Sox17 (NM_001289464) Mouse Untagged Clone

CAT#: MC227603

Sox17 (untagged) - Mouse SRY (sex determining region Y)-box 17 (Sox17), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sox17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227603 representing NM_001289464
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGCCCGGATGCGGGATACGCCAGTGACGACCAGAGCCAGCCCCGGAGCGCGCAGCCCGCGGTGA
  TGGCAGGGTTGGGCCCCTGTCCCTGGGCCGAGTCCCTGAGCCCCCTCGGGGATGTAAAGGTGAAAGGCGA
  GGTGGTGGCGAGTAGCGGGGCGCCAGCCGGGACGTCGGGCCGAGCCAAAGCGGAGTCTCGCATCCGGCGG
  CCGATGAACGCCTTTATGGTGTGGGCCAAAGACGAACGCAAGCGGTTGGCACAGCAGAACCCAGATCTGC
  ACAACGCAGAGCTAAGCAAGATGCTAGGCAAGTCTTGGAAGGCGTTGACCTTGGCAGAGAAGCGGCCCTT
  CGTGGAAGAGGCCGAGCGGCTGCGCGTGCAGCATATGCAGGACCACCCCAACTACAAGTACCGGCCGCGG
  CGGCGCAAGCAGGTGAAGCGCATGAAGCGGGTGGAGGGAGGCTTCCTGCACGCTCTCGTCGAGCCCCAGG
  CCGGCGCGCTTGGTCCCGAGGGCGGCCGCGTGGCCATGGATGGCCTGGGTCTGCCTTTCCCGGAGCCGGG
  CTATCCGGCCGGTCCTCCGCTGATGTCTCCGCACATGGGCCCCCACTATCGGGACTGCCAGGGACTGGGC
  GCTCCCGCGCTCGACGGCTACCCTCTGCCCACTCCGGACACATCCCCGCTGGATGGCGTGGAGCAGGACC
  CGGCTTTCTTTGCAGCCCCGCTGCCAGGGGACTGCCCGGCGGCCGGCACCTACACTTACGCTCCAGTCTC
  GGACTATGCAGTGTCCGTAGAGCCGCCCGCTGGCCCCATGCGAGTGGGGCCGGACCCCTCGGGCCCTGCG
  ATGCCGGGGATCCTGGCGCCCCCCAGCGCTCTGCACCTGTACTACGGCGCGATGGGCTCGCCCGCCGCAA
  GTGCGGGGCGCGGTTTCCACGCGCAACCCCAGCAGCCGCTGCAACCGCAGGCACCGCCGCCGCCACCGCA
  GCAGCAGCACCCAGCGCACGGCCCCGGGCAACCTTCGCCCCCTCCCGAGGCTCTGCCCTGCCGGGATGGC
  ACGGAATCCAACCAGCCCACTGAGCTCCTAGGGGAGGTGGACCGCACGGAATTCGAACAGTATCTGCCCT
  TTGTGTATAAGCCCGAGATGGGTCTTCCCTACCAGGGACACGACTGCGGAGTGAACCTCTCAGACAGCCA
  CGGAGCCATTTCCTCCGTGGTGTCCGACGCTAGCTCAGCGGTCTACTATTGCAACTACCCCGACATTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289464
• Insert Size: 1260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289464.1, NP_001276393.1
• RefSeq Size: 3288 bp
• RefSeq ORF: 1260 bp
• Locus ID: 20671
• UniProt ID: Q61473
• Cytogenetics: 1 1.65 cM
• Gene Summary: This gene encodes a member of the Sox (Sry-related high mobility group box) family of transcription factors involved in the regulation of embryonic development. The encoded protein plays a role in the determination of cell fate and in maintaining cell identity. This gene regulates tumor angiogenesis and tumor progression. Mutations in the human gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein (isoform a, also known as Sox17; PMID 8636240).