Paqr7 (NM_001285849) Mouse Untagged Clone

CAT#: MC227174

Paqr7 (untagged) - Mouse progestin and adipoQ receptor family member VII (Paqr7), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Paqr7
• Synonyms: 2310021M12Rik; AV001994; BB007237; mPR; Mpra; PGLP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227174 representing NM_001285849
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGATGGCAGTAGCCCAGAAGTTCAACCACCTTCTGTCCAGCCTGTGGCACGTGGGCCAGAAGCCTC
  CGCAACCAGAACCTGTGTTTACAGTGGACCGGGCCCAGGTGCCGCCACTTTTCTGGAAGCCGTACATCTA
  TGCTGGCTACCGGCCGCTGCATCAGAACTGGTGTTTCTACTTCCGCACACTGTTTCAGCGGCACAACGAG
  GCTGTGAACGTGTGGACCCACCTCCTGGCGGCCCTGGCTCTGCTGCTGCGGCTGATCGGCTTGGCGGCAA
  GTGTGGACTTCCGGGAAGACCCTCACGCGCTGCCCCTCTTCTTCATCGTCTTGGCCTCCTTCACCTACCT
  CTCGTTCAGTGCTGTGGCTCACCTCCTGCAGGCCAAGTCGGAGTTCTGGCATTACAGCTTCTTCTTCTTG
  GACTATGTGGGTGTGGCCGTGTACCAGTTTGGCAGTGCCCTGGCACACTTCTACTATGCCATAGAGCCGT
  CCTGGCATGACAAGGTGCAGGCTATTTTCCTGCCCACGGCCGCCTTCCTGGCCTGGCTTTCCTGCGCTGG
  CTCCTGCTACAACAAGTACAGCCAGAAGCCGGGTCTGCTGGGGCGCATTTTCCAGGAGGCGCCATCGGCG
  CTGGCCTATGTGCTGGACATCAGTCCCGTGTTGCACCGCATCATAGTGTCTCCCCTCCCTGCCGAGGAGG
  ATCCCGCTCTTCTCTATCACAAATGCCAAGTGGTTTTCTTCCTTCTAGCTGCTGCCTTTTTCTCCACGGT
  TATGCCTGAGAGTTGGTTCCCCGGCAGCTGTCACATCTTTGGGCAGGGACACCAAGTTTTCCATGTCTTT
  TTGGTGCTGTGCACTCTGGCCCAGCTAGAGGCGGTGACACTGGATTATCAGGCCCGGAGGGGCATATACG
  AGCCTCTGCACGCCCGTTGGCCCCACAACTTCTCTGGCCTCTTCCTGCTCACCGTGGCCAGCAGCAGCCT
  CACTGCGCTCCTCCTCAGCCAGCTGGTGCGGCGCAAACTCCATCAGAAGACCAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285849
• Insert Size: 1038 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285849.1, NP_001272778.1
• RefSeq Size: 3695 bp
• RefSeq ORF: 1038 bp
• Locus ID: 71904
• UniProt ID: Q80ZE4
• Cytogenetics: 4 D3
• Gene Summary: Plasma membrane progesterone (P4) receptor coupled to G proteins. Seems to act through a G(i) mediated pathway. May be involved in oocyte maturation. Involved in neurosteroid inhibition of apoptosis. Also binds dehydroepiandrosterone (DHEA), pregnanolone, pregnenolone and allopregnanolone.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. All variants (1 through 5) encode the same protein.