Plce1 (NM_019588) Mouse Untagged Clone

CAT#: MC225255

Plce1 (untagged) - Mouse phospholipase C, epsilon 1 (Plce1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Plce1
• Synonyms: 4933403A21Rik; mKIAA1516; Plce; PLCepsilon
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225255 representing NM_019588
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGTCGGAAGAAATGGCCGCTTCTGTCCTCATCCCTGTGACTCAAAGGAAAGTGGCTTCTGCCCAGT
  CGGTGGCAGAAGAGAGGAGTGTAAAGGTCTCAGATGCGGGGATTCCTCGGGCACGTGCTGGCAGGCAGGG
  TGCGCTCATCCCTCCTACCATCTCACAGTGGAACAAACATAAGGAAGAGTCGTCTAGAAGTGATTTGTCC
  AAGGTCTTCTCAATAGCGAGGGGGGAACTAGTCTGCGATGAGAATTCCAACGAAGAGGGCTGGGAGGAAA
  ACGCACCGGACTCCCCGGAAAACCACGCGATGAACGGCAACAGTTTAGTGCAAAGCCACCAGCACCAGTT
  TCCCCGGAGCCAGCTTTGTGAAGCCCGTGACTCTGTCACAGAAGACCCGTGTTTGCAGCCTGGAATTCCT
  TCTCCCCTGGAAAGGAAGGTGCTCCCTGGAATTCAACTGGAGATGGAGGACTCTCCCATGGACGTGAGCC
  CTGCGGGAAGTCAGCCTAGGATCATGGAGTCCAGCGGACCTCACTCTGACCGCAACACGGCGGTATTTCA
  CTTCCATTATGAAGCTGACAGGACAATGTCGGATGCTTTTCATACCCTGTCAGAAAACCTGATTTTGGAT
  GACTGTGCAAATTGTGTTACTCTTCCTGGGGGGCAGCAAAATAAAAATTGCATGGCGTATGCTTGCAAAC
  TGGTGGAGTTGACAAGAACCTGTGGAAGTAAGAATGGGCAAGTCCAGTGTGAGCACTGTACCTCGCTGCG
  CGATGAATACCTGTGCTTCGAAAGCTCCTGCTCAAAGGCCGATGAGGTCTGCTCAGGTGGTGGCTTTTGC
  GAGGACGGCTTTGCTCACGGTCCTGCTGCCAAGACTTTTCTGAACCCTCTGGAGGATTTCTCTGACAATT
  GTGAAGACGTAGACGATTTTTTTAAAAGCAAAAAGGAGCGGTCCACGTTGTTAGTCCGAAGGTTTTGTAA
  AAATGACAGGGAAGTTAAGAAGTCTGTGTATACTGGGACCAGAGCCATCATGAGAACTCTGCCTTCTGGC
  TGTATTGGGCCAGCTGCTTGGAATTACGTTGATCAGAAGAAAGCTGGTCTCCTATGGCCTTGTGGGAATG
  TAATGGGAACTCTGTCAGCAATGGACATAAGGCAAAGTGGGAGCCAACGTCTGTCTGAAGCCCAGTGGTG
  CCTGATCTACAGTGCCGTGAGAAGAGGGGAAGAAATAGAAGATACGGTTGGATCTCTTCTCCATTGCTCC
  ACACAGCTCCCAAACTCTGAGACAGCCCATGGAAGGATTGAAGATGGTCCCTGCTTAAAGCAGTGTGTAC
  GAGACACTGAATGTGAATTCCGTGCTACCCTCCAAAGGACATCCATAGCTCAGTACATCACCGGTTCTCT
  TCTAGAGGCAACTACATCTTTAGGAGCAAGAAGTGGCCTTCTCAGTTCCTTCGGAGGATCCACTGGACGA
  ATAATGCTGAAAGAACGCCAACTGGGCACCTCTATGGCCAATTCCAATCCTGTTCCTTCAAGTTCAGCTG
  GAATCAGCAAGGAGCTGATTGATCTCCAGCCCCTCATTCAGTTCCCGGAGGAAGTAGCCAGCATCTTAAC
  AGAGCAGGAACAGAACATTTACCGCAGGGTCCTGCCCATGGACTACCTCTGCTTCTTAACCCGGGACTTG
  AGTTCACCTGAATGCCAGAGATCACTGCCCCGTCTCAAAGCATCCATCTCAGAATCCATCCTCACGTCTC
  AAAGTGGAGAACACAATGCCCTTGAAGACCTTGTGATGAGATTTAACGAGGTGAGCTCCTGGGTAACATG
  GCTGATCCTCACAGCAGGCTCCATGGAGGAGAAGCGGGAAGTCTTTTCTTATTTGGTGCATGTGGCCAAA
  TGCTGCTGGAACATGGGCAACTACAACGCTGTCATGGAGTTCCTGGCTGGCCTCAGGTCAAGGAAAGTTC
  TAAAGATGTGGCAGTTCATGGACCAGTCTGACATTGAGACCATGCGGAGCCTGAAGGATGCCATGGCCCA
  GCATGAGTCTTCCGTGGAGTACAAGAAGGTAGTGACCCGAGCCTTGCACATCCCCGGCTGCAAAGTGGTC
  CCCTTCTGTGGGGTGTTCCTGAAGGAGCTCTGTGAGGTGCTTGATGGTGCCTCTGGCCTCCTGAAGCTCT
  GCCCACGGTACAGCTCCCAAGAAGAAGCTCTGGAGTTTGTAGCCGATTACAGTGGACAAGATAACTTCTT
  ACAACGAGTGGGACAGAATGGCTTAAAGAATTCAGAGAAGGAGCTCACGGTCAACAGCATCTTTCAGGTC
  ATCCGGAGCTGCAGCCGAAGTTTGGAGATGGAGGAGGAAGACAGTGCCAGTGAAGGGAGCGGCTCTAGGA
  AAAACTCCCTGAAGGACAAAGCCCGATGGCAGTTTATAATTGGAGATTTGCTGGATTCAGAAAATGACAT
  CTTTGAGAAGTCCAAAGAGTGTGACCCCCATGGATCCGAGGAGTCACAGAAAGCCTTTGACCATGGGACA
  GAGCTTATCCCATGGTACGTGCTGTCCATCCAAGCAGATGTGCACCAGTTCCTATTGCAGGGGGCCACAG
  TCATCCACTATGACCAGGACACACATCTCTCGGCCCGCTGCTTTCTCCAGTTGCAGCCTGACAATAGCAC
  GTTGACCTGGATGAAACCTCCCACTGCCTCCCCAGCAGGCGCCAGACCCAAGCTCGGTGTGCTTAGTAAC
  ATGGCCGAGCCTGGCAAGTTCCCATCCCCAGGCAACGCCGGGGTGAGCGGCCTGGCAGAGGGAATTCTGG
  ATCTGTTCTCAGTGAAGGCTGTGTACATGGGACACCCTGGCATTGATATACACACTGTGTGTGTCCAGAA
  CAAACTTAGTAGCATGCTTCTATCGGAGACGGGGGTCACACTGCTCTATGGGCTTCAGACCACGGACAAT
  AGATTACTGCACTTTGTGGCGCCAAAGCACACGGCTGAGATGCTTTTCAGTGGATTGCTGGAACTCACTA
  CAGCTGTGAGAAAGATAAGGAGGTTCCCTGACCAACGACAGCAGTGGCTTCGGAAACAGTATGTCAGCCT
  CTATCAGGAGGATGGTCGGTATGAAGGCCCAACCTTGGCACATGCTGTGGAGTTGTTTGGTGGTAGGCGG
  TGGAGCACTAGAAACCCCAGCCCTGGAATGTCAGCAAAGAATGCTGAGAAGCCCAACATGCAGAGAAACA
  ACACCCTGGGCATAAGCACCACCAAGAAAAAGAAGAAAATGCTCATGAGGGGAGAGAGTGGAGAGGTGAC
  GGACGATGAGATGGCTACCCGCAAGGCCAAAATGTACAGAGAGTGTCGCAGCCGGAGCGGCTCTGATCCT
  CAAGACGTTAACGAACAAGAAGAGTCGGAGGCCAATGTCATCACTAACCCTCCCAACCCCCTCCATTCCA
  GAAGAGCCTACTCTTTGACCACGGCTGGGTCCCCCAACTTGGCTACCGGGATGTCATCTCCCATCAGTGC
  CTGGAGCAGCAGCAGCTGGCATGGGCGAATCCGAGGAGGAATGCAAGGCTTCCAGAGCTTCATGGTCTCA
  GACAGTAACATGAGTTTCGTTGAATTCGTCGAGCTGTTCAAATCATTCAGCATCAGGAGCCGCAAGGACT
  TGAAGGACATCTTTGACATCTACTCTGTACCCTGCAATCGGTCTGCCTCGGAGTCAGCCCCACTCTACAC
  CAACCTGACAATCGAAGAAAATACCAGCGATCTTCAGCCTGACCTAGATTTGCTGACTAGAAACGTCTCC
  GACCTGGGGCTGTTCATTAAAAGTAAACAGCAGCTTTCTGACAACCAGAGGCAGATATCTGATGCTATCG
  CTGCTGCAAGCATCGTGACCAATGGTACTGGGATTGAAAGCACGTCCCTGGGCATATTTGGGGTCGGCAT
  ACTCCAGCTCAATGACTTCCTGGTGAATTGCCAAGGAGAACACTGCACATATGATGAAATCCTGAGCATC
  ATCCAGAAATTTGAGCCTAGCGTCAGCATGTGTCATCAAGGCCTACTGTCATTTGAAGGGTTTGCAAGGT
  TTCTGATGGATAAAGACAATTTCGCCTCAAAAAATGATGAGTCACGGGAGAACAAGAAAGAACTGCAGCT
  ACCCCTCTCCTACTATTATATAGAATCTTCACACAACACCTACCTCACCGGCCATCAGCTCAAGGGGGAG
  TCCTCTGTGGAGCTCTATAGCCAGGTCCTCCTGCAAGGATGTAGAAGCATAGAGCTGGACTGCTGGGACG
  GGGATGATGGGATGCCCATCATCTATCATGGGCATACACTGACAACCAAGATCCCCTTCAAGGAAGTGGT
  GGAAGCCATCGATCGCAGTGCCTTCATCACCTCAGACCTGCCAATCATCATATCCATTGAGAACCATTGT
  TCATTGCCTCAACAACGAAAAATGGCAGAAATTTTCAAGAGTGTGTTTGGAGAAAAGCTGGTGGCTAAGT
  TCTTATTCGAGACCGATTTCTCAGATGACCCAATGCTTCCCTCACCTGATCAACTTAGAAGGAAAGTACT
  CCTTAAAAACAAGAAGCTAAAGGCCCATCAGACACCTGTCGACATCCTAAAACAAAAGGCTCATCAGTTA
  GCATCCATGCAAGCACAGGCTTTCACCGGTGGGAATGCCAACCCGCCACCTGCCAGTAATGAGGAAGAGG
  AAGATGAGGAGGATGAATATGATTACGATTACGAGTCCCTGTCCGATGACAATATTCTGGAAGACAGACC
  TGAGAACAAATCATGCGCTGACAAGCTTCAGTTTGAGTACAATGAAGAGGTCCCCAAGAGGATAAAGAAA
  GCAGACAACTCCTCTGGCAATAAGGGAAAGGTGTATGACATGGAACTGGGAGAGGAGTTTTACCTTCCTC
  AGAACAAAAAGGAAAGCAGGCAGATTGCACCGGAGCTTTCTGACCTCGTCATTTATTGCCAGGCAGTAAA
  ATTTCCAGGCCTGTCAACTCTAAATTCATCTGGCTCTAGCAGAGGGAAAGAAAGGAAAAGCAGGAAGTCC
  ATTTTTGGCAACAATCCTGGCAGAATGAGCCCCGGGGAGACAGCGCCATTTAACAGAACATCTGGAAAAG
  GTTCCTGTGAAGGGATGCGACACACCTGGGAGGAATCTTCTCCACTCAGCCCAAGCACATCCCTCAGCGC
  TATCATCAGGACTCCTAAATGCTATCACATCTCCTCACTGAACGAGAACGCCGCCAAACGTCTGTGCCGC
  AGGGGTTCTCAGAAACTGATCCAGCACACCGCTTATCAGCTGCTGAGGACCTACCCGGCAGCGACCAGAA
  TCGACTCCTCCAATCCCAACCCCATCATGTTCTGGCTCCATGGGATACAGCTTGTAGCTCTAAACTACCA
  AACCGATGACCTTCCTTTACATTTAAACGCCGCCATGTTTGAGGCGAATGGTGGGTGTGGTTATGTTTTG
  AAGCCCCCTGTCCTGTGGGATAAAAGCTGTCCCATGTACCAGAAGTTTTCTCCACTGGAAAGAGACCTAG
  ACAACCTGGATCCTGCTATCTATTCTTTAACTATCATCTCTGGCCAGAATGTGTGCCCTAGTAACAGCAC
  CGGAAGTCCATGCATTGAAGTCGACGTCTTGGGCATGCCCCTGGACAGCTGCCATTTCCGCACAAAACCC
  ATCCATCGAAACACTCTAAACCCCATGTGGAATGAGCAGTTTCTCTTCCGGGTTCACTTTGAAGATCTTG
  TATTTCTTCGTTTTGCAGTCGTGGAAAACAACAGTTCGGCGATAACGGCTCAGAGAATCATTCCACTCAG
  AGCTTTGAAACGAGGATATCGACATCTTCAGCTGCGGAACCTCCACAATGAAATCTTGGAAATCTCTAGC
  TTATTCATAAACAGCAGAAGGATGGAAGAAAACCCCTCTGGCAGTTCCATGCCAGCCTCGTTGATGTTTA
  ATACTGAAGAGAGGAAATGTTCTCAGACTCACAAAGTCACAGTGCATGGTGTCCCAGGTCCAGAGCCCTT
  TGCTGTTTTCACTATAAACGAAGGCACCAAGGCAAAGCAGCTTCTCCAACAGGTTTTGGCAGTTGACCAA
  GACACCAAATGTACTGCCACAGACTATTTTTTGATGGAAGAGAAGCATTTTATATCTAAGGAAAAGAATG
  AATGCAGGAAACAACCATTCCAGAGAGCTGTGGGTCCAGAAGAAGATATTGTGCAGATTTTAAACAGTTG
  GTTCCCAGAAGAAGGCTACGTAGGCAGGATTGTCTTAAAGCCCCAACAGGAAACTCTAGAAGAGAAAAGC
  ATTGTCTTCGACGACAAAGAGGTGATCTTGAGCTCAGAGGAGGAGAGTTTCTTTGTGCAAGTGCATGATG
  TCTCTCCAGAGCAACCTCGAACTGTCATCAAAGCCCCACGTGTCAGCACTGCACAGGATGTAATTCAGCA
  GACCTTATGCAAAGCCAAATATTCCTACAGCATCCTGAACAACCCCAACCCATGTGACTATGTGCTTTTG
  GAAGAGGTGCTGAAAGATGCAGCTAATAAGAAGTCTTCTACCCCAAAGTCCTCGCAGAGGATCCTCTTGG
  ACCAAGAATGTGTGTTTCAAGCGCAAAGCAAGTGGAAGGGTGCGGGAAAGTTCATACTTAAGCTAAAGGA
  GCAGGTGCAGGCATCCCGAGAAGACAAAAGGAGGGGTATCTCCTTTGCAAGTGAACTCAAGAAGCTCACT
  AAGTCCACTAAGCAGTCTCGAGGGCTCCCATCACCTCCTCAGCTTGTGGCCTCAGAAAGTGTTCAAAGCA
  AGGAGGAGAAACCTGTGGGTGCCTTGTCCTCTAGTGACACAGTGGGTTACCAGCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019588
• Insert Size: 6849 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019588.2, NP_062534.2
• RefSeq Size: 9525 bp
• RefSeq ORF: 6849 bp
• Locus ID: 74055
• UniProt ID: Q8K4S1
• Cytogenetics: 19 C3
• Gene Summary: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine-exchange factor (RasGEF) activity. As an effector of heterotrimeric and small G-protein, it may play a role in cell survival, cell growth, actin organization and T-cell activation.[UniProtKB/Swiss-Prot Function]