Rxrb (NM_001205215) Mouse Untagged Clone

CAT#: MC227569

Rxrb (untagged) - Mouse retinoid X receptor beta (Rxrb), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rxrb
• Synonyms: AL023085; H-2RIIBP; Nr2b2; RCoR-1; Rub
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227569 representing NM_001205215
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACCCCCGCCACTGGGCTCCCCCTTCCCAGTCATCAGTTCTTCCATGGGGTCCCCTGGTCTGCCCC
  CTCCGGCTCCCCCAGGATTCTCCGGGCCTGTCAGCAGCCCTCAGATCAACTCCACAGTGTCGCTCCCTGG
  GGGTGGGTCTGGCCCCCCTGAAGATGTGAAGCCACCGGTCTTAGGGGTCCGGGGCCTGCACTGTCCACCC
  CCTCCAGGTGGTCCTGGGGCTGGCAAACGGCTCTGTGCAATCTGCGGGGACCGAAGCTCAGGCAAGCACT
  ATGGGGTTTACAGCTGCGAGGGCTGCAAGGGTTTCTTCAAGCGCACCATTCGGAAGGACCTGACCTACTC
  GTGTCGTGATAACAAAGACTGTACAGTGGACAAGCGCCAGCGGAATCGCTGTCAGTACTGTCGCTATCAG
  AAGTGCCTGGCCACTGGCATGAAAAGGGAGGCGGTTCAGGAGGAGCGTCAACGGGGGAAGGACAAAGACG
  GGGATGGAGATGGGGCTGGGGGAGCCCCTGAGGAGATGCCTGTGGACAGGATCCTGGAGGCAGAGCTTGC
  TGTGGAGCAGAAGAGTGACCAAGGCGTTGAGGGTCCTGGGGCCACCGGGGGTGGTGGCAGCAGCCCAAAT
  GACCCAGTGACTAACATCTGCCAGGCAGCTGACAAACAGCTGTTCACACTCGTTGAGTGGGCAAAGAGGA
  TCCCGCACTTCTCCTCCCTACCTCTGGACGATCAGGTCATACTGCTGCGGGCAGGCTGGAACGAGCTCCT
  CATTGCGTCCTTCTCCCATCGGTCCATTGATGTCCGAGATGGCATCCTCCTGGCCACGGGTCTTCATGTG
  CACAGAAACTCAGCCCATTCCGCAGGCGTGGGAGCCATCTTTGATCGGTCCCTCTCCAGGGTGCTGACAG
  AGCTAGTGTCCAAAATGCGTGACATGAGGATGGACAAGACAGAGCTTGGCTGCCTGCGGGCAATCATACT
  GTTTAATCCAGACGCCAAGGGCCTCTCCAACCCTGGAGAGGTGGAGATCCTTCGGGAGAAGGTGTACGCC
  TCACTGGAGACCTATTGCAAGCAGAAGTACCCTGAGCAGCAGGGCCGGTTTGCCAAGCTGCTGTTACGTC
  TTCCTGCCCTCCGCTCCATCGGCCTCAAGTGTCTGGAGCACCTGTTCTTCTTCAAGCTCATTGGCGACAC
  CCCCATTGACACCTTCCTCATGGAGATGCTTGAGGCTCCCCACCAGCTAGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001205215
• Insert Size: 1245 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205215.1, NP_001192144.1
• RefSeq Size: 2356 bp
• RefSeq ORF: 1245 bp
• Locus ID: 20182
• Cytogenetics: 17 17.98 cM
• Gene Summary: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.