D16Ertd472e (NM_001252438) Mouse Untagged Clone

CAT#: MC226818

D16Ertd472e (untagged) - Mouse DNA segment, Chr 16, ERATO Doi 472, expressed (D16Ertd472e), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: D16Ertd472e
• Synonyms: 1700010I10Rik; 2310009O17Rik; E330003K22Rik; Eurl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226818 representing NM_001252438
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGAAGAGGAGCAGTTTGTAAGCATTGACTTGAATGATGACAACATTTGCAGTGTTTGTAAACTGG
  GAACAGACAAAGACACGCTCTCCTTCTGCCACATTTGCTTCGAGCTAAATCTCGAGGGAGTGCCAAAGTC
  GAATCTTTTACACACCAAATCAGTGAGAGGCCATAAAGACTGCTTTGAAAAATACCATTTAATAGCAAAC
  CAGGATTGTTCTCGTTCCAAGCTTTCCAAAAGTACTTATGAAGGAGTTAAAACCATCGTGAGTAAGAAGA
  TAAACTGGATTGTGCAGTATGCCCAAAATAAAAATCTGGATTTGGAGTCCGAGTGTTCCAAAACTTCCCA
  GCATCCCCTGTTGAATTTCAGGCATAAGCCAGAGAAGAAATTGCTCCCACAGTTTGACTCTCAGGTGCCA
  AAGTACTCGGCAAAAGGCTCAGCCGGAAACGCAGGCAGCATCTCAAGCTATGCGCAGAGAATCCTGGAGC
  ACAGAGAAAATACAGACTTCCGGCTGGGTTTATTGGAAGACGCGGATGCATTGTGGACTCACAGTCACAG
  CCAGGCACAGAAAACAGAAGAGACAAGCTCTGGTCCGGAGGGGACTATCCAGACCCAGAATCCACATTAC
  AGCCGTGAAGAATTGAATTCGATGACTCTTGCTGAAGTAGTACAACTGAGTGCGAAGCTCCAACAGCGAA
  TCCAAGAGGTTTTTGAAGAGTTAACACACCAGGTGCAAGAGAAGGACTCTCTGGCGTCAGAGCTCCATGT
  CCGTCACGTGGCTATCGAGCAACTCCTCAAGAACTGCTCCAAGTTGCCATGCCTCCAAGTGGGACGGACA
  GGGACGAGGTCACACCTACCCATGAACCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252438
• Insert Size: 873 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252438.1, NP_001239367.1
• RefSeq Size: 6160 bp
• RefSeq ORF: 873 bp
• Locus ID: 67102
• UniProt ID: Q9D7G4
• Cytogenetics: 16 45.36 cM
• Gene Summary: Plays a role in cortical progenitor cell proliferation and differentiation (PubMed:27404227). Promotes dendritic spine development of post-migratory cortical projection neurons by modulating the beta-catenin signaling pathway (PubMed:27404227).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.