Bscl2 (NM_001290823) Mouse Untagged Clone

CAT#: MC227413

Bscl2 (untagged) - Mouse Berardinelli-Seip congenital lipodystrophy 2 homolog (seipin) (Bscl2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bscl2
• Synonyms: 2900097C17Rik; AI046355; Gng3lg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227413 representing NM_001290823
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAATGACCCACCAGTCCCCGCCTTACTGTGGGCCCAGGAAGTGGGCCACGTCTTGGCAGGCCGTG
  CCCGCAGGCTGATGCTGCAGTTTGGGGTGCTCTTCTGCACCATCCTTCTTTTGCTTTGGGTGTCTGTGTT
  CCTCTATGGCTCCTTCTACTACTCCTACATGCCGACGGTCAGCCACCTCAGCCCTGTGCACTTCCACTAC
  AGGACAGACTGTGATTCCTCCACCGCCTCTCTCTGCTCTTTCCCTGTTGCCAATGTCTCACTGGCTAAGA
  GTGGACGTGATCGGGTACTGATGTATGGACAGCCATATCGAGTCACCTTAGAGCTTGAACTGCCCGAATC
  CCCTGTGAATCAAGACTTGGGCATGTTCTTAGTCACTGTTTCATGTTATACCAGAGGTGGCCGAATCATC
  TCCACTTCTTCACGCTCAGTGATGCTGCATTACCGCTCGCAGCTGCTCCAGGTGCTCGACACGCTGCTGT
  TCTCCAGCCTCCTGCTGTTTGGCTTCGCTGAACAGAAGCAGCTCCTGGAAGTAGAACTCTACTCTGACTA
  TAGGGAGAATTCGTATGTGCCAACAACTGGAGCAATTATTGAGATCCACAGCAAGCGCATCCAGATGTAT
  GGAGCCTACCTCCGGATCCATGCCCACTTCACTGGGCTCAGGTACCTGCTGTACAACTTCCCCATGACCT
  GCGCCTTCGTGGGTGTGGCCAGCAACTTCACGTTCCTCAGCGTCATCGTGCTCTTCAGCTATATGCAGTG
  GGTGTGGGGTGCTGTCTGGCCCCGCCACCGCTTCTCTCTGCAGGTTAATATCCGACAAAGGGATAACTCA
  CACCACGGGGCCCCGCGTCGGATCTCGCGCCATCAGCCAGGTCAGGAATCTACCCAGCAGTCGGATGTGA
  CGGAGGATGGTGAGAGCCCTGAAGATCCCTCAGGAACAGAGGGCCAGCTGTCTGAGGAAGAGAAACCAGA
  GAAGCGGCCCCTGAATGGAGAGGAGGAGCAAGAGCCAGAGGCTAGTGATGGCTCCTGGGAAGATGCAGCT
  TTGCTGACAGAGGCCAACCCACCTACCTCTGCCTCAGCATCTGCCCTTGCCCCTGAGACTCTGGGTAGCC
  TCAGGCAACGCCCGACCTGCTCCAGTTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290823
• Insert Size: 1152 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290823.1, NP_001277752.1
• RefSeq Size: 1421 bp
• RefSeq ORF: 1152 bp
• Locus ID: 14705
• UniProt ID: Q9Z2E9
• Cytogenetics: 19 5.76 cM
• Gene Summary: Is a regulator of lipid catabolism essential for adipocyte differentiation. Necessary for correct lipid storage and lipid droplets maintenance; may play a tissue-autonomous role in controlling lipid storage in adipocytes and in preventing ectopic lipid droplet formation in non-adipose tissues. May also be involved in the central regulation of energy homeostasis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1.