Ppp2r2a (NM_001205188) Mouse Untagged Clone

CAT#: MC227310

Ppp2r2a (untagged) - Mouse protein phosphatase 2, regulatory subunit B, alpha (Ppp2r2a), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ppp2r2a
• Synonyms: 2410004D02Rik; mKIAA1541
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227310 representing NM_001205188
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGAGCTGGAGGAGGGAATGATATTCAGTGGTGTTTTTCTCAGGTGAAAGGAGCAGTAGATGATG
  ACGTAGCAGAAGCAGATATAATTTCTACAGTAGAGTTTAATCATTCTGGAGAATTACTAGCCACAGGAGA
  TAAAGGTGGGAGAGTTGTCATCTTTCAACAAGAGCAGGAGAACAAAATCCAGTCTCATAGCAGAGGAGAA
  TACAATGTTTACAGCACCTTCCAGAGCCATGAGCCAGAATTTGACTACTTGAAAAGCTTAGAAATAGAAG
  AGAAGATCAACAAAATTAGGTGGTTACCCCAGAAAAATGCTGCCCAGTTTTTATTGTCTACCAACGATAA
  AACAATAAAATTATGGAAAATCAGTGAAAGAGACAAAAGACCAGAAGGCTATAACTTGAAAGAGGAAGAT
  GGAAGATATAGAGATCCTACTACAGTCACTACGCTACGGGTGCCCGTATTTAGGCCCATGGATCTAATGG
  TGGAAGCCAGTCCACGGAGAATATTTGCCAACGCCCACACTTATCACATCAACTCAATTTCTATCAATAG
  TGATTATGAAACCTACTTATCAGCAGATGATTTGCGAATTAATCTCTGGCATCTGGAAATTACAGACAGG
  AGTTTTAATATTGTGGATATCAAGCCTGCCAATATGGAAGAGCTCACGGAGGTGATCACGGCTGCAGAAT
  TTCACCCCAACAGCTGTAACACGTTTGTGTACAGCAGCAGTAAAGGAACTATTCGGTTATGTGACATGAG
  GGCATCTGCGCTCTGTGACAGGCATTCTAAACTGTTTGAAGAACCTGAAGATCCCAGCAACAGGTCATTT
  TTTTCTGAAATCATCTCCTCTATTTCTGATGTAAAATTCAGCCATAGCGGTCGTTATATGATGACTAGAG
  ACTATTTGTCAGTCAAAATTTGGGACTTGAATATGGAGAACAGGCCCGTGGAGACATACCAGGTACATGA
  ATACCTCAGAAGTAAACTTTGCTCACTGTATGAAAACGACTGCATATTTGACAAATTTGAATGTTGTTGG
  AATGGGTCTGACAGGCACAGTAGACTGGAATTCCGTCTGGAAGTAACGTGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001205188
• Insert Size: 1104 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205188.1, NP_001192117.1
• RefSeq Size: 2663 bp
• RefSeq ORF: 1104 bp
• Locus ID: 71978
• UniProt ID: Q6P1F6
• Cytogenetics: 14 D1
• Gene Summary: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.