Btbd8 (NM_001255991) Mouse Untagged Clone

CAT#: MC227368

Btbd8 (untagged) - Mouse BTB (POZ) domain containing 8 (Btbd8)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Btbd8
• Synonyms: EG627196; Gm16115; OTTMUSG00000030610
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227368 representing NM_001255991
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCACTGTGGAGCGGGAGGTGCGGCCCCGGTGGCCCTTCTGAGTTCCCCGGGGCATGGCAGGAAGG
  GGCTGCCCAGGAAGGGGCCCGGGGAGCGGCGCCGGCTGAAGGCGGCGGTGTCGGAGCAGCTCAGCCGCGA
  CGTGCTCAGGCTTTTAAGGGAGGAAATCCACACAGACACCATTCTTTCTGTCAGTGGCTCGCTGTTCAAA
  GTGCACAAGGCAGTCTTGTTAGCCAGAGCTCCTGGCTTTCATTCCCATATCATTGAACACACATCAAGTG
  ACCTAACAAATGAGCTTGTTCCTGTGGATGGTGTTGAAGCTTCAGAATTTAAAGCATTTTTACAGATTGT
  ATATTCATCAAACAAAAACATAAAAAACTATGAAGAGGAAATTGTTAAAAAACTGAAAGTGGGAAGCTTA
  ATGCCAGAAAAGGGGCCAGACGTCAGCTTCCCGCGGTATAGAACTTCGTCTGACTGTTTTCTTGGGAAAG
  GTGAAATTCCAGAGGATATTACTGGTGGAGGTGACTGCTTTATTTCCAAGGCTGATTCTGACCTGGAGCC
  TGCGTCTGAGTTAGGAGGAGATTTGTTGAAGCTTTACCTGAGTCAGCACTACCCTGACATTGACATTTGT
  GTTGATGGGAGGAGCTTCCGAGCCCACAGGGCCATTTTAAGTGCCAGGTCGAGTTATTTTGCTGCAATGC
  TGAGTGGCTGCTGGGCTGAAAGCTCCCAAGAGTGTATTACTCTTCAAGGAATAACCCATGTAGAAATGAA
  TGTTATGATGCACTTTATATATGGAGGAACTTTGGATTTTCCCGAAAAGGCTAATGTTGGTCAGATACTG
  AACGTGGCTGATATGTATGGACTGGAAGGATTGAGAGAAGTAGCAATCTATGTTTTAAGAAGAGATTACT
  GTAATTTCTTTCAGAAGCCTGTTCCCAGAACGTGGGCATCAATTCTAGAATGCTTAATCATTGCTCATTC
  AGTCGGAGTGGAAAGCCTTTTTGCTGACTGCATGAACTGCATTATAAGCCATTTTGCAAGGTTTTGGTCG
  GAAAGAAGCTTTGCAAATGTTCCTCCTGAGATTCAGAAAACCTGTCTCAGTATGCAGATTCAGTCCCTAG
  TGAGTATAGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001255991
• Insert Size: 1134 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001255991.1, NP_001242920.1
• RefSeq Size: 1134 bp
• RefSeq ORF: 1134 bp
• Locus ID: 100503185
• UniProt ID: D3YUB6
• Cytogenetics: 5 F
• Gene Summary: Involved in clathrin-mediated endocytosis at the synapse. Plays a role in neuronal development and in synaptic vesicle recycling in mature neurons, a process required for normal synaptic transmission.[UniProtKB/Swiss-Prot Function]