Cacna1e (NM_009782) Mouse Untagged Clone

CAT#: MC225252

Cacna1e (untagged) - Mouse calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cacna1e
• Synonyms: A430040I15; alp; alpha1E; BII; Cach6; Cacnl1a6; Cav2; Cav2.3; Cchr; Cchra1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225252 representing NM_009782
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCGCTTCGGGGAGGCGGTGGTCGTTGGCAGGCCAGGCTCAGGCGATGGAGACTCGGACCAGAGCA
  GGAACCGACAAGGAACCCCCGTCCCGGCCTCGGGGCCGGCGGCCGCCTACAAGCAGTCAAAAGCGCAGAG
  GGCGCGGACTATGGCTTTGTACAACCCCATACCAGTCCGGCAGAACTGTTTCACGGTCAACAGATCCCTG
  TTCATCTTCGGAGAAGATAACATTGTCAGGAAATACGCCAAGAAGCTCATCGATTGGCCGCCATTTGAGT
  ACATGATCCTGGCCACCATCATTGCCAACTGCATCGTCTTGGCCCTGGAGCAGCATCTTCCTGAGGATGA
  CAAGACCCCAATGTCTCGAAGACTGGAGAAGACAGAACCATATTTCATTGGGATCTTCTGCTTTGAAGCT
  GGGATCAAAATTGTGGCTCTAGGGTTCATCTTCCATAAGGGTTCATACCTCCGTAATGGCTGGAATGTCA
  TGGACTTCATCGTGGTCCTTAGTGGCATCCTGGCCACTGCGGGAACCCACTTCAACACCCACGTGGACCT
  GCGGACCCTCCGGGCTGTGCGTGTCCTGAGGCCTTTAAAGCTGGTATCAGGAATACCTAGCCTGCAGATC
  GTGCTGAAGTCTATCATGAAGGCCATGGTGCCTCTTCTGCAGATTGGCCTTCTGCTCTTCTTTGCCATCC
  TCATGTTTGCTATCATTGGCTTGGAGTTCTACAGTGGCAAGTTGCATCGAGCATGCTTCATGAACAATTC
  AGGTATTCTAGAGGGGTTTGATCCTCCTCACCCGTGTGGTGTGCAAGGCTGCCCGGCTGGTTATGAATGT
  AAGGACTGGATCGGCCCCAATGACGGGATCACCCAGTTTGACAACATCCTTTTTGCCGTGCTGACTGTCT
  TCCAATGCATCACTATGGAAGGGTGGACCACTGTGCTGTACAATACCAATGATGCCTTAGGAGCCACCTG
  GAACTGGCTGTACTTCATCCCTCTCATCATCATCGGATCCTTCTTTGTTCTCAACCTCGTCCTGGGAGTG
  CTTTCCGGGGAATTTGCCAAAGAAAGAGAGAGAGTGGAGAACCGAAGAGCGTTCATGAAGCTCCGGCGCC
  AGCAACAGATTGAGCGAGAATTGAACGGCTACCGTGCCTGGATAGACAAAGCAGAGGAAGTCATGCTTGC
  TGAAGAAAATAAAAACTCGGGAACATCAGCCTTGGAAGTGCTTCGAAGGGCAACCATCAAAAGGAGCCGG
  ACGGAGGCCATGACCCGAGACTCCAGCGATGAGCACTGCGTTGATATCTCTTCAGTGGGCACACCTCTTG
  CCAGAGCCAGTATCAAGAGCACAAAAGTAGATGGGGCCTCCTATTTCCGGCACAAGGAACGGCTTCTGCG
  CATCTCTATCCGCCACATGGTCAAGTCCCAAGTCTTTTACTGGATCGTCCTGAGTGTTGTGGCTCTTAAT
  ACTGCCTGTGTGGCCATTGTTCATCACAACCAGCCACAGTGGCTCACTCACCTCCTCTACTATGCAGAGT
  TTTTGTTTCTGGGACTCTTCCTGTTGGAGATGTCCCTGAAGATGTATGGCATGGGGCCACGCCTCTATTT
  TCACTCATCCTTCAACTGCTTTGATTTTGGGGTCACAGTGGGCAGCATCTTTGAGGTTGTCTGGGCAATC
  TTCAGACCTGGCACATCTTTTGGAATCAGTGTCTTACGAGCTCTCCGGCTCCTAAGGATATTTAAAATAA
  CCAAGTATTGGGCATCCCTCCGGAATCTGGTTGTCTCCTTGATGAGCTCCATGAAGTCTATCATCAGCTT
  ACTCTTCCTTCTCTTCCTCTTCATCGTTGTCTTTGCTCTCCTGGGAATGCAGCTCTTTGGAGGCAGGTTT
  AACTTTAATGATGGGACTCCTTCAGCTAATTTTGACACCTTCCCTGCAGCTATCATGACAGTGTTCCAGA
  TCCTGACAGGGGAAGACTGGAATGAGGTGATGTACAATGGCATTCGCTCCCAGGGTGGGGTCAGCTCAGG
  CATGTGGTCTGCCATCTACTTCATTGTGCTCACCTTGTTTGGAAACTACACGCTGCTAAATGTATTCTTG
  GCCATCGCTGTGGACAATCTGGCCAATGCCCAGGAACTGACCAAGGATGAGCAGGAGGAAGAAGAAGCCT
  TCAACCAGAAACATGCACTGCAGAAGGCCAAGGAGGTCAGCCCGATGTCTGCTCCCAACATGCCTTCCAT
  TGAAAGAGACAGAAGGAGAAGACACCACATGTCGATGTGGGAGCCACGCAGCAGCCACCTGAGGGAGCGG
  AGGCGCCGGCACCACATGTCTGTGTGGGAACAACGCACCAGCCAGCTGAGGAGGCACATGCAAATGTCTA
  GCCAAGAAGCCCTCAACAAAGAGGAGGCCCCGCCAATGAACCCCCTCAATCCACTCAACCCACTGAGTCC
  TCTCAACCCCCTCAATGCTCACCCCAGCCTTTATCGGAGGCCCAGGCCCATTGAGGGCCTAGCCCTAGGT
  CTGGGTCTTGAAAAGTGTGAAGAAGAACGCATCAGTCGTGGGGGCTCCCTCAAGGGGGACATAGGGGGCC
  TGACTAGCGCCCTGGACAACCAGAGGAGCCCCTTGTCCTTGGGCAAACGGGAGCCACCGTGGCTGCCTAG
  ATCCTGTCATGGAAACTGTGACCCAATCCAGCAGGAGGCTGGGGGAGGAGAGACTGTGGTGACTTTTGAG
  GACCGGGCCAGGCACAGGCAGAGTCAGAGGCGAAGCCGGCATCGCCGAGTCAGGACTGAGGGCAAGGACT
  CTGCCTCTGCATCCCGAAGCAGGTCTGCCAGCCAGGAGCGGAGTCTGGATGAAGGGGTGTCCGTTGAGGG
  GGAGAAGGAACATGAGCCTCACAGCAGCCACAGGAGCAAAGAGCCGACCATCCACGAAGAGGAGAGAACC
  CAGGACTTAAGGAGGACCAACAGTCTGATGGTCCCCAGGGGCTCTGGACTGGTAGGGGCTCTTGATGAAG
  CAGAAACACCTCTAGTCCAGCCCCAACCTGAGCTGGAAGTGGGGAAGGATGCAGCTCTGACAGAGCAGGA
  GGCTGAAGGCAGCAGTGAACAAGCCCTTCTTGGGGACGTACAGCTGGATGTGGGCCGGGGCATTAGCCAG
  AGTGAACCGGATCTCTCCTGCATGACAGCCAACATGGACAAGGCTACCACGGAGAGCACCAGTGTCACGG
  TCGCCATCCCTGATGTGGACCCCTTGGTGGACTCGACAGTGGTGAACATTAGCAACAAGACGGATGGAGA
  AGCCAGTCCCTTGAAGGAGGCAGAGACTAAAGAGGAAGAAGAAGAGGTAGAGAAGAAGAAGAAGCAGAAG
  AAGGAGAAGCGAGAGACTGGAAAAGCCATGGTGCCCCATAGCTCTATGTTCATCTTCAGCACCACTAACC
  CGATCCGCAGGGCCTGCCACTACATTGTGAACCTACGCTACTTTGAAATGTGCATTCTCTTGGTGATTGC
  GGCCAGCAGCATTGCCCTGGCAGCCGAGGACCCTGTACTAACTAACTCGGAGCGTAACAAAGTCCTGAGA
  TATTTCGACTATGTTTTCACAGGAGTGTTTACCTTTGAGATGGTTATAAAGATGATAGACCAAGGCTTGA
  TCCTGCAGGATGGATCATACTTCAGAGACCTCTGGAACATCCTGGACTTTGTAGTAGTGGTTGGTGCATT
  GGTAGCATTTGCTCTGGCGAACGCTTTGGGAACCAACAAGGGGAGGGATATCAAGACTATCAAGTCTCTG
  AGGGTACTTCGAGTTCTGCGGCCACTGAAAACCATCAAGCGCTTGCCCAAGCTGAAGGCTGTGTTTGACT
  GCGTGGTGACCTCCTTGAAGAATGTCTTCAACATACTCATTGTCTACAAGCTCTTCATGTTCATCTTTGC
  CGTCATCGCTGTTCAGCTATTCAAAGGAAAGTTCTTTTATTGCACTGACAGTTCCAAGGACACAGAGAAG
  GAGTGCATAGGCAACTATGTAGACCACGAGAAAAACAAGATGGAGGTGAAGGGCCGGGAATGGAAGCGTC
  ATGAATTCCACTATGACAACATCATCTGGGCCCTGCTTACCCTCTTCACAGTCTCTACAGGGGAAGGATG
  GCCTCAGGTTCTGCAGCACTCTGTAGATGTGACAGAGGAGGACAGAGGCCCAAGTCGCAGCAACCGCATG
  GAGATGTCCATCTTTTATGTGGTCTACTTTGTGGTCTTCCCTTTCTTCTTTGTCAATATCTTCGTGGCTC
  TCATTATCATCACCTTCCAGGAGCAAGGGGACAAGATGATGGAGGAGTGTAGCCTGGAGAAGAATGAGCG
  GGCATGCATCGACTTCGCCATTAGTGCCAAACCTCTCACCCGCTACATGCCACAGAACAGGCACACCTTC
  CAGTACCGTGTGTGGCACTTTGTAGTCTCCCCATCCTTTGAGTATACCATCATGGCCATGATTGCCTTGA
  ACACTGTCGTGCTGATGATGAAGTACTACTCTGCTCCTTGCACCTATGAGCTCGCCTTAAAGTACCTGAA
  CATCGCCTTCACTATGGTGTTTTCCCTGGAGTGTGTCCTAAAGGTCATTGCTTTCGGCTTCCTGAACTAT
  TTCCGAGATACCTGGAACATCTTTGACTTCATCACGGTGATCGGCAGTATCACAGAAATTATCCTGACAG
  ATAGCAAGCTGGTGAACACCAGTGGCTTCAATATGAGCTTTCTGAAGCTCTTCCGAGCTGCACGGCTCAT
  AAAGCTTCTGAGACAGGGCTACACCATCCGCATCTTACTGTGGACCTTCGTGCAGTCCTTTAAGGCTCTT
  CCCTACGTCTGCCTTCTCATTGCCATGCTTTTCTTCATCTACGCCATCATTGGGATGCAGGTGTTTGGAA
  ACATAAAGTTAGATGAGGAGAGTCACATCAACCGGCACAACAACTTCCGGAGTTTCTTTGGATCCCTCAT
  GCTGCTTTTCAGGAGTGCCACGGGGGAGGCTTGGCAGGAGATTATGCTGTCCTGTCTCGGTGAGAAGGGT
  TGTGAGCCTGACACCACTGCACCCTCAGGGCAGAATGAGAGTGAGCGCTGTGGCACTGACCTGGCCTACG
  TTTATTTTGTCTCCTTCATCTTCTTCTGCTCCTTCCTGATGCTCAACCTGTTCGTGGCTGTCATCATGGA
  CAACTTTGAGTACCTGACTCGAGATTCCTCCATCCTGGGGCCTCACCACTTAGATGAGTTTGTCCGTGTC
  TGGGCAGAATATGACAGAGCAGCATGTGGCCGCATCCATTACACTGAGATGTATGAAATGCTGACTCTCA
  TGTCACCACCGCTAGGCCTCGGCAAGAGATGTCCCTCCAAAGTGGCCTATAAGAGGCTGGTCCTGATGAA
  CATGCCAGTGGCTGAGGACATGACAGTCCACTTCACCTCCACACTTATGGCTCTGATCCGGACAGCTCTG
  GACATTAAAATCGCCAAAGGTGGTGCAGACAGACAGCAGCTAGACTCGGAGCTGCAAAAAGAGACCCTGG
  CCATTTGGCCTCACCTGTCCCAAAAGATGTTGGATCTGCTTGTGCCCATGCCCAAAGCCTCTGACCTGAC
  TGTGGGCAAGATCTATGCAGCAATGATGATCATGGACTACTATAAACAGAGTAAGGTAAAGAAACAGAGA
  CAGCAGCTGGAAGAACAGAAAAATGCACCCATGTTCCAGCGCATGGAGCCCTCATCACTGCCTCAGGAGA
  TCATTGCTAATGCCAAAGCCCTGCCTTATCTCCAGCAGGACCCTGTTTCTGGCCTGAGTGGTCGGAGTGG
  ATACCCTTCAATGAGTCCACTCTCCCCTCAAGAAATATTCCAGTTGGCTTGTATGGACCCAGCGGATGAT
  GGACAATTCCAGGAACAACAATCTCTGGTGGTAACAGACCCTAGCTCTATGCGACGTTCATTTTCTACAA
  TTCGGGATAAGCGTTCAAATTCCTCCTGGTTGGAGGAGTTCTCCATGGAACGCAGCAGTGAAAACACTTA
  CAAGTCGCGGCGCAGAAGTTACCATTCCTCCCTGAGGCTGTCAGCACACCGCCTGAATTCTGACTCTGGT
  CACAAGTCTGACACTCACCGATCAGGAGGCAGGGAGAGAGGCCGATCCAAAGAACGAAAGCACCTCCTCT
  CTCCAGATGTCTCCCGCTGCAATTCTGAAGAGCGAGGGACCCAGGCCGACTGGGAGTCCCCAGAACGTCG
  CCAGTCCAGGTCACCCAGTGAAGGAAGGTCACAGACCCCCAATAGACAGGGTACCGGTTCCCTAAGTGAG
  AGCTCCATCCCCTCTATTTCCGACACCAGCACCCCAAGACGAAGCCGTCGCCAGCTCCCACCTGTGCCAC
  CAAAGCCTCGTCCCCTCCTCTCCTACAGCTCCCTGATGAGACACACTGGCGGCATCTCTCCACCTCCTGA
  TGGAAGTGAGGGTGGATCCCCACTGGCCTCTCAAGCTCTGGAGAGCAACAGCGCTTGCCTGACCGAGTCT
  TCCAACTCCTTGCACCCCCAGCAGGGCCAGCATCCTTCCCCACAGCACTACATCTCCGAGCCCTATCTGG
  CCCTCCATGAAGACTCCCACGCCTCAGACTGTGGCGAGGAGGAGACACTCACCTTTGAGGCAGCCGTAGC
  CACTAGCTTGGGCCGGTCCAATACCATTGGCTCTGCGCCACCCCTACGGCACAGCTGGCAGATGCCTAAT
  GGGCACTATCGGCGGCGGAGGCGTGGGGGGCCTGGGCCTGGCATGATGTGTGGAGCTGTCAGTGACCTCC
  TGAGTGACACGGAAGAAGACGATAAGTGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009782
• Insert Size: 6822 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009782.3, NP_033912.2
• RefSeq Size: 12697 bp
• RefSeq ORF: 6822 bp
• Locus ID: 12290
• Cytogenetics: 1 66.14 cM
• Gene Summary: This gene encodes an integral membrane protein that belongs to the calcium channel alpha-1 subunits family. Voltage-sensitive calcium channels mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes. Voltage-dependent calcium channels are multi-subunit complexes, comprised of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The isoform alpha-1E gives rise to R-type calcium currents and belongs to the high-voltage activated group. Calcium channels containing the alpha-1E subunit may be involved in the modulation of neuronal firing patterns, an important component of information processing. [provided by RefSeq, Jul 2008]