchb1; Cchlb1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227864 representing NM_001282978
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCAGAAGAGCGGCATGTCCCGGGGCCCTTACCCACCTTCCCAAGAGATCCCTATGGAGGTCTTCG
  ACCCCAGCCCACAGGGCAAGTACAGCAAGAGGAAAGGGCGGTTCAAAAGGTCAGACGGGAGTACGTCCTC
  GGATACAACATCCAACAGCTTCGTCCGCCAGGGCTCAGCAGAGTCCTACACGAGCCGACCATCAGACTCT
  GATGTGTCTCTGGAGGAGGACCGGGAAGCCTTAAGGAAGGAGGCAGAGCGCCAGGCCTTAGCCCAGCTCG
  AGAAAGCCAAGACCAAACCAGTGGCTTTTGCTGTTCGGACAAATGTTGGCTACAATCCGTCTCCAGGGGA
  TGAGGTGCCTGTACAGGGAGTGGCCATCACCTTTGAGCCCAAGGACTTCCTACACATCAAGGAGAAGTAC
  AATAATGACTGGTGGATTGGGCGGCTGGTGAAGGAAGGCTGCGAGGTTGGCTTCATCCCCAGCCCGGTCA
  AACTGGACAGCCTTCGTCTGCTGCAGGAACAGACCCTGCGCCAGAACCGCCTCAGCTCCAGCAAGTCAGG
  TGACAACTCCAGTTCCAGTCTGGGAGATGTGGTGACTGGCACCCGCCGCCCCACACCCCCTGCCAGTGAG
  CACGTGCCCCCCTATGACGTGGTGCCTTCCATGAGGCCCATCATCCTGGTGGGACCGTCGCTCAAGGGCT
  ATGAGGTGACAGACATGATGCAGAAAGCGTTGTTTGACTTCCTCAAGCATCGGTTTGATGGCAGGATTTC
  CATCACCCGGGTAACAGCTGACATTTCCCTGGCCAAACGCTCCGTCCTCAACAACCCCAGCAAACACATC
  ATCATTGAGCGCTCCAACACGCGTTCCAGCCTGGCTGAGGTACAGAGTGAAATTGAGAGGATCTTCGAGC
  TGGCCCGGACCTTGCAGCTGGTCGCCTTGGACGCTGACACCATCAACCACCCAGCCCAGCTCTCTAAAAC
  GTCGCTGGCCCCCATCATTGTTTACATCAAGATCACATCTCCCAAGGTACTGCAGAGGCTCATCAAATCC
  CGAGGGAAGTCTCAATCCAAACACCTCAATGTCCAAATAGCAGCCTCGGAGAAGCTGGCACAGTGTCCCC
  CCGAAATGTTTGACATAATCCTGGACGAGAACCAATTGGAAGATGCCTGCGAGCACCTGGCTGAGTACTT
  GGAAGCCTACTGGAAGGCCACACATCCGCCTAGCAGCACGCCACCCAATCCGCTGCTGAACCGCACCATG
  GCTACCGCAGCTCTGGCTGCCAGCCCTGCCCCCGTCTCCAACCTCCAGGTACAGGTGCTCACCTCGCTCA
  GGAGAAATCTCAGCTTCTGGGGCGGGCTGGAGGCCTCACCGCGGGGAGGCGACGCGGTGGCCCAGCCTCA
  GGAGCACGCCATGTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001282978
• Insert Size: 1416 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282978.1, NP_001269907.1
• RefSeq Size: 1714 bp
• RefSeq ORF: 1416 bp
• Locus ID: 12295
• Cytogenetics: 11 61.5 cM
• Gene Summary: Regulatory subunit of L-type calcium channels. Regulates the activity of L-type calcium channels that contain CACNA1A as pore-forming subunit (By similarity). Regulates the activity of L-type calcium channels that contain CACNA1C as pore-forming subunit and increases the presence of the channel complex at the cell membrane. Required for functional expression L-type calcium channels that contain CACNA1D as pore-forming subunit. Regulates the activity of L-type calcium channels that contain CACNA1B as pore-forming subunit (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (6) lacks an alternate coding exon, uses an alternate splice junction at the 5' end of an exon, and differs in the 3' UTR and coding sequence compared to variant 5. The resulting isoform (F) has a shorter and distinct C-terminus and lacks an alternate internal segment compared to isoform E. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.