Nsfl1c (NM_001291074) Mouse Untagged Clone

CAT#: MC227324

Nsfl1c (untagged) - Mouse NSFL1 (p97) cofactor (p47) (Nsfl1c), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nsfl1c
• Synonyms: Munc-18c; p47; Stxbp3a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227324 representing NM_001291074
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGAGCGGCAGGACGCGTTGAGGGAGTTCGTGGCGGTGACTGGCACTGAGGAGGACAGGGCCC
  GTTTCTTCCTGGAGTCGGCTGGCTGGGACCTGCAGATTGCACTTGCAAGCTTTTATGAGGATGGAGGGGA
  TGAAGACATTGTGACCATTTCACAAGCAACCCCAAGTTCAGTGTCCAGAGGCACAGCTCCTAGTGATAAT
  AGAGTGACATCCTTCAGAGACCTCATTCATGACCAAGATGAAGAGGAGGAGGAAGAGGAGGGACAGAGGT
  TTTATGCTGGGGGCTCAGAGAGAAGTGGACAGCAGATTGTTGGTCCTCCCCGGAAGAAAAGTCCCAACGA
  GCTGGTGGATGATCTGTTTAAGGGTGCCAAAGAGCATGGGGCTGTAGCTGTGGAACGAGTGACCAAGAGC
  CCTGGAGAGACCAGTAAACCGAGACCATTTGCAGGAGGGGGCTACCGCCTTGGAGCAGCACCAGAGGAAG
  AGTCTGCCTATGTGGCAGGAGAAAGGAGGCGGCATTCAGGCCAGGATGTTCATGTAGTGCTAAAGCTCTG
  GAAAACTGGATTCAGCCTAGACAATGGTGACCTTAGAAGCTACCAAGACCCATCCAATGCCCAGTTTCTG
  GAGTCTATCCGTAGAGGGGAGGTTCCTGCAGAGCTTCGGAGGTTAGCGCATGGTGGGCAGGTGAACCTGG
  ATATGGAGGATCATCGGGATGAGGACTTCGTGAAGCCCAAGGGAGCTTTCAAAGCCTTCACTGGCGAGGG
  CCAGAAACTGGGCAGCACGGCCCCCCAGGTATTAAACACCAGCTCTCCAGCCCAACAAGCAGAAAATGAA
  GCCAAAGCTAGCTCGTCCATCTTAATCAATGAAGCAGAACCTACCACGAACATCCAAATCCGGCTTGCAG
  ATGGCGGGAGGCTGGTGCAGAAATTCAACCACAGCCACAGGATCAGCGACATCCGGCTCTTCATCGTGGA
  TGCCCGGCCAGCTATGGCTGCCACCAGTTTTGTCCTTATGACTACCTTCCCTAACAAAGAGCTGGCTGAT
  GAGAACCAAACCCTGAAGGAAGCCAACCTTCTCAACGCTGTCATCGTGCAGCGGTTAACATAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001291074
• Insert Size: 1113 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291074.1, NP_001278003.1
• RefSeq Size: 1480 bp
• RefSeq ORF: 1113 bp
• Locus ID: 386649
• UniProt ID: Q9CZ44
• Cytogenetics: 2 G3
• Gene Summary: Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Inhibits the activity of CTSL (in vitro). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase. Also, regulates spindle orientation during mitosis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.