Azi2 (NM_001286508) Mouse Untagged Clone

CAT#: MC227522

Azi2 (untagged) - Mouse 5-azacytidine induced gene 2 (Azi2), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Azi2
• Synonyms: AA410145; AZ2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227522 representing NM_001286508
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACGCTAGTAGAAGATGACATCTGCATTCTGAATCATGAAAAGGCCCACAGGAGAGAGGCAGTGA
  CTCCACTCTCAGCGTACCCTGGAGATGAGTCTGTTGCTTCCCATTTCGCACTGGTCACTGCTTATGAAGA
  CATCAAAAAGAGGCTGAAGGATTCAGAGAAAGAAAACTCCTTCTTAAAGAAAAGAATCAGGGCTTTGGAA
  GAAAGGCTTGTTGGAGCTCGAGCAGATGAAGAAACAAGTTCTGTGGGCCGAGAACAAGTGAATAAGGCCT
  ATCATGCATATCGTGAGGTTTGCATTGATAGAGACAATCTGAAGAACCAATTGGAAAAGATAAATAAAGA
  CAACTCTGAATCTTTGAAAATGTTGAATGAGCAGCTACAGTCTAAAGAAGTAGAACTTCTGCAGCTAAGA
  ACAGAGGTGGAGACTCAGCAGGTGATGAGGAATTTAAACCCACCCTCATCCAGCTGGGAGGTGGAGAAGC
  TAAGCTGTGACTTGAAGATCCATGGTTTGGAGCAAGAGCTGGGACTACTGAGGAAGGAGTGCAGTGACCT
  CAGGACAGAGCTGCAGAAGGCCAGACAGACGGGTCCACCTCAGGAAGACATTCTGCAGGGCAGAGATGTC
  ATCAGGCCGAGCTTGTCGAGAGAAGAGCATGTTCCACACCAGGGTCTTCACCACAGTGATAATATGCAAC
  ATGCATACTGGGAACTGAAGAGGGAGATGTCTAACTTACACCTGGTGACTCAAGTGCAAGCAGAACTACT
  AAGAAAACTGAAAACCTCAGCTGCAGTCAAGAAAGCCTGTACCCCAGTAGGATGTGTTGAAGACCTTGGG
  AGAGACAGCACGAAATTGCACTTGACAAATTTTACTGCAACTTACAAAAGACATCCCTCTTTGTCACCAA
  ATGGCAAAGCTCCTTGTTATGCTCCGTCTTCCCCTTTACCAGGAGATAGAAAGGTTTTCTCAGACAAAGC
  AGTTCTTCAGTCATGGACAGATAACGAGAGATTGGTTCCTAATGACGGTGCAGACTTTCCAGAGCACAGC
  TCCTATGGGAGAAATTCTCTGGAAGATAATTCCTGGGTATTCCCAAGCCCTCCTAAATCAAGTGAGACAG
  CATTTGGTGAAAATAAAAGCAAAATCTTACCTTTATCCAACCTGCCGCCACTGCATTACCTGGATCAACA
  GAATCAGAACTGCCTTTACAAAAGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286508
• Insert Size: 1218 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286508.1, NP_001273437.1
• RefSeq Size: 4549 bp
• RefSeq ORF: 1218 bp
• Locus ID: 27215
• UniProt ID: Q9QYP6
• Cytogenetics: 9 F3
• Gene Summary: Adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFNB promoter activation via TICAM1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. Variants 1 and 4 encode the same protein (isoform a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.