Plin5 (NM_025874) Mouse Untagged Clone

CAT#: MC227824

Plin5 (untagged) - Mouse perilipin 5 (Plin5), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Plin5
• Synonyms: 2310076L09Rik; AI415325; AW109675; Lsdp5; MLDP; PAT-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227824 representing NM_025874
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCAGAGAGGTGAAGACACCACCCTAGCGCCACACAGCAGAATGTCCGGTGATCAGACAGCTCAGG
  ACCCTGGATCCAGCCTGGGAGAACTGGACCAGCAGAATGTGGTGAATCGAGTGGTGGCTTTGCCCCTGGT
  CAAGGCCACGTGCACTGCCGTGTCCAGTGCTTACAACTCGGCCAAGGACAGGCACCCGCTGCTGGGCTCC
  GCCTGCCGCCTTGCTGAGCACTGTGTGTGTAGTGTGACTACCTGTGCCCTGGACCACGCACAGCCACTGC
  TGGAGCACCTGCAGCCCCAGTTGGCCACAGTGAATGATCTTGCCTGCAGGGGACTAGACAAATTGGAAGA
  GAAGCTGCCCTTCCTGCAGCAGCCATCAGACATGGTGGTGACATCAGCCAAGGATACAGTGGCCAAAAGT
  GTCACAGGCATGGTGGACCTGGCCCAAAGGGGCCGGCGTTGGAGTGGGGAGCTGAGGCGCTCCATGAGTC
  AAGCCATGGACATGGTGCTGGGCAAGTCGGAGAAGCTGGTGGACCGCTTCCTGCCCATGACTGAGGCTGA
  GCTAGCAGTCCTGGCAGCTGAGGCCGAGGGCCCAGAAGTGGGCACAGTGGAGGAGCAGAGGCAGCAACAG
  GGCTACTTTGTGCGTCTGGGGTCCCTATCGGCACGCCTCCGCCATCTCGCCTATGAACACTCTTTGGGGA
  AACTGAGGCAGAGCAAACACCGTACCCAGGAGATGCTGGCCCAGCTGCAGGAAACGCTGGAGCTGATCCA
  GCATATGCAGAGAGGGGCAAGCCCTAGCCCTACTTTCCATCCCCCAAAGACTCAGGAGCTGTGGGGGAGC
  TGGAGCCCGTGTCTAGAGAATGGCCGCAGCCACAGTGAGGTGGAGCTGGAGACACTGGCTCTGTCTCGAA
  GTTTGACCCTGGAGCTGCAGAATGCAGTGGATGCCCTGGCAGGCTGTGTTCGGGGCCTGCCACCTAGTGC
  CCAGGCCAAGGTGGCTGAGGTGCAGCGCAGCGTGGATGCTCTACAGGCCACCTTTGCTGATGCACACTGC
  CTTGGTGATGTGGCACCCACTGCTCTGGCTGAGGGCCGGGGCAGTGTGGCCCGGGCACATGCCTGTGTGG
  ATGAGTTCCTGGATTTGGTCCTGCGGGCCATGCCACTGCCCTGGCTTGTGGGGCCCTTTGCACCCATCCT
  GGTGGAACAGTCGGAGCCCCTGATCAACCTGGCCACCTGTGTGGACGAGGTGGTGGGTGACCCTGATCCT
  CGCTGGGCACACATGGACTGGCCAGCCCAGAAGAGGGCCTGGGAGGCTGAGTCTGCAGATCCTGGGGGGC
  AAGAGGCTGAGCCCCCAAGGGGGCAAGGCAAGCACACAATGATGCCAGAGCTGGACTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025874
• Insert Size: 1392 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025874.3, NP_080150.2
• RefSeq Size: 1940 bp
• RefSeq ORF: 1392 bp
• Locus ID: 66968
• UniProt ID: Q8BVZ1
• Cytogenetics: 17 D
• Gene Summary: Lipid droplet-associated protein that maintains the balance between lipogenesis and lipolysis and also regulates fatty acid oxidation in oxidative tissues. Recruits mitochondria to the surface of lipid droplets and is involved in lipid droplet homeostasis by regulating both the storage of fatty acids in the form of triglycerides and the release of fatty acids for mitochondrial fatty acid oxidation. In lipid droplet triacylglycerol hydrolysis, plays a role as a scaffolding protein for three major key lipolytic players: ABHD5, PNPLA2 and LIPE. Reduces the triacylglycerol hydrolase activity of PNPLA2 by recruiting and sequestering PNPLA2 to lipid droplets. Phosphorylation by PKA enables lipolysis probably by promoting release of ABHD5 from the perilipin scaffold and by facilitating interaction of ABHD5 with PNPLA2. Also increases lipolysis through interaction with LIPE and upon PKA-mediated phosphorylation of LIPE.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the predominant transcript. Variants 1 and 2 encode the same protein.