Sept4 (NM_001284392) Mouse Untagged Clone

CAT#: MC227381

42251 (untagged) - Mouse septin 4 (Sept4), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sept4
• Synonyms: ARTS; Bh5; OTTMUSG00000001265; Pnutl2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227381 representing NM_001284392
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCATTCACTGGGATGGCAAGGGAACTCTGTCCCCGAGGACGGGACTGAAGCTGGGGATGACAAGG
  AGTATGTGGGCTTTGCAACCCTCCCCAATCAAGTCCACAGGAAGTCTGTGAAGAAAGGCTTTGACTTTAC
  ACTCATGGTGGCAGGAGAATCTGGTCTGGGTAAATCCACTCTTGTCAACAGCCTCTTCCTCACTGACTTG
  TACCGGGATCGGAAACTGCTGGGCGCCGAAGAGCGGATCATGCAAACCGTGGAGATTACTAAGCACGCAG
  TGGATATAGAAGAGAAGGGAGTGAGGCTGCGGCTCACCATTGTGGACACTCCAGGATTTGGGGATGCAGT
  CAACAACACAGAGTGCTGGAAGCCTGTGGCCGAATACATCGACCAGCAGTTTGAGCAGTACTTCCGAGAC
  GAGAGTGGCCTGAACCGCAAGAACATCCAGGACAACCGGGTGCACTGCTGCCTGTACTTCATCTCCCCGT
  TTGGCCACGGGCTCCGGCCATTGGATGTTGAATTCATGAAGGCCCTGCATCAGCGGGTCAACATTGTGCC
  TATCTTGGCTAAGGCGGACACACTGACGCCTCCTGAAGTGGACCGAAAGAAATGCAAAATCCGGGAGGAG
  ATCGAGCACTTTGGAATCAAGATCTATCAGTTCCCAGACTGTGATTCCGATGAGGACGAGGACTTCAAAT
  TACAGGACCAAGCCCTAAAGGAAAGCATCCCATTTGCGGTGATTGGCAGCAACACTGTGGTAGAAGCCAG
  GGGGCGGAGAGTTCGAGGCCGCCTCTACCCTTGGGGCATCGTGGAAGTGGAAAACCCAGGTCACTGCGAC
  TTTGTCAAGTTGAGGACGATGCTGGTGCGTACCCACATGCAGGACCTAAAGGATGTGACCCGAGAGACAC
  ACTACGAGAACTACAGGGCACAGTGTATCCAGAGCATGACCCGGCTAGTAGTGAAGGAACGGAATCGCAA
  CAAACTGACAAGAGAGAGTGGTACTGACTTCCCTATCCCTGCTGTCCCACCAGGGACAGATCCAGAAACT
  GAGAAGCTAATCCGGGAGAAAGACGAAGAGCTGCGGCGGATGCAGGAGATGTTACACAAAATCCAAAGAC
  AGATGAAGGAGACTCACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001284392
• Insert Size: 1140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001284392.1, NP_001271321.1
• RefSeq Size: 1426 bp
• RefSeq ORF: 1140 bp
• Locus ID: 18952
• UniProt ID: P28661
• Cytogenetics: 11 C
• Gene Summary: Filament-forming cytoskeletal GTPase. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.