Ctdspl2 (NM_001290992) Mouse Untagged Clone

CAT#: MC227216

Ctdspl2 (untagged) - Mouse CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase like 2 (Ctdspl2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ctdspl2
• Synonyms: D2Ertd485e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227216 representing NM_001290992
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAATCAACATATAAAGCAAAATGGAAAATTAGAAGATAACCCTTGCTCTGGTAGTCCTCCGAGGA
  CTACATTGTTGGGGACCATATTTTCGCCTGTCTTCAACTTTTTTTCACCAGCAAATAAAAATGGAACGTC
  AGGCTCAGATTCCCCAGGACAGGCTGTGGAAGCTGAGGAGATAGTGAAACAACTTGACATGGAACAGGTG
  GATGAAATTACTACCAGCACCACGTCAGCTAATGGAGCGGCTTACTCAAATCAAGCAGTTCAAGTGAGGC
  CATCACTAAATAATGGTTTAGAAGAAGCAGAAGAAACAGTTACCCGGGATATCCCACCCCTTACAGCACC
  AGTAACTCCAGAGAGTGGGTACTCGTCAGCCCATGCAGAGGCCACCTATGAAGAAGACTGGGAAGTATTT
  GACCCCTATTATTTCATCAAACATGTTCCGCCTCTGACAGAAGAACAGCTAAATAGGAAACCTGCTCTTC
  CTTTGAAAACAAGAAGCACACCAGAGTTCTCCCTGGTCTTAGATCTGGATGAGACACTAGTGCACTGCAG
  TCTGAATGAGCTAGAAGATGCAGCGCTCACTTTTCCAGTCCTTTTCCAAGATGTCATTTATCAGGTTTAT
  GTGAGATTAAGACCATTCTTCAGGGAATTCCTGGAACGAATGTCTCAGATGTATGAGATCATTCTTTTTA
  CTGCTTCTAAGAAGGTGTATGCAGACAAGTTACTGAACATACTAGACCCTAAAAAGCAACTGGTCAGGCA
  CCGGCTTTTCCGTGAACATTGTGTTTGTGTACAAGGAAACTATATAAAGGACTTGAATATCCTTGGGAGA
  GATCTTTCCAAAACTATAATAATTGACAACTCACCACAAGCCTTTGCCTATCAGCTTTCTAATGGAATCC
  CTATAGAAAGCTGGTTTATGGATAAAAATGACAATGAACTCCTAAAATTGATTCCATTTCTGGAGAAGCT
  TGTAGAACTGAATGAAGATGTCCGGCCTCACATCAGAGACAGATTTCGATTGCATGATTTGCTGCCCCCA
  GATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290992
• Insert Size: 1056 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290992.1, NP_001277921.1
• RefSeq Size: 6733 bp
• RefSeq ORF: 1056 bp
• Locus ID: 329506
• UniProt ID: Q8BG15
• Cytogenetics: 2 60.46 cM
• Gene Summary: Probable phosphatase.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate splice site in the 5' region, and it thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (c) is shorter at the N-terminus, compared to isoform a.