Gmnc (NM_001285916) Mouse Untagged Clone

CAT#: MC227091

Gmnc (untagged) - Mouse geminin coiled-coil domain containing (Gmnc), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gmnc
• Synonyms: Gemc1; Gm606; Gm1778
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227091 representing NM_001285916
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAGCATTCTGCCTTGCCAAGATCAGTATTTTGTAGGAGGCCAGAGTTATAATTGCCCGTATTCCA
  CTACAACGTCAGAATCTGGTGTTGACGTTTCCAAGGAGACTTGGGTCTCTTTCTGGGCTGCTGGTCTCCT
  GGACAACACTGAGCTCCAGCAAGCACCACAAGTGCTGGAATCGCCCTCTGAGTTAAGTTTCCCTGTTCTG
  GACTCATGTTCATGGGAAGAGGCTCAGCTCTCATCTCAGCTCTACAGAAATAAACAGCTCCAGGATACTC
  TACTGCAGAAGGAGGAAGAACTTGCTAGGCTACATGAAGAGAATAATCACCTCAGACAATACCTGAATTC
  TACTTTGGTTAAACGTCTGGAAGAGAAGGCCAAGAAGTTGCTGTCATCAGATGAGTTTTCCAAAGTGTTT
  GGAAAACTCAGAAAAGAGAAGAGGAAACCCAAAGAACACAGACACTCCCCTGCTGAGATTCCCCAGTTCA
  AAACTGCCAAGAGAAACCTCTCCACAGAGTTTTCTAACTGTGAGGAACAACCTGGGCCCCATGTGGACCC
  CTGGGTTCTTCAAACCCTTGGGTTAAAAGACCTCAACACCATTGATGACACTCTACCAGCTAACTACAGT
  GCCACCACCTCTCATCCCAGAGCAGTTCCCAGTATTTCCTCTCTGTTTGTACATGATGCAAATGGCTATG
  CAAATGTCCCCAGAGATCTACACCTCGACTATGTAGGTCAGGGAACACATGGCTCACATACCACCTCCAT
  CCACCAGGAAGATTGTCACAGTTTTCCTAGGCTTTCTAATCCTCCAGTGAGGGTGCAGACTCTTCCTTAC
  CACACTGCAGATGTGTCACCCAACAAGACAGAGATGGCCTTTTCCACATCTTTGAGCCCTCACTGTAATG
  TGAAAACTCACTCCTTCCACCAGGGACAAGCCTTTGTGCGTCGAGATGAGGAAGGAGGTTGGAAGTTTAC
  CTGGGTCCCTAAGCAGACCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285916
• Insert Size: 1002 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285916.1, NP_001272845.1
• RefSeq Size: 4448 bp
• RefSeq ORF: 1002 bp
• Locus ID: 239789
• UniProt ID: Q3URY2
• Cytogenetics: 16 B2
• Gene Summary: Regulator of DNA replication. Promotes initiation of chromosomal DNA replication by mediating TOPBP1- and CDK2-dependent recruitment of CDC45L onto replication origins (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and contains an alternate 5' terminal exon compared to variant 1. The encoded protein (isoform 2) has a distinct N-terminus and is shorter than isoform 1.