Nipbl (NM_201232) Mouse Untagged Clone

CAT#: MC225390

Nipbl (untagged) - Mouse Nipped-B homolog (Drosophila) (Nipbl), transcript variant B, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nipbl
• Synonyms: Idn3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225390 representing NM_201232
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGGGATATGCCCCATGTCCCCATAACTACGCTTGCGGGGATTGCTAGTCTTACGGACCTCCTGA
  ACCAGCTGCCTCTCCCATCTCCTTTACCTGCTACAACTACAAAGAGCCTCCTCTTTAATTCACGAATAGC
  AGAAGAGGTGAACTGCCTTTTGGCCTGTAGGGATGACAATTTGGTTTCACAGCTTGTCCATAGTCTCAAC
  CAGGTATCAACAGATCACATAGAACTGAAAGATAACCTTGGCAGTGATGATCCCGAAGGCGATATACCCG
  TCTTGTTGCAGGCCGTCCTGGCAAGGAGTCCGAACGTTTTCAGGGAGAAAAGCATGCAGAACAGATATGT
  ACAGAGTGGAATGATGATGTCTCAGTATAAACTTTCTCAGAATTCCATGCACAGTAGTCCTGCATCTTCC
  AATTACCAACAAACCACTATCTCACATAGCCCCTCCAGCCGGTTTGTGCCACCACAGACAAGCTCTGGGA
  ACAGATTTATGCCACAACAAAATAGCCCAGTGCCTAGTCCATATGCCCCACAGAGTCCTGCAGGATACAT
  GCCATATTCCCATCCTTCAAGTTACACAACACATCCGCAGATGCAGCAAGCATCGGTATCAAGTCCTATT
  GTTGCCGGTGGCTTGAGAAACATCCATGATAACAAAGTTTCTGGTCCATTATCTGGCAACTCAGCTAACC
  ATCATGCTGATAACCCTAGACATGGCTCAAGTGACGACTACCTACACATGGTGCACAGGCTAAGTAGTGA
  TGATGGCGATTCTTCAACAATGAGGAATGCTGCATCTTTCCCCTTAAGATCTCCTCAACCAGTGTGTTCT
  CCTGCTGGAAGTGACGGAACACCTAAAGGCTCAAGACCACCTTTAATTCTACAGTCTCAGTCTTTACCTT
  GTTCATCACCTCGAGATGTTCCTCCAGATATCTTACTAGATTCTCCAGAAAGAAAACAAAAAAAGCAAAA
  GAAAATTAAATTAGGCAAGGATGAAAAAGACCAGAACGAGAAAGCTGCAATGTATGATATAATTAGCTCT
  CCAACCAAGGACTCCACTAAACTTACATTAAGACTTTCTCGTGTAAGATCTTCAGATATGGACCAGCAAG
  ACGATATGCTATCTGGTATGGAAAATAGCAATGTTTCAGAAAATGATATTCCTTTTAATGTGCAGTATCC
  AGGACAGACTTCAAAAACACCCATTACTCCACAGGACGTAAACCGCCCACTTAATGCTGCTCAGTGTTTG
  TCGCAGCAAGAACAAACAGCATTCCTTCCAGCAAATCAAGTGCCTGTTTTACAACAGAACACTTCAGTTG
  CTACAAAACAACCACAGACTTCTGTAGTACAGAATCAGCAGCAGGTATCACAACAGGGGCCGATATATGA
  TGAAGTCGAATTGGACGCGTTGGCAGAAATTGAACGGATAGAGCGAGAATCAGCCATTGAAAGGGAGCGC
  TTCTCAAAGGAAGTTCAAGATAAGGATAAGCCTTTGAAAAAAAGAAAACAAGATTCTTACCCACAGGAGG
  CTGGGGGTGCTACAGGAGGTAATAGACCAGCTTCTCAGGAGACGGGTTCTACGGGAAATGGGTCAAGGCC
  AGCATTAATGGTTAGCATTGATCTTCATCAGGCAGGAAGAGTGGACTCTCAGGCTTCTATAACTCAGGAT
  TCAGACTCCATAAAAAAGCCTGAAGAAACCAAACAGTGTAATGATGCGCCCATTTCTGTTCTTCAGGAAG
  ATATTGTTGGAAGTCTTAAGTCTATACCAGAAAACCATCCTGAGACTCCTAAAAAAAAGTCTGATCCTGA
  GCTTTCAAAAAGCGAAATGAAACAAAATGAAAGTAGGTTGTCAGAATCTAAACCAAATGAAAATCAGCTG
  GGGGAGTCAAAATCAAATGAAAGTAAGTTAGAAACTAAAACTGAGACCCCAACAGAAGAACTTAAGCAGA
  ACGAGAACAAAACGACAGAGTCCAAACAGAGCGAGAGCGCTGTAGTTGAGCCTAAACAGAATGAAAATAG
  ACCGTGTGACACAAAACCAAATGACAACAAACAGAACAACACCAGGTCAGAAAACACAAAAGCAAGACCT
  GAAACCCCAAAGCAAAAAGCTGAAAGCCGGCCTGAAACCCCAAAGCAAAAGAGTGAAGGCCGGCCTGAAA
  CCCCAAAACAGAAGGGTGATGGACGACCTGAGACTCCAAAGCAAAAGAGTGAAGGCCGGCCTGAAACTCC
  AAAGCAAAAAGGTGAAGGCCGGCCTGAAACTCCAAAACATAGGCATGAAAATAGGAGGGATTCTGGAAAG
  CCATCAACAGAGAAGAAACCCGATGTGTCTAAGCATAAACAGGATATCAAATCTGACTCACCTCGATTAA
  AATCAGAAAGAGCTGAAGCCTTAAAGCAGAGACCTGATGGGCGATGGGAGTCACTAAGACGTGACCATGA
  TAGTAAACAGAAATCAGATGACAGGGGTGAGTCAGAGCGACATCGGGGTGATCAGTCAAGGGTTCGAAGA
  CCAGAAACATTGAGATCTTCTAGTAGAAATGACCATAGCACTAAATCAGATGGTTCAAAAACTGAAAAGC
  TAGAAAGAAAACACAGGCATGAATCAGGGGACTCACGGGACAGACCTTCTGGGGAACAAAAATCAAGACC
  AGACAGTCCTCGTGTAAAACAAGGAGATACTAATAAATCAAGACCTGGTTTTAAGTCACCAAATAGTAAA
  GATGACAAAAGGACAGAGGGTAACAGAAGTAAAGTAGACAGTAATAAAGCGCACACTGATAATAAGGCCG
  AATTTCCAAGCTATTTGTTGGGGGGGAGGTCTGGTGCATTGAAAAATTTCGTCATTCCAAAAATCAAGAG
  GGATAAAGATGGCAACATAACTCAGGAAACTAAGAAAATGGACATGAAAGGAGAGCAGAAAGACAAAGTA
  GAAAAAATGGGATTAGTTGAAGATCTAAACAAAGGAGCTAAGCCTGTAGTTGTTCTGCAAAAACTATCGC
  TGGATGATGTCCAGAAGCTAATTAAAGATAGAGAGGAGAAATCAAGAAGTTCCCTTAAGTCTATCAAGAA
  TAAGCCATCAAAGTCAAATAAAGGTAGTATAGATCAATCAGTGTTAAAAGAATTGCCCCCTGAGCTCCTG
  GCAGAAATTGAATCTACCATGCCACTTTGTGAACGTGTGAAAATGAACAAACGCAAGCGTAGCACAGTTA
  ATGAAAAGCCAAAATATGCTGAAATCAGTTCAGATGAAGATAATGATAGTGATGAAGCGTTTGAATCCTC
  TAGGAAACGGCATAAAAAAGATGACGATAAAGCTTGGGAATACGAAGAGCGCGACAGAAGAAGCTCTGGG
  GATCATAGGAGAAGTGGCCACTCCCATGACGGAAGGAGGAGCTCGGGTGGTGGTCGTTACCGAAACCGAA
  GTCCGTCCGATTCTGACATGGAAGATTACTCCCCTCCTCCCAGCCTTAGTGAGGTTGCTAGAAAAATGAA
  GAAAAAAGAAAAACAAAAGAAAAGGAAAGCTTACGAGCCAAAGCTAACGCCCGAAGAAATGATGGATTCT
  TCAACTTTTAAGAGATTTACAGCCTCAATAGAGAATATTTTGGATAATTTAGAAGATATGGATTTTACTG
  CATTTGGTGATGACGATGAGATTCCTCAGGAACTGCTTTTAGGAAAACATCAGCTTAATGAACTTGGGAG
  TGAATCTGCTAAAATTAAAGCCATGGGTATAATGGACAAGCTTTCAACTGACAAAACTGTGAAAGTCCTA
  AATATATTGGAGAAGAATATTCAGGATGGATCAAAGCTTTCCACTTTGTTAAATCATAATAATGATACTG
  AAGAAGAAGAAAGATTGTGGAGAGACCTAATTATGGAAAGAGTTACAAAGTCAGCAGATGCCTGTCTTAC
  AACTATCAACATTATGACATCTCCTAACATGCCAAAAGCTGTATACATTGAAGATGTAATTGAAAGGGTT
  ATACAGTACACTAAGTTTCATTTACAGAACACACTCTATCCCCAGTATGACCCTGTTTACAGAGTAGATC
  CACATGGAGGAGGGTTATTAAGTTCAAAGGCCAAACGGGCTAAATGTTCTACTCATAAGCAGAGAGTAAT
  TGTAATGCTTTACAACAAAGTTTGTGACATTGTTAGCAGTTTATCAGAATTGCTAGAGATACAGCTTCTT
  ACAGACACAACAATTCTTCAGGTTTCATCAATGGGAATAACACCCTTTTTTGTGGAAAATGTCAGTGAAC
  TACAATTGTGTGCCATTAAGTTAGTCACTGCAGTATTTTCAAGATATGAAAAGCATAGGCAGTTAATATT
  GGAAGAAATTTTTACTTCACTTGCAAGATTACCAACTAGCAAGAGAAGTTTAAGGAATTTCAGGTTAAAT
  AGTAGTGATGTGGATGGAGAACCTATGTATATTCAGATGGTTACAGCTCTAGTTTTACAACTTATTCAGT
  GCGTAGTTCACTTACCATCATCGGAGAAGGATCCTAATTCAGAAGAAGACTCAAACAAAAAAGTTGATCA
  GGATGTTGTCATTACGAACTCTTACGAAACAGCTATGAGAACAGCCCAAAACTTTCTCTCCATCTTTCTT
  AAAAAATGTGGTAGTAAACAAGGAGAAGAAGATTACAGACCGCTTTTTGAAAACTTTGTTCAAGACCTTC
  TCTCAACAGTCAATAAGCCCGAGTGGCCGGCTGCTGAACTACTCCTTAGCTTGTTAGGGAGGCTGTTGGT
  TCATCAGTTCAGTAACAAGTCAACAGAAATGGCTTTAAGAGTGGCATCTCTTGATTACCTTGGGACTGTT
  GCTGCGCGACTGAGAAAAGATGCTGTCACCAGCAAGATGGATCAAGGGTCTATAGAACGAATTTTAAAAC
  AGGTTTCTGGAGGAGAAGATGAGATCCAACAATTGCAAAAAGCATTGCTTGATTATCTGGATGAGAACAC
  TGAAACTGACCCTTCGCTAGTGTTTTCTCGTAAATTCTATATAGCCCAGTGGTTCCGTGACACAACTCTG
  GAAACAGAAAAAGCAATGAAATCACAAAAAGATGAAGAATCATCTGACGCAACACATCATGCAAAAGAAC
  TTGAGACAACTGGGCAGATCATGCATCGCGCTGAGAACCGGAAAAAGTTTCTTCGAAGCATTATCAAAAC
  CACACCTTCTCAGTTCAGCACATTAAAGATGAACTCTGACACTGTGGACTATGATGATGCGTGCTTGATT
  GTTCGATATTTGGCCTCTATGAGGCCGTTTGCCCAGAGCTTTGATATTTATTTGACACAGATACTACGAG
  TTCTTGGTGAAAATGCAATTGCCGTTCGAACAAAAGCCATGAAGTGTTTGTCTGAGGTTGTTGCTGTAGA
  CCCCAGTATTCTAGCAAGGCTTGACATGCAGCGCGGAGTTCATGGGCGACTAATGGATAACTCCACTAGT
  GTTCGAGAGGCAGCAGTGGAATTGCTAGGTCGCTTTGTCCTGTGTCGCCCTCAGCTTGCTGAGCAGTATT
  ATGACATGCTGATTGAAAGAATATTGGATACTGGGATCAGTGTCAGGAAAAGAGTAATAAAGATTTTAAG
  AGACATCTGTATTGAACAACCAACATTTCCAAAAATTACAGAAATGTGTGTAAAAATGATTCGTAGAGTC
  AATGATGAAGAGGGCATTAAGAAATTAGTAAATGAAACATTCCAAAAGCTCTGGTTTACTCCAACTCCAC
  ATAATGACAAAGAAGCAATGACAAGGAAGATTTTAAACATCACTGATGTGGTTGCAGCATGTAGAGATAC
  TGGATATGACTGGTTTGAGCAACTCCTTCAAAATTTGTTGAAGTCTGAAGAGGATTCCTCATATAAACCT
  GTGAAGAAAGCTTGTACTCAACTTGTTGATAACCTAGTTGAACATATTCTTAAATATGAGGAATCTCTAG
  CTGACTCAGACAATAAAGGTGTAAATTCTGGAAGATTGGTAGCTTGTATAACCACTTTGTTCTTATTCAG
  CAAAATAAGACCCCAACTCATGGTTAAACATGCCATGACTATGCAGCCGTACCTCACCACTAAGTGTAGT
  ACGCAAAATGATTTCATGGTTATCTGCAATGTTGCAAAAATCCTTGAGTTAGTTGTACCACTGATGGAAC
  ACCCAAGTGAAACTTTCCTTGCCACTATTGAGGAAGATCTGATGAAGCTGATCATCAAATACGGCATGAC
  TGTAGTACAACATTGTGTGAGCTGTCTTGGGGCTGTTGTAAATAAAGTGACCCAGAATTTTAAATTTGTG
  TGGGCCTGTTTCAATAGATACTATGGTGCCATTTCAAAATTAAAAAGTCAGCACCAAGAAGACCCAAATA
  ACACTTCACTTTTAACAAATAAACCAGCGCTTCTTAGGTCCCTCTTCACTGTTGGAGCACTTTGCCGCCA
  TTTTGATTTTGATCTGGAAGATTTTAAAGGCAACAGTAAGGTTAACATAAAGGATAAAGTACTTGAACTG
  TTGATGTATTTTACAAAACACTCAGATGAAGAAGTACAAACAAAAGCCATCATTGGTCTAGGATTTGCCT
  TTATACAGCATCCGAGTCTAATGTTTGAGCAAGAAGTGAAGAATCTGTATAATAGTATTTTATCTGATAA
  GAACTCCTCAGTGAATTTAAAAATACAAGTGTTAAAAAACCTCCAGACTTACCTACAAGAAGAAGACACG
  CGAATGCAACAAGCAGACAGAGACTGGAAGAAAGTTGCAAAACAGGAAGACCTGAAGGAAATGGGGGACG
  TCTCCTCGGGGATGAGCAGCTCCATCATGCAGCTGTACCTCAAGCAGGTGCTGGAGGCGTTCTTTCATAC
  CCAGTCAAGTGTGCGCCACTTCGCCCTGAATGTCATTGCTCTGACTCTGAACCAAGGCCTCATCCATCCG
  GTTCAGTGTGTTCCATACTTAATTGCCATGGGAACAGATCCAGAACCTGCTATGCGAAACAAGGCTGATC
  AGCAGCTTGTGGAGATAGACAAAAAATATGCTGGGTTCATCCATATGAAAGCCGTGGCTGGTATGAAGAT
  GTCTTACCAGGTCCAACAGGCGATCAACACGTGCCTAAAAGACCCTGTAAGGGGTTTCCGACAAGATGAA
  TCCTCCAGTGCTCTGTGCTCACATCTATACTCCATGATCCGCGGGAACCGCCAACACAGACGCGCCTTTC
  TTATTTCTTTACTCAACCTCTTTGATGACACAGCAAAAACAGAAGTGACTATGCTCTTGTACATAGCAGA
  CAATCTAGCCTGTTTTCCATACCAGACTCAGGAAGAGCCGCTGTTTATAATGCACCACATAGACATTACG
  CTCTCTGTTTCCGGTAGTAACCTATTGCAGTCATTCAAGGAGTCTATGGTAAAAGACAAAAGGAAAGAGA
  GGAAAACGTCCCCTGCTAAAGAGAACGAGTCCAGTGAGAGTGAAGAAGAAGTGTCCAGACCTCGGAAGTC
  TCGAAAGCGGGTAGATTCGGAGTCAGACTCAGACTCTGAAGATGATATAAATTCAGTGATGAAGTGTTTG
  CCGGAAAATTCAGCCCCTTTGATTGAATTCGCAAATGTTTCCCAGGGTATTTTATTACTTCTTATGTTAA
  AGCAGCACCTGAAGAATCTCTGTGGATTTTCTGATAGTAAAATTCAAAAATATTCTCCATCTGAATCTGC
  AAAAGTGTATGATAAAGCCATTAATCGGAAAACAGGGGTTCATTTTCATCCCAAACAAACACTGGACTTC
  CTGCGAAGTGACATGGCTAATTCCAAACTGACGGAGGATGTGAAGAGGAGCATCGTAAGGCAGTACTTAG
  ACTTTAAACTTCTCATGGAACATCTAGACCCTGATGAAGAAGAAGAGGAAGGAGAGGTGTCAGCTAGCAC
  CAATGCTCGGAACAAAGCAATTACCTCGCTGCTTGGAGGAGGCAGCCCTAAAAATAACACAGCAGCAGAC
  ACAGAAGATGAGGAGAGTGATGGGGAGGATAGAGGAGGAGGCACTTCAGGGGTGAGGCGGAGGAGGAGTC
  AACGTATTTCCCAGCGTATTACGTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_201232
• Insert Size: 8076 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201232.2, NP_957684.1
• RefSeq Size: 10762 bp
• RefSeq ORF: 8076 bp
• Locus ID: 71175
• UniProt ID: Q6KCD5
• Cytogenetics: 15 A1
• Gene Summary: Plays an important role in the loading of the cohesin complex on to DNA (PubMed:29094699). Forms a heterodimeric complex (also known as cohesin loading complex) with MAU2/SCC4 which mediates the loading of the cohesin complex onto chromatin. Plays a role in cohesin loading at sites of DNA damage. Its recruitement to double-strand breaks (DSBs) sites occurs in a CBX3-, RNF8- and RNF168-dependent manner whereas its recruitement to UV irradiation-induced DNA damage sites occurs in a ATM-, ATR-, RNF8- and RNF168-dependent manner (By similarity). Along with ZNF609, promotes cortical neuron migration during brain development by regulating the transcription of crucial genes in this process. Preferentially binds promoters containing paused RNA polymerase II. Up-regulates the expression of SEMA3A, NRP1, PLXND1 and GABBR2 genes, among others (PubMed:28041881).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (b) differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) has a shorter and distinct C-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.