Mest (NM_001252293) Mouse Untagged Clone

CAT#: MC227043

Mest (untagged) - Mouse mesoderm specific transcript (Mest), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mest
• Synonyms: AA408879; AI256745; Peg1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227043 representing NM_001252293
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGAGTGGTGGGTCCAAGTAGGGCTCCTGGCTGTGCCCTTGCTGGCTGCGTACCTGCACATCCCGC
  CCCCTCAGCTCTCCCCTGCTCTGCACTCATGGAAGACTTCTGGCAAGTTTTTCACCTACAAAGGCCTACG
  CATCTTCTACCAAGATTCTGTCGGTGTGGTCGGAAGCCCTGAGATAGTTGTGCTTTTACATGGCTTTCCA
  ACATCCAGCTATGACTGGTACAAGATCTGGGAAGGGCTGACCCTGAGGTTCCATCGAGTGATCGCCCTTG
  ATTTCTTAGGCTTTGGCTTCAGTGACAAGCCGAGACCACATCAGTACTCCATATTTGAGCAGGCCAGCAT
  CGTAGAGTCCCTTCTGCGGCACCTGGGGCTCCAGAACCGCAGAATCAACCTGCTGTCTCACGATTATGGA
  GATATCGTTGCTCAGGAACTGCTGTACAGGTACAAGCAGAATCGATCTGGCCGGCTCACCATAAAGAGTC
  TCTGTCTGTCGAATGGAGGTATCTTTCCTGAGACGCATCGTCCTCTCCTTCTCCAAAAGCTCCTCAAAGA
  CGGAGGTGTGCTTTCCCCCATCCTCACCAGGCTCATGAATTTCTTCGTGTTCTCTCGAGGTCTCACTCCA
  GTCTTTGGACCGTATACTCGACCCACTGAGAGTGAGCTGTGGGACATGTGGGCTGTGATTCGCAACAATG
  ACGGCAACCTGGTCATCGACAGTCTTTTACAGTACATTAATCAAAGGAAGAAATTCAGAAGACGCTGGGT
  GGGAGCGCTTGCTTCTGTGTCCATCCCCATTCATTTTATCTATGGGCCATTGGATCCTATAAATCCGTAT
  CCAGAGTTTTTGGAGCTGTACAGGAAAACGCTGCCGCGGTCCACAGTGTCGATTCTGGATGACCACATTA
  GCCACTACCCACAGCTAGAGGATCCCATGGGCTTCTTGAATGCATATATGGGCTTCATCAACTCCTTCTG
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252293
• Insert Size: 981 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252293.1, NP_001239222.1
• RefSeq Size: 2401 bp
• RefSeq ORF: 981 bp
• Locus ID: 17294
• UniProt ID: Q07646
• Cytogenetics: 6 12.53 cM