Rbfox2 (NM_001286419) Mouse Untagged Clone

CAT#: MC227186

Rbfox2 (untagged) - Mouse RNA binding protein, fox-1 homolog (C. elegans) 2 (Rbfox2), transcript variant 9

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rbfox2
• Synonyms: Fbm2; Fxh; Hrnbp2; Rbm9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227186 representing NM_001286419
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAAAAGAAAATGGTAACTCAGGGTAACCAGGAGCCAACAACAACTCCTGACGCAATGGTTCAGC
  CTTTTACTACCATCCCGTTCCCACCACCTCCACAAAATGGAATTCCCACAGAATATGGAGTGCCACACAC
  TCAGGACTATGCCGGCCAGACCAGTGAACATAACCTGACCCTCTACGGGAGTACGCAGCCTCATGGAGAA
  CAGAGTAGCAATTCACCCAGCAACCAGAATGGATCTCTCACGACAGAAGGTGGAGCACAGACAGACGGAC
  AACAGTCACAGACACAAAGTAGTGAAAATTCAGAGAGTAAATCTACGCCCAAGCGACTACATGTCTCTAA
  TATTCCCTTCCGCTTCCGAGACCCTGACCTCCGGCAGATGTTTGGGCAGTTTGGCAAAATCCTAGATGTG
  GAAATAATCTTTAATGAGCGCGGCTCCAAGGTGAATAATGCAACAGCACGGGTCATGACCAACAAGAAGA
  TGGTCACGCCATATGCAAATGGCTGGAAGTTAAGCCCAGTAGTTGGAGCTGTGTATGGTCCTGAGTTATA
  TGCAGCATCCAGCTTTCAAGCTGATGTGTCCCTAGGCAATGAGGCGGCTGTGCCCTTGTCAGGAAGAGGA
  GGCATCAACACTTACATCCCTCTAATCATTCCTGGCTTCCCTTACCCAACTGCAGCCACCACGGCAGCTG
  CTTTCCGAGGAGCCCATCTGAGGGGCAGAGGGCGGACAGTGTATGGTGCAGTCCGAGCGGTACCTCCAAC
  AGCCATCCCCGCCTATCCAGGAATAGTGTTACAGGAACCAATCATTAGCGCTAAAATACCTCAGGGTGGA
  TATGCAGCCTACAGATATGCACAGCCTGCTACTGCAACCGCAGCCACAGCTGCTGCAGCCGCTGCAGCCG
  CTTACAGCGACGGTTACGGCAGGGTGTACACAGCTGACCCCTACCATGCCCTCGCCCCTGCCGCCAGCTA
  TGGAGTTGGCGCTGTGGCGAGTTTGTACCGAGGTGGCTACAGCCGATTTGCCCCCTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286419
• Insert Size: 1041 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286419.1, NP_001273348.1
• RefSeq Size: 6469 bp
• RefSeq ORF: 1041 bp
• Locus ID: 93686
• UniProt ID: Q8BP71
• Cytogenetics: 15 D3
• Gene Summary: RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis. Seems to act as a coregulatory factor of ER-alpha (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (9) has multiple differences in the 5' UTR and throughout the coding region, including initiation of translation from an alternate start codon, compared to variant 1. The encoded isoform (9) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.