Serpinb6a (NM_001243192) Mouse Untagged Clone

CAT#: MC227375

Serpinb6a (untagged) - Mouse serine (or cysteine) peptidase inhibitor, clade B, member 6a (Serpinb6a), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Serpinb6a
• Synonyms: 4930482L21Rik; D330015H01Rik; ovalbumin; PI-6; Serpinb6; Spi3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227375 representing NM_001243192
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCTCTACAGGAAGCAAATGGCACCTTTGCCTTAAACCTTTTGAAAATACTGGGTGAAGACAGCT
  CAAAAAATGTATTTTTGTCACCCATGAGCATATCCTCAGCCCTGGCTATGGTCTTCATGGGGGCAAAAGG
  AACCACTGCTAGCCAGATGGCTCAGGCACTTGCTTTGGATAAATGCAGCGGCAATGGAGGTGGAGATGTC
  CACCAGGGCTTCCAGTCCCTTCTCACCGAAGTGAACAAAACTGGCACACAGTACTTGCTCAGAACAGCCA
  ACAGGCTCTTCGGGGATAAGACTTGTGATCTTTTAGCGTCTTTTAAAGATTCCTGCCTCAAGTTCTATGA
  AGCAGAGTTGGAAGAGCTGGACTTTCAGGGTGCTACAGAGGAGTCCCGACAGCACATCAACACCTGGGTA
  GCCAAAAAGACAGAAGATAAAATCAAAGAGGTGCTGTCTCCAGGTACAGTGAATTCTGATACATCGCTAG
  TCCTTGTGAATGCCATCTACTTCAAAGGAAACTGGGAGAAGCAGTTTAACAAAGAGCATACCAGGGAGAT
  GCCATTCAAAGTCAGCAAGAATGAGGAGAAACCTGTGCAAATGATGTTTAAGAAGTCTACCTTTAAGATG
  ACCTATATTGGAGAGATATTCACTAAGATTCTGTTGCTTCCCTATGTCAGCAGTGAGCTGAACATGATCA
  TCATGCTTCCAGATGAGCACGTTGAACTGAGTACAGTGGAAAAGGAAGTAACTTACGAGAAATTTATAGA
  GTGGACAAGGCTGGACAAGATGGACGAAGAAGAGGTAGAAGTATTTCTCCCAAAGTTTAAGCTGGAGGAG
  AATTACAACATGAACGATGCCCTCTACAAGTTGGGCATGACTGATGCCTTTGGCGGCAGGGCAGACTTTT
  CTGGAATGTCTTCCAAGCAAGGCTTGTTTCTGTCTAAGGTTGTGCATAAGGCCTTTGTGGAGGTTAATGA
  GGAGGGCACAGAGGCTGCAGCTGCTACAGCTGGCATGATGACGGTGAGGTGCATGAGATTCACTCCCCGC
  TTCTGTGCCGACCACCCCTTCCTTTTCTTCATTCACCATGTTAAGACCAATGGAATTCTGTTCTGTGGCC
  GGTTCTCCTCTCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001243192
• Insert Size: 1137 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243192.1, NP_001230121.1
• RefSeq Size: 1504 bp
• RefSeq ORF: 1137 bp
• Locus ID: 20719
• UniProt ID: Q60854
• Cytogenetics: 13 14.0 cM
• Gene Summary: Inhibitor of cathepsin G, kallikrein-8 and thrombin. May play an important role in the inner ear in the protection against leakage of lysosomal content during stress. May be involved in the regulation of serine proteinases present in the brain or extravasated from the blood.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR and uses a downstream, in-frame start site, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a. Variants 2, 3 and 4 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.