Nt5c3 (NM_001252374) Mouse Untagged Clone

CAT#: MC226882

Nt5c3 (untagged) - Mouse 5'-nucleotidase, cytosolic III (Nt5c3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nt5c3
• Synonyms: 1600024P05Rik; 2610206B05Rik; 3110004A18Rik; AI842776; cN-III; lupin; Nt5c3a; Nt5y; p36
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226882 representing NM_001252374
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTAATCAGGAGTCTGCTGTCCATTTGAAAATGATGCCAGAATTTCAGAAAAGCTCAGTTCGTATCA
  AGAACCCTACAAGAGTAGAAGAAATTATCTGTGGTCTCATTAAAGGAGGCGCTGCCAAACTTCAGATAAT
  AACAGACTTTGACATGACTCTAAGTAGGTTTTCCTACAATGGGAAAAGATGCCCAACGTGTCATAATATC
  ATTGACAACTGTAAGCTTGTTACAGATGAATGTCGAAGAAAGTTACTGCAGCTAAAGGAGCAGTATTATG
  CTATTGAAGTTGATCCTGTTCTCACCGTGGAAGAGAAGTTCCCTTACATGGTAGAGTGGTATACCAAATC
  TCACGGTTTGCTTATTGAACAAGGCATACCAAAAGCCAAACTTAAAGAGATTGTGGCTGACTCTGACGTT
  ATGCTCAAGGAAGGATATGAGAATTTCTTCGGTAAACTTCAACAGCACGGTATCCCTGTGTTCATATTTT
  CTGCTGGTATTGGTGATGTACTTGAAGAAGTTATCCGGCAGGCTGGAGTTTATCACTCAAACGTCAAAGT
  AGTGTCAAACTTCATGGATTTTGATGAAAATGGAGTGCTGAAAGGATTCAAAGGAGAGTTAATCCACGTG
  TTCAACAAACACGATGGTGCCCTGAAGAATACAGACTACTTCAGCCAGCTGAAAGACAACAGCAACATCA
  TTCTGCTCGGGGACTCCCAAGGAGACCTAAGAATGGCCGATGGCGTAGCCAATGTTGAACACATTCTGAA
  AATTGGTTATCTGAATGACAGAGTAGACGAGCTTTTAGAAAAATACATGGACTCTTATGATATTGTTTTA
  GTAAAAGAAGAATCATTGGAGGTTGTCAACTCAATTTTACAGAAGACATTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252374
• Insert Size: 894 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252374.1, NP_001239303.1
• RefSeq Size: 1530 bp
• RefSeq ORF: 894 bp
• Locus ID: 107569
• UniProt ID: Q9D020
• Cytogenetics: 6 B3
• Gene Summary: Nucleotidase which shows specific activity towards cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m(7)GMP). CMP seems to be the preferred substrate.[UniProtKB/Swiss-Prot Function]