Etfbkmt (NM_001252097) Mouse Untagged Clone

CAT#: MC225788

Mettl20 (untagged) - Mouse methyltransferase like 20 (Mettl20), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Etfbkmt
• Synonyms: 4833442J19Rik; AI256744; ETFB-KMT; Mettl20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225788 representing NM_001252097
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGAGCATCAAAGATCTTGGCCAACGACATAGACCCCATTGCAGGAATGGCGATTACACTAAATT
  GCAAATTAAATGGACTGAATCCTTTTCCTGTTTTAACTAAAAACATTTTGAATACCCAACAAGGTAAGTT
  TGATCTCATTGTTCTTGGAGATATGTTTTATGATGAAGACCTTGCAGACAGTCTTCATCTGTGGCTGCAG
  AACTACTTTTGGACCCATGGAACCCGAGTACTGATTGGTGACCCTGGGAGGCCCCAGTTCAGTGGACATA
  GCATTCGGCATCAACTGTACCAACTAGTAGAATACACACTTCCAGAGCCAACTCAGCAAGAGAACAATGG
  ACTGACCACAAGTGCTGTATGGGATTTTCATCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252097
• Insert Size: 387 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252097.1, NP_001239026.1
• RefSeq Size: 1165 bp
• RefSeq ORF: 387 bp
• Locus ID: 320204
• Cytogenetics: 6 G3
• Gene Summary: Protein-lysine methyltransferase that selectively trimethylates the flavoprotein ETFB in mitochondria. Thereby, may negatively regulate the function of ETFB in electron transfer from Acyl-CoA dehydrogenases.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a.