Rttn (NM_175542) Mouse Untagged Clone

CAT#: MC225233

Rttn (untagged) - Mouse rotatin (Rttn), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rttn
• Synonyms: 4921538A15Rik; AI666264; C530033I08Rik; D230040K24
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225233 representing NM_175542
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCTGGCGGGGCTCATCAGGAAGCTCGGCCATCAGCTGGCGGAGATCAGGGAACGCGCCCTCAAAA
  GTATCCTGTGCAAGATTGAGCACAACTTGATCTGCTATGCTGACTTGATTCAGGAAAGGTTGCTTTTTCT
  CCATTTGCTCGAATGGTTCAATTTCCCATCTGTCCCAATGAAAGAGGAGGTTCTGAACTTGTTAAGCAGA
  TTGGTTAAGTATTCTCCAGCCGTCCAACACTTGATTGACCTTGGTGCAGTAGAGTTTTTATCTAAGCTCC
  GTCCAAATGTGGAGCCAGTAATACAGGCTGAGATTGATGGCATTCTCGATGGACTTTTCATTCTTCCTTC
  AGAAGTTCCTGCACTGTGTTCTTCCAGTTACCACACTGATCAAATTGAATTGTCACAACAACCTGAAGTC
  TTAACAGGATATTTTCCCCAAGACAAAAGTAATTTCCAGCAGATGGAAGTACCACCAGGGCCAGCGGGAA
  ACCAGGCTGTGAAGTGGCTGAGGTTCTCTGTGTTTCCTTGGCTACCCCTGACCACAACAGACAGACATGT
  TCTCTCTTCCAATGAAAGCTCTTTGAGAAGTAGTAACCACACTCTGATCTGGAACACCTGTGAGCTTCTG
  AAGGACGTGATCATGCAAGACTTTCCTGCTGAGATTTTTCTTCAGAGGCCAAAGATTGTTCAGGGCCTTT
  TGTGTCTGGTGAAGCTGGCCTTCGGAGGAGATGGGAAGCATCGTCTAGCATTACAGTCAGTGTCCTGTTT
  GCAGCAGCTGTGCACAAGCTTAAGAAACAGACTCAACTTTTACAGAGACCCCAACTTTTTCTCCAGTAGA
  CAAGACACAGTCTCCCAGAATTCTTCTTTGTCTTACTGTCATGAAGCAAGAGGTCCTTACCATTCCCCAA
  ACCCATCCCCGGGAAGCAGTAGCTCTCGGCCTTCTGTGGTTGGGCGCACAGGCCAGCGACCCCGAGGAGA
  TGGTCAGGACTGGGATGCAGTGTCTTCCAGTGGAAGTAGTAGTCACACGCATGTGAACTCTAGGATATCT
  GTGCATTCTCCTTTGGACGTGGCACCCGTGGATCTGCCGGAGCTGGAAGCAGAGGACACACTGGAGCTGC
  AGTTCCAACAGCTTAGTCTTCCTCAGTTCTGCATCTCCACCCTGGAGGCAGCAGCTCCTCTGCTGAGAAC
  AGGCAGCAGAGAAATGATAATAAGAGTTCTGGAACTGCTTGCAGAGGACATGCTGCTCATTGGACAAGCA
  ATTTCATCAGAAATCTGGGATGATAACAGCCTTTTTGCTATCAATGTGAAAGAGAAACTGCTCCAAGTGT
  TGGGGTCCCTTGGAGAAACCATGTGCTACCGTAAAACCAGTTTTAGCATGGAGCAAGCCGAGGCTGTACT
  CGTGCACCACAGAATGGCCTTTGTCAGCATCTCACTGTTCACAGTTCGACTGTTGCAACTTCTTCTCCCT
  GTTGAAAAGGCGAGTGAGTTTTTGCCAGAGTCCATGTCAGCAGCATTATTTTTTGTCTCCTTGGACATGC
  CTATTTCTTTGGAATATCCAGAGATTCATGACACAGTTGTGGCTTATTTGGAACAGCTGGATTCTGAAAA
  TTACAGCATTTATAAGCGAACTGCAGAGGCTGTTTATTCAATTGAATGCACCTGTAACTTCCTATCTGAT
  GTGGGGAAAGAGGGAGAAAAGAATCTTCTAGAATTAGTGGAGCTGGCAGACCAAGCCTTGCGTAGCTTTT
  CCTATCATCAGCATTCTCCCCTAATAAAGGAAATCATCTGCATCTGTTCAAAGATCTGGAAATCTGCACA
  GGCTAGTCCATTACTCCAGGGAGAGAGTCAGAAGGTGTTTCTCCATATGCTGTCTCACCCATTGCCACAG
  GTGAAGGTGGAGACTTACCAGTGCTGCCTGGAGATTGTAAAGGAATGTTTAGGTGTCCAAAATGTCACTA
  AACCTGTGTCTTCACTTTGTAGTGGAATTCATTTTCTGCTTCATCCAAAAGTTCTGTATGAAATCTCTGC
  ATTTGGTATTCAAGAATCCAAAAATGAGGTGAATGCTGCTGCCAAGGCCATTCTGCTGTATCTGCTTCAG
  GGACGATTGATGATGACAGCTCTGACCTGGAACAAGTTTATCGAATCTCTCGCTCCTGTCATTCCAGTGC
  TACAGGGCTACGCTGATACAGAAGATCCCTTAGGCAACTGCATTCTCCTTCTAAGCAAAGTGAGCTCTGA
  GGCAGGAGAAGGCGCTCTCCCCTGCACTGCCCGGCTGAAGTCTGTGCTACGGCTTTTGCTAGTAAAAAAA
  CCTTCGGTTCGTTCACTAGCATTAAAACTTTTAGCATTTCATCTTACCAGTGAAGAAGGGGCTGATAAAA
  AGCGCCCGTTCATAGATGCTGGCGTTCTCTCCAGAGTTACTAATCTGTTTATTGTGAAAAAACCTATTGA
  CCTTAAGTTGGATGACAGAAGAGAACTAGTTATTAAGTTGGAGACTGTTAAAAAGGTGTGTGACATATTC
  ACCTCAGACGATGTTGATCTTGTTTTAAGAAAGTCAGCTGCAGAGCAACTAGCTGTGATAATGCAAGATA
  TTAAAATGCATACAGTGGTGAAAAAGTTATGCTTAGTTGAGAAGATTATTGGACATTTAAATGAATTTGT
  GGGTCAAGATGGTGAGGTTATAGAATGCTTGATTCAGCCATGCCTCACACTCCTGAGGAAGGTTTTGTAC
  GCTGACCCGGTTATCCGTGTTTCCTTGTCACAGCAGGCCTCTCTCTTGACCCTATTACTCAGAGTTTCCT
  TGATATTTCATGAAGATTGTACTGTGGTGGCTGAAGTCGGGGCATTGTTTTGTCTTCTATTGTTCGATGA
  AGTATCAAGAATGGATACATGGTCTGATACTCTTTCTGATAAGCCTTCTTTGTCATCAGTCTTCAGTTTG
  CCGGTTTCAGTCTTTAGAAGGTATCATCTACCAGTTCATGTGACTGGCCACCATGCTGTGAGTCCTTACT
  CAATAGTTTTGCCATTACCTACTGAATGTTTGACCCTGAAGCCGGTGTCAGATATGCTGAGAATAGCTTG
  GAACCTGGCATGGTATCATGGGAGTGATAATCTATTGAAACAAATGACCTCTGAAGCTAAAATTCAGGAA
  TTTTTAGATACACTGCAGCTGTCCACAGAAGATATCCTTGCACTGAAGATCACATACACGGATAGTGGGT
  TGAAGGACTGCCTTCAGTCCATCATTCAGGCTGGAGGCCACAGGGATGTGAGGGCTGCTATCACCAGGAT
  GAGTTTTTATCTTTTGAATGACAAACTGTCTTTGAAGGATGATATTGGTCCATGTGGCGTTACTTTAAAA
  TCCTTGGCTTGGCACACTGTGCTGAACAGGTTTTTACAAGTACTTCCTGCTTGCACTGAAGATGAGAAAC
  TGCTAATAGATATCATACATTTTTTGAATAAGTTAATAAAGGAACAAAGAAAAAACCCATCAATTGAACT
  TTTAAACTGGATTCTGGAATTACTTCTTAGGCATAATCCAAGTCCACTACTAGACCTGCTGGTTCTGACA
  GATTCACATGCAGGAGAAGAAACAGATGACGTCCGGACTGCTGTCAGACAGCAACTCCAGAAAGAACTGA
  TCAGTCTTCTCAATTCCTTGTTACTGGTTTTTATGACAGTCTCTGACAGGAAATGCTTGGAACTGTTTTA
  CGTTTTCCAAACACAGCTGTCTCTGAAGCTACTCCAGTGCCTCAGAGTCACAGATGCTCCCCATTTCTAT
  GGCTTACCTTCATTGGAGAGGACCTTAGCAGGGATGGCTCACCTCACTGCCTTCCCTGGGTGGAGTGCAC
  ACTCTCCTCTTTCAAAGCCACTTGAGATTTGTGGGAAGTACTTGTCCGGTCTCCTGGAGGTCATTACTTC
  CTTTTATGTTGAACGGGGAGGAAATGCTATGTCCTTCATGGGGAAAGGCGTTACAAAGAGCACAGGTCTT
  TGCTTACTTCACTTATCCCATGAGATGATAGTCCAGGCACCAGGCTCGGAGTGGATGCCGCTTTGGTTCT
  TGCCTTTGGGTAGTCACAGTGAAGAACATGCCCCTACTCAGCAAGGATTGGCCTGGCTAATTCCCTTATG
  GGTTGATAGAGACCCAGAGGTGAGATTCACTTCACTGGGGTTGGGATCAGCACTGACTACTATTGAAGCT
  GGCTGTGTGGCCTTGGCAAACAGTTGTCAGAATATTTCTGGTGGGCTCTGGGGAACCGTGGTGAACATTC
  TTCTGGATCAATCAGAATGCAGTCTGGTGCGCCGGGAGGCTGCATTTATTCTTCAGAATCTCCTTGTAAT
  TCCAATGCCTTCAGAAATTATAAAGGATTATACTTGGCAGGGCCCCTGTGTCCATGATGAGGATTCTGGC
  CTGTCACTTTCAGGAAAACCTGCCCTTCAGGCTCTTTTATATCACTGTCGTTTCTATGAACATTTGACTC
  ACATGGTGAAACATTGTTATGTAGGAAGATATATCTTTGATTTTAATTTTTCTGCTTTTACTGAAACTTC
  AGAATACAGTGATTTAAATGGTTTAGACGACTCATTCAAGTTTTGGAGGGCCCCTTCTGGGACATCTACT
  CAGGATCATGAGCCAAGCTCTCTTTCCACCTCAGAAACAATGGTGGCTGCCTCGGTGGTCAGTTCTGAGA
  TTCAGCCCCTGCTGCCGTCAACACTTCTTCCTGAAACTGCCCTTGACCAGTTTGTGACGCAAGGTCAACG
  TGAAATGAGACCAGTGCGACCTCGTGACTCATTGCTGTCTACTTCCCTGAACAGGCAGTATGTGTTTGTT
  ACTCCGTCTCTGCTGTCAGCAGTCTGTAGCCTTTTAGACAACCTCTTGGCAGTTACTCCAAGAGATGCTG
  CAAATGCTTTTCAACAAGCTCATCTCATAGAACTCCTCTGTAGCACGGTGGATGCTTCCCTCATAGAAAT
  GTGCATCCAAGAACTCAAGACCCCACTGCCTTTACCATCTGCTGTTGAACATATTCAGGCTCAGGCTTCC
  TTTTTCTTGGAATACCTATCCTCTTTGTCCCAGCTCTTGAAGTCCTGTCTGTTGGTAGAGCCTAAACTTG
  TGACACAAGATGAGCTCTTGAAACCTCTTATCACTAATGTCATCCGAGTTCTCATTGTGTGTCCCAAAGA
  TGTATTAGATGTACAGTTAGTGTTGGCTTTTTACCGCACATGGACGCATTTATTTGACCTTCTGGCCACA
  CTACTGAGGAAAGCTGGTGCTGTCTCCCTTGTTCCTCTTACCTCAGCTCTTGCCAAGCATTGGGAGGCAG
  TGACTGATACACTGTGTAGATGTGTGGGCCTCTCATTCAAGTGCCCTGCCCTGACTATTGCCAGCCTCCA
  GTTCCTGTCTGTTCTCTTGGCTGAGGAAGAAAAGAGGCGTGTCCAGGACAAGGACAAAACAAATGAGGGC
  CAGGCCCCAACAGTGGCCTTACTTCTTGATGGAACTCAGGGAAGCCTTAGCTCTTCAGAGCGACTTAATG
  AAACAATTCTTCAGTGCTATGAAGGAATATCCCCCAAAGATGTCCTGAAAAGAGTTGCTGCAAATGCCCT
  GTTGTCACTTCTGGCTGTTAGCAGAAGAGCGCAGAGGCATGCTTTGAGATCTGATCTCATAGAAAACTGT
  ATGGAGCAGATGAAGCACATCAATGCACAACTGAACTTAGATTTGCTGAGACCTGGGAAAGCAGTACTGA
  AAAAAAAGGAGGATGGCTTCATTAAAGAATTAAGCATCACCATGCAGCTCCTAAGAAACTGCCTTTATCA
  AAATGAAGAATGTAAAGTGGCAGCTCTTGAAGCACGTCTTGTGCCTGTATTGCACTCTCTCTGGCCTTGG
  CTTTTGATGGAAGATTCATTGATGCAAATTGCCTTGCAGTTGCTTTGTGTCTATACTGCAAATTTTCCAG
  CTGGCTGCAGTTCCCTTTGTTGGTCAAATTATGGACAGTGTATTCAAACTGCACACAGAGGCACCCCCAG
  TAGCTCCCTGATACTGTGTATCCTGAAACTGGCTTCTCAGATGCCTGCAGAGAACACCACCATTCAGCAG
  TTGGTTTTTATGTTTCTTTCAAACTTGGCCTTATCTCATGACTGCAAGGGAGTAATTCAAAAGAGCAACT
  TTCTACAGCACTTCTTGTCCCTGACATTGCCTAAAGGAGGAAACCAACATCTCAGCACTGTGGCCATTCT
  GTGGTTGAAGTTGCTCCTGAATATGTCATTTGGAGAAGATGGACAGCAAATGATCCTGAGGCTAGATGGC
  TGCTTAGACTTACTCATAGAGATGAGCAAGTACAAGCACAAGAGCAGCCCTCACATGCCTCTGCTGATCT
  TTCATAACATTTGCTTCAGTCCTGCTAATAAGCCTAAAATCCTGGCTAATGAAAAAGTTGTTACTCTTCT
  GGCTGCCTGTTTAGAAAGTGAGAATCAAACTGCCCAGAGGATTGGAGCAGCTTCACTTTGGGCTCTAACT
  TACAATTACCAAAAGGCAAAAGCAACTTTGAAAAATCCATCAATAAAAAGAAAAGTAGATGAAGCATATT
  CCATAGCAAAGAGAACTCTCTCAAACTCAGAAGAAAACCCTCTGAATTCCTACTATTTGAAATGCCTTGA
  AAATCTAGTACAACTGCTTAACTATTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175542
• Insert Size: 6681 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175542.3, NP_780751.2
• RefSeq Size: 7280 bp
• RefSeq ORF: 6681 bp
• Locus ID: 246102
• UniProt ID: Q8R4Y8
• Cytogenetics: 18 E4
• Gene Summary: Involved in the genetic cascade that governs left-right specification. Required for correct asymmetric expression of NODAL, LEFTY and PITX2 (By similarity).[UniProtKB/Swiss-Prot Function]