Nfam1 (NM_001271411) Mouse Untagged Clone

CAT#: MC226671

Nfam1 (untagged) - Mouse Nfat activating molecule with ITAM motif 1 (Nfam1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nfam1
• Synonyms: 4921501M20Rik; Cnaip
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226671 representing NM_001271411
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCTGGCTTCTGCGACGTGGGGCCCGAGTGCGATGCCTGCACCCACCCAGCTGGCTCCCAGCCT
  GGTGCTTCCTCTGTTTGCTGCCGGTGCCCCAGACCCTGCAGCTAACAGGATTGGTATCATTGACCCATAC
  CAGCCTTCCTATCATGGTGTCCCTGGCAAACACCGATGTGTTCTTCAGCTGCAGAATCGAGGACTTCACA
  AGACTTCAAAGAGATTTGCCTGTTAAGTTGTTTCATACGGATATCCATGGCCGGAGACGCTGGGAGAAGC
  AGATTAACTGCCAACACAGACCTGGCATGGAGAACCATACCAGGGATTGCATGGTTAAACTCAGCCAGGC
  AAACACATCAGCCACAGGCATTTACTACTTCATAGTCGAAGGAGAGGAAACATATCAGAGCGATGGCGTT
  GTCATTCTGGTCAGAGACACAGTGTACCAGCCACCTGCATTCAAGGTCCAGGAGGCTCTGATGCTTGGCT
  TTACCAGCCTGATGAGCGTCCTGGGTGTGCTGGGCACAGCGCTACTGCTTTGGAAGAAGAAACAGATATC
  AGTTCTGGGCAAGCACACTGCTAAGACTTGTTCTGGTCTCAAATCCACCGTCGGAACCACAAAGCCTCCA
  GCAGAATCTGTCTACACGTCCCTACAGCGTCGAGAGACGGAGGTCTACGCCTGTATGAAGGAAGAGACTG
  GAAGCCCTGTCTTCAGCCAAAGCCCAGCCACCAAGGAGAAGCTGAATAGATTTGAGGATGACAACGAATT
  TAACCTTGTCTATGAAAATCTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271411
• Insert Size: 795 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271411.1, NP_001258340.1
• RefSeq Size: 3962 bp
• RefSeq ORF: 795 bp
• Locus ID: 74039
• UniProt ID: Q8R4V1
• Cytogenetics: 15 E1
• Gene Summary: May function in immune system as a receptor which activates via the calcineurin/NFAT-signaling pathway the downstream cytokine gene promoters. Activates the transcription of IL-13 and TNF-alpha promoters (By similarity). May be involved in the regulation of B-cell, but not T-cell, development.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).