Hormad1 (NM_001289532) Mouse Untagged Clone

CAT#: MC227457

Hormad1 (untagged) - Mouse HORMA domain containing 1 (Hormad1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hormad1
• Synonyms: 4921522K05Rik; Nohma
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227457 representing NM_001289532
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACTATGCAGTTGCAGAGGACAGCTTCCCTGAGTGCATTGGTATTTCCCAATAAGATATCAACTG
  AGCATCAATCTTTGATGTTTGTGAAGAGGCTCCTAGCTGTTTCAGTATCTTGCATCACCTATTTGAGAGG
  AATATTTCCAGAACGTGCTTATGGGACAAGATATCTGGATGATCTCTGTGTCAAAATTCTGAAAGAAGAT
  AAAAATTGTCCAGGTTCTTCACAGCTAGTGAAGTGGATGCTTGGATGCTATGATGCTTTACAGAAGAAAT
  ATCTAAGGATGATCATTCTAGCTGTATACACCAATCCAGGAGATCCTCAGACAATTTCAGAATGTTACCA
  GTTTAAATTCAAGTACACCAAAAATGGACCAATCATGGACTTTATAAGCAAAAATCAAAACAATAAATCT
  AGTACAACATCTGCTGACACCAAGAAAGCAAGTATTCTCCTCATTCGGAAGATTTATGTCTTAATGCAAA
  ATCTAGGACCATTACCTAATGATGTTTGTCTGACCATGAAACTTTTTTACTATGATGAAGTTACACCCCC
  AGATTACCAACCACCAGGTTTTAAGGATGGTGACTGTGAAGGAGTAATATTTGATGGGGACCCTACATAC
  TTAAATGTGGGAGAAGTCCCAACACCTTTTCACACCTTCAGATTAAAAGTGACCACTGAGAAGGAACGAA
  TGGAAAATATTGATTCAACCATACTAAAACCAAAAGAATCAAAAACACAATTTGAAAAAATTCTAATGGA
  CAAAGATGATGTGGAAGATGAAAATCATAATAATTTTGACATTAAAACTAAAATGAACGAACAGAATGAA
  AACTCTGGAGCTTCTGAAATCAAAGAACCAAATTTAGATTGTAAGGAAGAAGAAACTATGCAATTCAAAA
  AGAGCCAAAGTCCTTCAATTTCTCATTGTCAGGTTGAACAGTTAGTCAGTAAAACATCTGAACTTGATGT
  GTCTGAAAGCAAAACAAGAAGCGGAAAAATCTTTCAGAGTAAAATGGTAAATGGAAATAATCAACAAGGA
  CAAACTTCTAAAGAAAATCGGAAGAGAAGTCTTCGTCAATTTAGGAAAACAGTCCTTCACGTCTTGGAAT
  CTAGTCAAGAGTCAGTGCTGAAGAAAAGGAGAGTTAGTGAACCAAAGGAACATACCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289532
• Insert Size: 1179 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289532.1, NP_001276461.1
• RefSeq Size: 1654 bp
• RefSeq ORF: 1179 bp
• Locus ID: 67981
• UniProt ID: Q9D5T7
• Cytogenetics: 3 F2.1
• Gene Summary: Plays a key role in meiotic progression (PubMed:19686734, PubMed:21079677, PubMed:21478856). Regulates 3 different functions during meiosis: ensures that sufficient numbers of processed DNA double-strand breaks (DSBs) are available for successful homology search by increasing the steady-state numbers of single-stranded DSB ends (PubMed:19686734, PubMed:21079677). Promotes synaptonemal-complex formation independently of its role in homology search (PubMed:19686734, PubMed:21079677). Plays a key role in the male mid-pachytene checkpoint and the female meiotic prophase checkpoint: required for efficient build-up of ATR activity on unsynapsed chromosome regions, a process believed to form the basis of meiotic silencing of unsynapsed chromatin (MSUC) and meiotic prophase quality control in both sexes (PubMed:21478856).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (a).