Cep250 (NM_001130000) Mouse Untagged Clone

CAT#: MC225312

Cep250 (untagged) - Mouse centrosomal protein 250 (Cep250), transcript variant 4, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cep250
• Synonyms: AW490617; B230210E21Rik; Cep2; Inmp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225312 representing NM_001130000
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACAGGGAGCCCCGGGTTGAACATGAAGCCCCAGTCCTTGCAGCTGGTGCTGGAGGGGCAGGTGC
  TGGCGCTGCAGCAGCAGATGGCCGAGAATCAGGCTGCCTCCTGGCGGAAACTGAAGAACTCCCAAGAGGC
  CCAGAAGAGACAAGCAACCCTCGTGAGGAAGCTGCAGGCCAAGGTTTTACAGTACCGGAGCTGGTGTCAA
  GACCTGGAGAAGCGACTAGAAGCCACTGGGGGGCTGATCCCTCAGCGCTGGGAGAGTGTAGAAGAGCCCA
  ACCTGGAACAGTTGCTGATCCGATTGGAGGAGGAGCAGCAGAGGTGTGAGAGTCTAGTGGAGGTGAACAC
  CGAGCTTCGGCTGCACATGGAGAAAGCCGATGTGGTGAATAAAGCCTTGCAGGAGGACGTGGAAAAACTG
  ACAGTGGACTGGAGTCGGGCCCGAGATGAGTTAGTGAGGAAGGAGAGCCAGTGGCGGATGGAGCAGGAGT
  TCTTCAAGGGCTATTTGAGAGGTGAACACGGTCGTCTTCTCAATCTATGGCGAGAGGTGGTAACCTTCCG
  ACGTCACTTCCTGAAAATGAAGTCAGCTACTGACAGAGACCTGACAGAGCTAAAGGCTGAACACGCAAGG
  CTTTCAGGGTCCCTGCTGACCTGCTGTCTGCGCCTGACCCTGAGAGCACAGTCTCGGGAGTCCAGTGGAT
  CTGGGAGAACAGAGGAGAGTGAGCCAGCCCGGCTGCTGCTGCTCGTCGCTAAGACCCAGGCGCTGGAGAA
  GGAAGCCCATGAGAAGAGCCAGGAGTTAATGCAGCTCAAGAGTCACGGGGATCTAGAGAAGGCAGAGCTG
  CAGGACCGAGTGACGGAACTCTCTGCTCTGCTAACCCAGTCTCAGAAGCAAAATGAAGATTATGAGAAGA
  TGGTAAAGGCTCTAAGAGAAACAATGGAGATCCTGGAGACAAACCATGCAGAGTTAATGGAGCATGAGGC
  ATCTCTCAGTAGGAATGCTCAAGAGGAAAAGTTGTCTCTGCAGCAGGTGATCAAGGCTATAACCCAGGCA
  CTGGCCTCAGTGGAAGAAGAAGACACTGTGACCCAGTCCTCAGGCCATGAGGACTCGCTGCAGTCAGACT
  GTAATGGCCTTTCCCAGTTTGATCCCCAGGACCCAGACAGGGCTCTTACCCTGGTGCAGTCAGTGCTGAC
  TCGGAGACAGCAGGCTGTGCAGGACCTCAGGCAGCAGCTTTCAGGCTGCCAGGAGGCTATGAGCTTCTTG
  CAGCAGCAGCACGATCAGTGGGAGGAAGAGGGCAGGGCCCTGAGGGAAAAGCTACAGAAGCTCACTGGGG
  AGCGGGATGCTCTGGCAGGCCAGACTGTGGGCCTTCAGGGAGAGGTGGACTCTCTCACTGGGGAGCGGGA
  TGCTCTGGCAGGCCAGACTGTGGACCTTCAGGGAGAGGTGGACTCTCTCAGCAGGGAGCGGGAGCTGCTT
  CAGAAGGCCAGAGGAGAGCTGCAGCAGCAACTGGAAGTGCTGGAGCAGGAAGCGTGGCGACTGCGGAGGA
  TGAACATGGAGCTGCAGCTGCAGGGGGACTCAGCTCAAGGGGAGAAGCTGGAGCAGCAGGAGGAGTTGCA
  CTTGGCTGTCCGGGAGAGGGAGCGCCTTCAGGAGACGCTGGTGGGCCTGGAAGCCAAGCAGTCAGAATCA
  CTAAGTGAGCTGCTCACTCTTCGAGAAGCCCTGGAGTCAAGTCGCCTAGAAGGGGAGTTACTGAAGCAGG
  AGCGAGTGGAAGTGGCTGCAGCCTTGGCCAGGGCAGAACAGTCCATTGTGGAGCTGTCAGGTTCTGAGAA
  CAGCCTGAAGGCAGAGGTAGCTGATCTTCGAGCTGCAGCTGTCAAGCTTGGGGCCTTAAACGAGGCTTTG
  GCTTTAGATAAAGTTGAACTGAACCAGCAGCTTCTCCAGTTGGAGCAGGAGAACCAGTCTCTATGCAGCA
  GAGTGGAGGCCGCAGAGCAGCTGAGAAGCGCTTTGCAGGTGGACCTGGCGGAGGCAGAAAGGCGCAGGGA
  AGCCCTGTGGGAGAAGAAAACTCAGCTGGAGACTCAGCTGCAGAAGGCAGAGGAAGCGGGGGCTGAGCTA
  CAGGCAGAGCTGAGGGGCACCCGAGAAGAAAAGGAAGAGCTTAAAGATAAATTAAGTGAGGCACATCACC
  AGCAAGAAACAGCTACAGCTCACCTAGAACAGCTGCATCAGGACGCAGAAAGACAGGAAGAAACACTTGC
  TAGGGCAGTCCAGGAGAAGGAAGCCCTAGTACGGGAGAGGGCAGCTCTTGAGGTGCGCCTGCAGGCTGTG
  GAGCGGGACCGACAGGACCTCACTGAACATGTCCTGGGGCTCAGGTCAGCCAAGGAGCAACTGGAGAGCA
  ATCTATTTGAGGCCCAACAGCAAAATTCTGTGATACAGGTCACCAAGGGGCAGCTGGAGGTCCAGATTCA
  AACTATTATCCAAGCCAAGGAAGTAATTCAAGGGGAAGTGAAATGCCTGAAGCTGGAACTGGATGCTGAG
  AGGACCCGGGCAGAGCAGGAGTGGGATGCAGTAGCCAGGCAGCTAGCCCAAGCTGAGCAGGAGGGGCAAG
  CATCTCTGGAGCGACAGAAGGTGGCCCACGAGGAGGAGGTGAACAGGCTGCAAGAGAAATGGGAGAAAGA
  GCGCTCCTGGCTGCAGCAGGAGCTGGATAAGACCCTGGAAACCCTCGAGAGGGAGAGAGCAGAGCTGGAG
  ACAAAGCTGAGAGAGCAGCAGACAGAAATGGAAGCCATCCGTGCACAGAGGGAAGAGGAACGAAGCCAGG
  CTGACAGCGCCCTGTATCAAATGCAGCTAGAAACAGAGAAGGAGAGAGTATCCCTCCTGGAGACACTGCT
  GAGGACCCAGAAGGAGCTGGCAGATGCTAGTCAACAGCTGGAAAGGCTGAGACAGGACATGAAGATTCAG
  AAGTTAAAGGAACAGGAGACCACTGGCATGCTGCAGGCCCAGCTTCAGGAAACACAGCAGGAGCTGAAGG
  AGGCAGCCCAGCAGCACAGGGACGACCTTGCTGCCTTCCAGAAGGACAAGCTGGACCTGCAGAAGCAGGT
  AGAGGACCTAATGTCTCAGCTGGTAGCCCACGATGACTCCCAGAGGCTGGTGAAAGAAGAGATTGAAGAG
  AAGGTGAAAGTGGCTCAAGAATGCAGCCGGATTCAGAAGGAACTGGAGAAAGAGAACGCCAGCCTGGCTC
  TGTCGCTGGTGGAAAAGGAAAAACGACTTCTCATTTTACAAGAAGCTGACTCTGTAAGACAGCAGGAGCT
  GAGCTCCTTGCGCCAGGACATACAGGAGGCCCAGGAAGGGCAAAGAGAGCTGGGTGTACAGGTGGAACTA
  TTGAGGCAGGAGGTGAAGGAAAAGGAGGCTGACTTTGTGGCCCGGGAAGCACAGCTGCTGGAGGAGTTGG
  AGGCCTCACGGGTTGCAGAGCAGCAACTTCGAGCGTCTTTGTGGGCCCAGGAAGCCAAGGCAACCCAGCT
  GCAGCTGCAGCTGCGCAGCACTGAGAGCCAGCTAGAGGCATTGGTTGCAGAACAACAGCCGGAGAACCAG
  GCACAGGCACAGCTAGCTAGCCTCTGCTCTGTTCTGCAGCAGGCCCTGGGGTCTGCATGTGAGAGTAGGC
  CTGAGCTGAGGGGTGGAGGCGACTCTGCTCCTACCTTATGGGGCCCTGATCCAGATCAGAATGGAGCTTC
  CAGACTCTTTAAGAGATGGTCGCTCCCAACGGCACTGTCACCGGAGGCTGTGGCACTAGCTCTCCAGAAG
  CTCCATCAGGACGTGTGGAAGGCTCGGCAGGCCCGGGATGACCTAAGGGACCAGGTCCAGAAGCTGGTGC
  AGCGCCTGACTGACACGGAGGCCCAGAAGAGCCAGGTTCACTCGGAGCTGCAGGACCTGCAGAGACAGCT
  CTCTCAGAGCCAGGAAGAGAAATCCAAGTGGGAAGGGAGGCAGAACTCCCTGGAGTCTGAGCTGAGGGAC
  TTGCACGAGACCGCGGCATCCTTACAGAGTCGCCTCCGGCAGGCAGAACTGCAAAAAATGGAAGCTCAGA
  ATGATCGAGAGTTGCTTCAGGCATCCAAGGAGAAGCTGTCAGCCCAGGTTGAACATCTGCAAGCATGTGT
  TGCAGAAGCCCAGGCTCAGGCAGATGCAGCTGCTGTCCTGGAAGAAGATCTGCGAACTGCTCGCTCTGCA
  CTGAAACTGAAAAACGAGGAGCTAGAGAGTGAACGGGAGAGAGCCCAGGCTTTACAAGAACAGGGTGAGC
  TGAAGGTGGCTCAGGGGAAAGCTTTGCAGGAGAACCTAGCCCTGCTGGCACAGACCCTGTCTAACAGAGA
  GCGGGAGGTCGAGACGTTGCAGGCTGAGGTTCAGGAACTGGAGAAGCAGCGGGAGATGCAGAAGGCTGCT
  CTGGAGCTGCTCTCCCTCGACCTGAAGAAGCGCAGTCGGGAGGTAGACCTGCAACAAGAACAGATTCAGG
  AGCTGGAGCAGTGCAGGTCAGTCTTAGAGCACTTGCCCATGGCCGTCCAAGAGCGAGAGCAGAAACTGAG
  TGTGCAGCGGGACCAGATCAGGGAACTGGAGAACGATCGGGAGGCCCAGAGGAGTGTCCTGGAACACCAG
  CTTCTGGACCTGGAGCAGAAGGCTCAAGTGATTGAGTCCCAGAGGGGACAGATTCAGGATCTGAAAAAGC
  AACTGGGGACACTGGAATGCCTGGCCCTGGAACTGGAGGAAAGCCATCACAAAGTGGAGAGCCAACAGAA
  GATGATCACAGAACTAGAGGGCCAGAGGGAAATGCAGAGGGTGGCTCTAACCCACCTCACACTGGACCTG
  GAAGAAAGGAGCCAGGAGCTGCAGGCACAAAGCAGCCAGCTCCATGAGCTGGAGAACCATAGCACCCATC
  TGGCAAAAGAGCTGCAGGAGAGGGACCAGGAGGTGACGTCCCAACGCCAGCAGATCGATGAGCTGCAGAA
  GCAGCAGGAACAGCTGGCTCAGGCCCTGGAGAGGAAAGGCCAGGAGCTGGTGCTGCAGAAGGAAAGGATT
  CAGGTTCTGGAAGATCAGAGGACGCTGCAGACCAAGATCTTAGAGGAGGACCTAGAACAGATCAAGCATT
  CCTTGAGAGAACGCAGCCAGGAGCTGGCCTCTCAGTGGCAGCTGGTGCATGAGCGTGCCGACGATGGAAA
  GAGCCCGAGTAAAGGCCAGCGAGGGAGCCTGGAGCACTTGAAGCTGATCCTGCGTGATAAGGAGAAGGAG
  GTAGAGTGCCAGCAGGAGCGCATCCAGGAACTTCAGGGCCACATGGGCCAGCTGGAACAGCAGCTGCAGG
  GTCTGCACAGGAAGGTGGGGGAGACTAGCCTGCTCCTGACCCATCGAGAGCAGGAGACAGCAACCCTGCA
  GCAGCATCTCCAGGAAGCCAAGGAACAAGGGGAACTGAGAGAGCAGGTACTTCAGGGTCAGCTGGAGGAG
  GCCCAGAGAGATCTGGCCCAGAGGGACCATGAACTAGAGACCCTCCGGCAGGAAAAACAGCAGACCCAGG
  ACCAGGAGGAAAGCATGAAGCTAAAGACAAGTGCTCTGCAGGCCGCTCTGGAGCAGGCCCATGCGACACT
  GAAGGAGCGTCAGGGGGAGCTGGAAGAACACAGGGAACAGGTGCGAAGGCTCCAGGAAGAACTAGAAGTG
  GAGGGACGGCAGGTACGGGCCCTGGAGGAGGTGTTGGGGGACCTAAGGGCTGAGTCTCGGGAGCACGAGA
  AGGCTGTGCTGGCCCTTCAACAACGGTGTGCTGAGCAGGCCCAGGAACATGAGGCAGAAGCCAGGACCCT
  GCAGGACAGCTGGCTGCAGGCCCAGGCCACTCTCACAGAACAAGAACAAGAGCTAGCGGCACTGCGAGCG
  GAAAACCAGTATTCCCGCCGACAGGAAGAGGCTGCAGTTAGCCAGGCAGAGGCGCTGCAGGAGGCCCTCA
  GCAAAGCCCAGGCTGCCCTGCAGGAGAAAGAGCAGAGTCTGCTTGAACAGGCTGAGTTGAGCCACACCCT
  GGAGGCCAGCACTGCCGCCTTGCAAGCTACCCTGGACACCTGCCAGGCAAGTGCCCGGCAGCTGGAGGAG
  GCCCTGAGGATTCGGGAAGGTGAGATCCAGGCCCAGGCTCTCCAACACCACGAGGTCACGCAGCACCTCC
  AGCAGGAACTTTGCCAGAAGAAAGAAGAGCTGAGGCAGCTACTAGAGAAGGCTGGGGCCCGGAGGAGTCA
  AGAAAATGGAATCCAAGAGAAGCAAAGTCTGGAGCAAGAGAGACAAGAGGAAACAAGGAGGCTCCTTGAG
  AGCCTAAAGGAGCTACAGCTGACTGTAGCCCAAAGGGAAGAAGAGATTCTGATGCTGAGGGAGGCCTCGT
  CCCCACGCCACAGAGCCCTCCCAGCCGAGAAGCCAGCTCTGCAACCGCTCCCAGCACAACAGGAGTTAGA
  GCGGCTGCAGACTGCCCTAAGGCAAACAGAGGCCAGGGAGATTGAATGGAGGGAGAAGGCTCAGGACCTG
  GCTCTGTCCCTGGCCCAAAGCAAGGCCAGCATCAGCAGTCTGCAGGAGATAACCATGTTCCTACAAGCCT
  CTGTTCTAGAGCGGGAATCAGAACAACAAAGACTACAGGAGGAGTTGGTACTCAGCAGACAAGCTCTGGA
  AGAGCAGCAGTCAGGTGGCCCACACTCAACCAGCAGAGCAGACCAGGGACCAAAAGTGGGACAGGGAAGC
  CAGTCTGGAGAGGTAGAGACTGAGCCTAGTCCTGGAGTGGAGGAGAAGGAACGGTTGACACAGAGGCTTG
  AACGTCTGCAGCAAGCGGTGGCAGAGCTGGAAGTTGACCGAAGCAAGCTGCAGTGCCACAATGCGCAGCT
  GCGGACCGCCTTGGAGCAGGTGGAACGAGAACGGAGGAAGCTGAAGAGGGATTCCGTGCGTGCATCCCGG
  GCAGGATCACTGGAGGCCAGAGAAACTATGACTTCATCCCCCACACAGCAGGATGGGAGAGGCAGTCAGA
  GGGGTTCCTCGGACAGCGTGCTTGTGGTTGAGCTTCAGAGAGAGGTAGCCCTGCTGCGAGCCCAGCTGGC
  CTTGGAGCGAAAGCAGAGGCAGGACTACATTGCACGGTCCGTGCAGACCAGCCGTGAGCTAGCAGGCCTG
  CATCACAGCCTGTCTCACTCCCTCCTCACCGTGGCCCAGGCCCCTGAGGCCACTGTTCTGGAGGCTGAGA
  CCCGAAAGCTGGATGAGTCTCTGAATCAGAGTCTGACATCCCCAGGGCCATGTTTGCTACATCCCAGTCT
  GGACACTACTCAAAATACCCACAGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001130000
• Insert Size: 7308 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130000.1, NP_001123472.1
• RefSeq Size: 7977 bp
• RefSeq ORF: 7308 bp
• Locus ID: 16328
• Cytogenetics: 2 H1
• Gene Summary: May be involved in ciliogenesis. Probably plays an important role in centrosome cohesion during interphase.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR and includes an additional in-frame segment compared to variant 1, resulting in a longer isoform (3) compared to isoform 1. Sequence Note: Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.