Cipc (NM_001289430) Mouse Untagged Clone

CAT#: MC227554

Cipc (untagged) - Mouse CLOCK interacting protein, circadian (Cipc), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cipc
• Synonyms: 2310044G17Rik; AI842544; AI853744; Kiaa1737; mKIAA1737
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227554 representing NM_001289430
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAAGAGTACCTGTGCTCCGTCTTCCGGCCATCTTAGACATGGAGAGGAAAATCCCATCCAGAGAGA
  GCCCCAGAAGACTGTCCGCCAAGCCAGGCCGAGGCACGGAGATGAAGAAGCTAGCGCGACCACTTGGCGT
  GGTGGCAGCTGACTCAGACAAGGACTCTGGCTTCTCAGATGGGAGCTCAGAATGTTTGAGCTCCGCAGAG
  CAGATGGAGTCAGAGGACATGCTGAGCGCCTTGGGCTGCAAGAGAGAAGACAAGCGGAGGCAGCCCTCCA
  AAGCTGCGGACACTGCCCTCCCCACGCTGCCCCCTATGGTCGTCATGAAGAGCGTGCTGGTCAAGCAGGG
  CAGCAGCTCCTCCCAGCTTCAGTCCTGGACTGTCCAGCCCTCCTTTGAAGTGATCTCAGCGCAGCCACAG
  CTCTTTGTCCTTCATCCACCTGTGCCATCTCCTGTCAGCTCATGCCAGACTGGTGAGAAAAAGTCAGAAT
  CCAGAAACTACTTGCCCATTCTAAATTCTTATACCAAAATAGCCCCCCACCCAGGCAAGAGGGGCCTCAA
  TTCAGAAGACAGAGGAACAAGTGGGGTATCGAAGAAACTCTGCACGGAGAGACCGGGGCCAAGCCTGTCT
  TCCAGTGAGCCAGCCAAGACTGGCCGAGTATTATCCAGTCCTTCCACTCCAGCCCCACCCAGCTCCAAAC
  TCACTGAGGACTCCACTCTGCAAGGAGTTCCCTCCCTTGGGGCTGGCGGAAGTCCACAGACTCTTCAGCC
  TGTGTCCAGCAGCCATGTGGCTAAAGCTCCCAGTCTGACTTTGGCTTCGCCCGCCAGCCCTGTGTGTGCG
  TCAGACAGCACTCTGCATGGCCTAGAGAGCAGCTCCCCTCTCTCCCCACTGTCAGCCAGTTACACTTCAC
  CTCTGTGGGCTGCGGAGCACCTCTGCCGCAGCCCAGACATCTTCTCCGAGCAGAGGCAGAACAAGCATAG
  GCGCTTTCAGAATACCTTAGTGGTCCTCCACAAGTCTGGTTTGCTGGAGATCACTCTGAAAACCAAGGAG
  TTGATTCGTCAGAACCAAGCAACTCAGGCAGAGCTGGACCAGCTGAAGGAGCAAACCCAGATGTTTATAG
  AGGCCACCAAGAGCAGGGCTCCTCAGGCATGGGCCAAGTTGCAGGCATCACTAACATCTGGGTCCAGTCA
  TTCAGGCAGTGACCTAGACACGTTGTCTGATCACCCAGACGTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289430
• Insert Size: 1236 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289430.1, NP_001276359.1
• RefSeq Size: 4169 bp
• RefSeq ORF: 1236 bp
• Locus ID: 217732
• UniProt ID: Q8R0W1
• Cytogenetics: 12 D2
• Gene Summary: Transcriptional repressor which may act as a negative-feedback regulator of CLOCK-ARNTL/BMAL1 transcriptional activity in the circadian-clock mechanism. May stimulate ARNTL/BMAL1-dependent phosphorylation of CLOCK (PubMed:17310242, PubMed:19414601). However, the physiogical relevance of these observations is unsure, since experiments in knockout mice showed that CIPC is not critially required for basic circadian clock (PubMed:25862660).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Both variants 1 and 3 encode isoform a.