Cnot1 (NM_178078) Mouse Untagged Clone

CAT#: MC225297

Cnot1 (untagged) - Mouse CCR4-NOT transcription complex, subunit 1 (Cnot1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cnot1
• Synonyms: 6030411K04Rik; AA815922; D830048B13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225297 representing NM_178078
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCTTGACTCGCTCTCGCTGGCCTTGTCTCAAATCAGCTACCTGGTGGACAATTTAACCAAGAAAA
  ACTACCGAGCCAGCCAGCAGGAAATACAGCATATTGTGAATCGGCACGGTCCTGAGGCAGACAGGCATTT
  ATTACGCTGCCTATTTTCACATGTGGATTTCAGTGGCGATGGTAAAAGCAGTGGCAAAGATTTCCATCAG
  ACACAGTTTCTGATTCAGGAGTGTGCCTCGCTGATTACAAAGCCAAATTTTATCTCAACATTGTCATATG
  CCATTGATAATCCATTGCACTACCAGAAGAGTTTAAAGCCTGCACCCCACTTATTTGCCCAGCTGAGTAA
  AGTTCTCAAGCTAAGTAAAGTACAGGAGGTCATTTTTGGTCTTGCCCTTTTGAATTCTTCTAGCCCAGAT
  CTCAGGGGTTTTGCTGCCCAGTTTATTAAACAGAAGCTTCCGGATCTTCTGCGTTCTTACATTGACGCAG
  ACGTCAGTGGAAATCAAGAAGGTGGCTTCCAAGATATAGCAATAGAGGTCCTACACCTCCTACTCTCCCA
  TCTCCTCTTTGGGCAGAAGGGAGCCTTTGGAGTTGGACAGGAACAGATAGACGCCTTTCTTAAGACACTA
  CGCAGAGATTTTCCCCAAGAGCGCTGTCCTGTGGTGCTTGCACCACTTTTATACCCTGAAAAACGGGACA
  TTCTAATGGACAGGATTTTGCCTGATTCTGGAGGGGTAGCTAAAACCATGATGGAGAGCTCGCTAGCTGA
  CTTCATGCAGGAAGTGGGCTATGGCTTTTGTGCAAGTATTGAAGAATGTCGCAATATAATCATGCAGTTT
  GGTGTTCGGGAGGTCACAGCTGCCCAGGTTGCCAGGGTTTTGGGAATGATGGCTCGGACTCATTCAGGAT
  TAACAGACGGTATTCCATTACAGAGCATCTCTGCTCCTGGCAGTGGGATCTGGAGCGACGGAAAAGATAA
  AAGCGAGGGGGCACAGGCACACACGTGGAATGTCGAGGTTTTGATTGATGTTCTTAAAGAGCTGAACCCG
  AGTTTGAATTTCAAGGAAGTAACTTATGAACTGGACCATCCTGGATTTCAGATTCGTGACAGCAAAGGAC
  TTCACAATGTGGTTTATGGCATTCAAAGGGGTCTGGGCATGGAAGTGTTTCCAGTAGATTTTATATATAG
  GCCTTGGAAACATGCAGAAGGCCAGCTCTCCTTCATCCAGCATTCCCTGATAAACCCAGAGGTCTTCTGT
  TTTGCTGACTACCCCTGTCACACTGTTGCCACTGATATTTTGAAAGCACCACCAGAGGATGACAATCGAG
  AAATTGCCACATGGAAAAGCTTGGATTTGATTGAATCTCTGCTGAGGCTTGCAGAGGTTGGGCAGTATGA
  GCAAGTGAAACAACTCTTCAGCTTCCCTATCAAACACTGTCCAGACATGTTGGTATTGGCCTTACTTCAA
  ATCAACACTTCCTGGCACACCCTACGCCATGAACTCATCTCTACTTTGATGCCAATTTTCCTTGGAAACC
  ATCCTAACTCTGCCATTATTTTGCACTATGCATGGCATGGGCAGGGGCAGTCTCCATCAATTCGCCAACT
  TATAATGCATGCAATGGCAGAATGGTACATGAGAGGGGAGCAGTATGATCAGGCCAAATTGTCTCGAATA
  CTTGACGTGGCCCAGGACTTGAAGGCCCTGTCGATGCTGCTGAACGGCACTCCATTTGCTTTTGTCATTG
  ACCTTGCTGCACTCGCTTCACGGCGGGAATACCTCAAACTTGACAAGTGGCTAACAGATAAGATAAGAGA
  GCATGGGGAGCCTTTTATACAAGCATGTATGACTTTTTTAAAGAGAAGGTGCCCTTCTATTTTGGGTGGA
  CTTGCCCCAGAGAAAGATCAGCCCAAAAGTGCTCAACTTCCCGCTGAAACTCTGGCAACAATGTTGGCTT
  GTCTGCAGGCTTGTGCAGGGAGTGTTTCTCAAGAGTTATCAGAGACCATCCTCACCATGGTAGCCAATTG
  CAGTAATGTAATGAATAAGGCCAGACAGCCACCACCTGGAGTTATGCCAAAAGGTCGCCCTCCTAGTGCT
  AGCAGCTTAGATGCCATTTCTCCTGTTCAGATTGACCCTCTTGCTGGAATGGCATCTCTTAGTATAGGTG
  GTTCGGCTGCCCCTCACACCCAGAGCATGCAGGGTTTTCCTCCAAATCTGGGTTCTGCGTTCAGTACCCC
  TCAGTCACCAGCAAAAGCATTTCCACCACTTTCAACCCCAAATCAAACAACGGCCTTCAGTGGGATTGGA
  GGACTCTCCTCACAGCTTCCAGTAGGTGGTCTTGGCACAGGAAGCCTGACTGGCATAGGAACTGGGGCCC
  TTGGACTTCCTGCAGTGAACAATGATCCTTTTGTACAGAGGAAACTGGGCACCTCTGGACTCAACCAGCC
  TACATTCCAGCAGACCGACTTGTCTCAGGTGTGGCCAGAGGCAAATCAGCACTTTAGTAAAGAAATTGAT
  GATGAAGCAAACAGTTATTTCCAGCGAATATATAATCACCCACCACATCCAACCATGTCTGTTGATGAGG
  TATTAGAAATGCTACAGAGATTTAAAGACTCTACAATAAAAAGGGAACGAGAAGTATTTAACTGTATGCT
  GAGGAACTTGTTTGAAGAATACCGGTTTTTCCCTCAGTACCCTGATAAAGAATTACATATAACAGCTTGC
  TTGTTTGGTGGCATAATTGAAAAAGGACTGGTCACTTACATGGCATTGGGTCTGGCCCTGCGATATGTTC
  TTGAAGCCTTACGGAAGCCTTTTGGATCCAAAATGTATTATTTTGGTATTGCTGCACTAGATAGATTTAA
  AAATAGATTGAAGGACTATCCTCAGTATTGTCAGCACCTGGCTTCTATCAGCCACTTTATGCAGTTCCCA
  CATCATTTGCAGGAGTACATTGAGTACGGACAGCAGTCTCGAGATCCTCCTGTGAAAATGCAGGGCTCTA
  TTACTACCCCTGGAAGTATTGCACTGGCTCAGGCCCAGGCTCAGGCCCAGGTTCCAGCAAAAGCTCCTCT
  TGCCGGTCAGGTTAACACAATGGTAACCACCTCAACAACCACCACTGTTGCTAAAACGGTTACAGTCACC
  AAGCCAACGGGAGTCAGCTTTAAGAAAGATGTGCCACCTTCTATAAATACAACAAATATCGATACATTGC
  TTGTGGCCACAGATCAAACTGAGAGGATTGTGGAGCCCCCAGAAAATATTCAAGAGAAAATTGCTTTTAT
  TTTCAACAATCTCTCCCAGTCAAATATGACACAGAAGGTTGAAGAGCTTAAAGAAACTGTGAAGGAAGAA
  TTTATGCCTTGGGTTTCCCAGTATCTGGTTATGAAAAGAGTCAGTATTGAGCCAAACTTTCATAGCCTGT
  ATTCAAACTTTCTTGATACATTGAAGAATCCTGAATTTAACAAGATGGTTCTGAATGAAACCTACAGAAA
  CATTAAAGTGCTGCTGACTTCTGATAAAGCTGCGGCCAATTTCTCAGATCGTTCTTTGCTGAAGAACTTG
  GGACATTGGTTAGGAATGATCACATTAGCTAAAAATAAACCCATCTTACACACAGACTTGGATGTGAAAT
  CTTTGCTGTTAGAGGCTTACGTTAAAGGACAGCAAGAGTTGCTCTATGTGGTGCCCTTTGTTGCCAAAGT
  TCTAGAGTCCAGCATTCGCAGCCTGGTTTTTAGACCACCAAACCCCTGGACAATGGCAATTATGAATGTA
  TTGGCTGAGCTGCATCAGGAGCATGACTTAAAGTTAAACCTGAAGTTTGAGATTGAGGTGCTCTGTAAGA
  ACCTTGCATTAGACATCAATGAGCTCAAACCTGGAAACCTGCTGAAAGATAAAGATCGTCTGAAGAACTT
  AGATGAGCAACTCTCTGCTCCAAAGAAAGATGTCAAGCAGCCCGAAGAGCTTCCTGCCATCACAACAACA
  ACAACTTCTACAACACCAGCTACCAGTACTACGTGTACAGCCACAGTTCCACCACAGCCACAGTACAGCT
  ACCACGACATCAATGTCTACTCTCTAGCAGGCCTGGCACCTCATATTACTCTAAACCCTACAATCCCTTT
  ATTTCAAGCTCATCCTCAGCTGAAGCAGTGTGTGCGGCAAGCAATTGAAAGAGCTGTCCAGGAGCTTGTC
  CATCCTGTGGTGGATCGCTCAATTAAGATTGCTATGACTACGTGCGAACAGATAGTCAGAAAGGACTTTG
  CCCTGGATTCTGAGGAGTCTCGAATGAGAATAGCAGCTCATCACATGATGCGGAACTTGACAGCTGGAAT
  GGCTATGATTACTTGCAGGGAACCTTTACTGATGAGCATATCCACCAACCTCAAAAACAGCTTTGCCTCA
  GCCCTGCGTACTGCATCCCCACAGCAAAGGGAAATGATGGACCAGGCTGCTGCTCAGTTAGCTCAGGACA
  ATTGTGAATTAGCTTGTTGTTTTATTCAGAAAACTGCAGTGGAGAAAGCAGGCCCTGAGATGGACAAGAG
  ATTGGCAACTGAATTTGAGCTGAGGAAGCATGCCCGGCAAGAAGGACGTAGGTACTGTGATCCTGTGGTC
  TTAACCTACCAAGCCGAGCGGATGCCGGAGCAGATCAGGCTGAAAGTTGGTGGTGTGGACCCAAAGCAGC
  TAGCCGTCTATGAAGAGTTTGCACGAAATGTGCCTGGCTTCTTACCTACAAATGACTTAAGTCAGCCTAC
  AGGGTTTTTAGCTCAGCCCATGAAGCAAGCTTGGGCAACTGATGATGTAGCGCAGATTTATGATAAGTGT
  ATTACAGAACTGGAGCAGCACCTACACGCCATTCCACCCACTCTGGCCATGAATCCTCAAGCCCAGGCTC
  TCCGAAGTCTTTTAGAGGTTGTAGTGTTGTCTCGGAATTCTCGGGATGCGATAGCTGCTCTTGGATTACT
  ACAGAAGGCTGTAGAAGGCTTATTAGATGCCACAAGTGGTGCTGACGCCGACTTACTGCTGCGATACAGG
  GAGTGCCACCTGTTGGTACTGAAAGCTCTGCAGGATGGCAGGGCATATGGATCTCCATGGTGCAACAAAC
  AAATCACAAGGTGCCTAATTGAATGTCGAGATGAATATAAATATAATGTGGAGGCTGTGGAATTGTTAAT
  TCGAAATCATTTGGTTAACATGCAGCAGTATGATCTTCATCTTGCTCAGTCGATGGAGAATGGCTTGAAC
  TATATGGCCGTGGCGTTTGCTATGCAACTGGTAAAAATTCTGCTGGTGGATGAAAGGAGTGTTGCCCATA
  TTACTGAGGCAGATCTGTTCCATACCATTGAAACCCTCATGAGAATTAATGCTCATTCCAGAGGCAATGC
  TCCAGAAGGGTTGCCCCAGCTGATGGAAGTGGTGAGATCCAACTATGAAGCAATGATTGATCGTGCTCAT
  GGAGGCCCTAACTTTATGATGCATTCTGGGATCTCTCAAGCCTCAGAATATGATGACCCTCCAGGCCTGA
  GGGAGAAGGCAGAGTATCTTCTGAGGGAATGGGTGAATCTCTACCATTCAGCAGCAGCTGGCCGCGACAG
  TACCAAAGCATTCTCTGCGTTTGTTGGACAGATGCACCAACAAGGAATACTTAAAACTGATGACCTCATA
  ACAAGGTTTTTTCGTCTTTGCACTGAAATGTGTGTTGAAATCAGTTACCGTGCTCAGGCGGAGCAGCAGC
  ACAATCCTGCGGCTAACCCTACCATGATCCGGGCCAAGTGCTACCACAACCTGGATGCCTTTGTCCGCCT
  CATTGCACTGTTAGTGAAGCACTCTGGGGAGGCCACCAACACTGTCACAAAGATCAACCTGCTCAATAAG
  GTTCTCGGTATAGTAGTGGGAGTTCTCCTACAGGATCATGATGTTCGGCAGAGTGAATTTCAGCAACTTC
  CGTACCATAGGATTTTTATCATGCTGCTGTTGGAGCTCAATGCACCCGAGCATGTGTTGGAAACCATTAA
  TTTCCAGACTCTTACTGCTTTCTGCAATACATTCCACATCTTGAGACCTACTAAAGCTCCTGGCTTTGTA
  TATGCTTGGCTCGAATTGATTTCCCATCGGATCTTTATTGCAAGAATGCTGGCACATACGCCACAGCAGA
  AGGGGTGGCCTATGTATGCTCAGCTGTTGATTGATTTATTCAAATATTTAGCACCTTTCCTTAGAAATGT
  GGAACTCACTAAACCTATGCAAATCCTCTACAAGGGTACTCTGAGAGTGCTGCTGGTTCTTTTGCATGAT
  TTCCCAGAGTTCCTTTGTGATTACCATTATGGGTTCTGTGATGTGATCCCTCCTAATTGTATCCAGTTAA
  GAAATTTGATCCTGAGTGCCTTCCCAAGAAACATGAGGCTCCCAGACCCATTCACTCCTAATCTGAAGGT
  GGACATGTTAAGCGAAATTAACATCGCACCTCGGATCCTCACCAATTTCACTGGAGTGATGCCGCCTCAG
  TTCAAAAAGGATTTGGATTCTTATCTTAAAACTCGGTCGCCGGTCACTTTCCTGTCTGATCTGCGTAGTA
  ACCTACAGGTATCTAATGAGCCCGGGAATCGCTACAACCTCCAGCTTATCAATGCATTGGTGCTCTACGT
  AGGGACTCAGGCTATCGCACACATCCACAACAAGGGCAGCACGCCCTCAATGAGCACCATCACCCACTCA
  GCACACATGGACATCTTCCAGAACCTGGCTGTGGACCTAGACACTGAGGGCCGATATCTCTTCTTAAATG
  CAATTGCGAACCAGCTACGGTACCCAAACAGCCACACTCACTACTTCAGCTGCACAATGTTGTACCTTTT
  TGCTGAGGCGAACACTGAGGCAATCCAGGAACAGATCACAAGGGTTCTCTTGGAACGGTTGATTGTAAAT
  AGGCCACATCCTTGGGGTCTTCTTATCACCTTCATTGAGCTGATTAAAAACCCAGCATTTAAGTTCTGGA
  ACCATGAATTTGTACACTGTGCCCCAGAGATTGAAAAGTTATTTCAGTCTGTTGCACAGTGCTGCATGGG
  ACAGAAGCAGGCTCAGCAAGTGATGGAGGGGACCGGTGCCAGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178078
• Insert Size: 7116 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178078.2, NP_835179.1
• RefSeq Size: 8375 bp
• RefSeq ORF: 7116 bp
• Locus ID: 234594
• UniProt ID: Q6ZQ08
• Cytogenetics: 8 D1
• Gene Summary: Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3' UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity; prevents their differentiation towards extraembryonic trophectoderm lineages.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has an additional in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 2, which is longer than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.