Emp1 (NM_001288627) Mouse Untagged Clone

CAT#: MC225988

Emp1 (untagged) - Mouse epithelial membrane protein 1 (Emp1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Emp1
• Synonyms: I-8-09; TMP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225988 representing NM_001288627
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGTGCTACTGGCTGGTCTCTTTGTGGTCCACATTGCCACTGCCATTATGCTGTTTGTCTCCACCA
  TTGCCAACGTCTGGATGGTTGCAGATTACGCAAATGCATCTGTAGGGCTTTGGAAGAACTGCACTGGTGG
  TAACTGCGACGGCTCCCTGTCCTACGGCAATGAAGATGCTATCAAGGCAGTGCAAGCCTTCATGATCCTC
  TCCATCATCTTCTCCATCATCTCCCTTGTGGTCTTCGTGTTCCAGCTCTTCACTATGGAGAAGGGAAACC
  GGTTCTTCCTCTCAGGGTCCACCATGCTGGTGTGCTGGCTGTGTATCCTGGTTGGAGTGTCAATCTACAC
  TCATCATTACGCCCACAGCGAAGGGAACTTCAACTCCAGCAGCCACCAAGGCTATTGTTTCATCCTGACC
  TGGATCTGCTTCTGTTTCAGCTTCATCATCGGCATACTCTATATGGTCCTGAGGAAGAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001288627
• Insert Size: 483 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001288627.1, NP_001275556.1
• RefSeq Size: 2826 bp
• RefSeq ORF: 483 bp
• Locus ID: 13730
• UniProt ID: P47801
• Cytogenetics: 6 66.25 cM