Cacna1c (NM_001159535) Mouse Untagged Clone

CAT#: MC225195

Cacna1c (untagged) - Mouse calcium channel, voltage-dependent, L type, alpha 1C subunit (Cacna1c), transcript variant 4, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cacna1c
• Synonyms: Cav1.2; Cchl1a1; D930026N18Rik; MBC; MELC-CC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225195 representing NM_001159535
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAATGAAAACACGAGGATGTACGTTCCAGAGGAAAACCACCAAGGTTCCAACTATGGGAGCCCAC
  GCCCAGCTCATGCCAACATGAATGCCAATGCAGCTGCAGGACTTGCTCCCGAGCACATCCCTACTCCAGG
  GGCAGCACTGTCCTGGCAGGCAGCCATCGATGCCGCCCGGCAGGCCAAGCTCATGGGCAGTGCTGGCAAC
  GCAACCATCTCTACCGTCAGTTCCACACAGCGGAAGCGGCAGCAGTATGGGAAACCCAAGAAGCAGGGGG
  GCACAACCGCCACACGGCCGCCCCGGGCTCTGCTGTGTCTGACCCTGAAGAACCCTATCCGGAGGGCGTG
  CATAAGCATTGTTGAATGGAAACCATTTGAAATCATTATTTTACTGACTATTTTTGCCAATTGTGTGGCC
  TTAGCAATCTATATTCCCTTTCCGGAAGACGACTCCAACGCCACCAACTCCAACCTGGAACGAGTGGAGT
  ATCTCTTCCTCATCATTTTTACCGTGGAAGCATTTTTAAAAGTAATTGCCTACGGACTTCTCTTCCACCC
  CAACGCTTACCTCCGCAATGGTTGGAATTTACTGGATTTTATAATCGTGGTTGTAGGGCTTTTTAGTGCA
  ATTTTAGAACAAGCAACCAAAGCTGATGGGGCCAATGCTCTAGGAGGGAAAGGAGCTGGATTCGACGTGA
  AGGCACTGAGAGCGTTCCGCGTGCTCCGTCCACTGCGGCTAGTGTCCGGAGTCCCAAGTCTCCAGGTGGT
  CCTGAACTCCATCATCAAGGCCATGGTGCCTCTGCTGCACATTGCCCTTCTTGTGCTCTTCGTCATCATC
  ATTTATGCTATTATCGGCCTGGAGCTCTTCATGGGAAAGATGCACAAGACCTGCTACAACCAGGAGGGCA
  TAATAGATGTTCCGGCAGAAGAGGATCCTTCCCCTTGTGCTTTGGAGACAGGCCATGGGCGACAGTGTCA
  GAACGGGACCGTGTGCAAACCCGGGTGGGATGGGCCCAAGCACGGCATCACCAACTTCGACAACTTCGCC
  TTCGCCATGCTGACGGTGTTCCAGTGTATCACCATGGAGGGCTGGACAGACGTGCTGTACTGGATGCAAG
  ACGCTATGGGCTATGAGTTGCCCTGGGTGTATTTTGTCAGTCTGGTCATCTTTGGATCCTTTTTCGTTCT
  AAATCTGGTTCTCGGTGTTTTGAGCGGGGAGTTTTCCAAAGAGAGGGAGAAAGCCAAAGCCCGAGGAGAT
  TTCCAGAAGCTTCGAGAGAAGCAGCAACTAGAAGAAGATCTCAAAGGCTACCTGGACTGGATCACCCAGG
  CAGAAGACATTGACCCCGAGAATGAGGACGAGGGCATGGATGAAGACAAGCCTCGAAACATGAGCATGCC
  CACAAGTGAGACTGAGTCTGTCAACACTGAAAACGTGGCTGGAGGTGACATCGAGGGAGAAAACTGTGGA
  GCCAGGCTTGCCCATCGGATCTCCAAATCCAAATTCAGCCGCTACTGGCGCAGGTGGAATCGATTCTGCA
  GAAGAAAATGCCGTGCAGCAGTTAAGTCCAACGTCTTCTACTGGCTCGTGATCTTCCTGGTGTTCCTCAA
  CACCCTCACCATTGCCTCCGAACATTACAACCAGCCTCACTGGCTCACAGAAGTGCAAGACACAGCCAAT
  AAAGCCCTCCTGGCCCTTTTCACTGCAGAAATGCTCCTGAAGATGTACAGCCTGGGTCTTCAGGCCTATT
  TTGTGTCCCTCTTCAACCGCTTTGACTGTTTCATTGTGTGTGGGGGCATCCTGGAGACCATCCTGGTGGA
  GACGAAGATCATGTCTCCCCTGGGCATCTCTGTGCTGAGATGTGTGCGGTTGCTCAGGATCTTCAAGATC
  ACCAGGTACTGGAATTCCTTGAGCAACCTTGTGGCATCCTTGCTGAACTCAGTGCGCTCCATTGCCTCCC
  TGCTGCTGCTCCTCTTCCTCTTCATCATCATCTTCTCCCTCCTGGGGATGCAGCTCTTTGGAGGGAAGTT
  CAATTTCGATGAGATGCAGACCCGTAGGAGCACGTTCGATAACTTCCCGCAGTCTCTCCTCACTGTGTTT
  CAGATCCTGACCGGGGAGGACTGGAATTCGGTGATGTATGATGGGATCATGGCTTATGGCGGCCCCTCTT
  TTCCAGGGATGTTAGTCTGTATTTACTTCATCATCCTCTTCATCTGTGGAAATTATATCCTACTGAATGT
  GTTCTTGGCCATTGCGGTGGACAACCTGGCTGATGCGGAGAGCCTGACCTCAGCCCAAAAGGAGGAGGAA
  GAAGAGAAGGAGAGGAAGAAGCTGGCCAGGACTGCCAGCCCAGAAAAGAAACAGGAGGTGATGGAGAAGC
  CAGCCGTGGAGGAGAGCAAAGAGGAGAAAATTGAACTGAAATCCATTACAGCCGATGGAGAATCCCCACC
  CACTACCAAGATCAACATGGATGACCTCCAGCCCAGTGAAAACGAGGATAAGAGTCCCCACTCCAACCCA
  GACACTGCAGGGGAAGAGGATGAAGAGGAGCCAGAGATGCCTGTGGGGCCACGCCCCCGGCCCCTGTCTG
  AGCTGCACCTTAAGGAAAAGGCAGTTCCCATGCCGGAAGCCAGTGCATTTTTCATCTTCAGCCCAAACAA
  CAGGTTCCGCCTGCAGTGCCACCGTATTGTCAATGACACGATCTTCACCAACCTCATCCTCTTCTTCATT
  CTGCTCAGCAGCATCTCTCTGGCTGCTGAGGACCCCGTCCAGCACACCTCCTTCAGGAACCATATCCTAG
  GCAATGCAGACTATGTCTTCACTAGTATCTTTACATTAGAAATTATCCTTAAGATGACTGCTTACGGGGC
  TTTCCTGCACAAGGGCTCTTTCTGCCGAAACTACTTCAATATCCTGGACCTGCTGGTGGTTAGCGTGTCC
  CTCATCTCCTTTGGCATCCAGTCCAGCGCGATCAACGTTGTGAAGATTTTACGAGTGCTGCGAGTCCTCA
  GACCCCTGAGGGCCATCAACAGGGCCAAGGGGCTAAAGCATGTGGTTCAGTGCGTGTTTGTGGCCATCCG
  GACCATCGGGAACATCGTAATTGTCACCACTCTGCTGCAGTTCATGTTCGCCTGCATTGGGGTCCAGCTC
  TTCAAGGGAAAGCTCTATACCTGTTCGGATAGTTCTAAACAGACGGAGGCAGAATGCAAGGGTAACTATA
  TCACATACAAAGATGGAGAGGTCGATCACCCCATTATCCAGCCTCGAAGCTGGGAGAACAGCAAGTTTGA
  CTTTGACAATGTTTTGGCAGCCATGATGGCTCTCTTCACCGTCTCCACCTTCGAAGGGTGGCCAGAGCTG
  CTGTACCGCTCCATTGACTCCCACACAGAAGACAAGGGCCCCATCTACAACTACCGTGTGGAGATCTCCA
  TCTTCTTCATCATCTATATCATCATCATTGCCTTCTTCATGATGAACATCTTCGTGGGTTTCGTCATTGT
  CACCTTCCAGGAGCAGGGGGAACAAGAGTACAAGAACTGTGAGCTGGACAAGAACCAGAGACAATGTGTG
  GAATATGCCCTCAAGGCCCGACCCTTGCGAAGGTACATCCCCAAGAACCAGCACCAGTACAAAGTGTGGT
  ACGTGGTCAACTCTACCTACTTCGAGTATCTGATGTTCGTTCTCATCCTGCTCAACACCATCTGCCTGGC
  CATGCAGCACTATGGCCAGAGCTGCCTCTTCAAAATCGCCATGAATATACTCAACATGCTTTTCACCGGC
  CTCTTCACAGTGGAGATGATCCTGAAGCTCATTGCCTTCAAACCCAAGCACTATTTCTGTGATGCATGGA
  ATACATTTGACGCCTTGATTGTTGTGGGTAGCATTGTTGATATAGCAATCACCGAGCCAGCTGAACATAC
  CCAATGCTCTCCCTCTATGAGTGCAGAGGAGAACTCCCGCATCTCCATCACCTTCTTCCGCCTCTTCCGG
  GTCATGCGCCTGGTGAAGCTGCTGAGCCGCGGGGAAGGCATCCGAACCCTGCTGTGGACCTTCATCAAGT
  CCTTCCAGGCTCTGCCCTATGTGGCTCTTTTGATTGTGATGCTGTTCTTTATCTATGCAGTGATTGGGAT
  GCAGGTGTTTGGGAAGATTGCCCTGAATGACACCACAGAGATCAATCGGAACAACAACTTCCAGACGTTC
  CCCCAGGCTGTGTTACTGCTGTTCAGGTGTGCCACCGGAGAGGCCTGGCAGGACATCATGCTGGCCTGCA
  TGCCAGGCAAGAAGTGTGCCCCAGAGTCTGAGCCCAGCAACAGCACGGAAGGGGAGACCCCCTGTGGCAG
  CAGCTTTGCTGTCTTCTACTTCATCAGCTTCTACATGCTCTGTGCCTTCCTGATCATCAACCTCTTTGTA
  GCTGTTATCATGGACAACTTTGACTACCTGACTAGGGATTGGTCTATCCTCGGTCCCCATCACCTGGATG
  AATTCAAGAGAATCTGGGCCGAGTATGACCCTGAAGCCAAGGGTCGGATCAAACACTTGGATGTGGTGAC
  CCTCCTCCGTCGAATTCAGCCCCCACTGGGTTTTGGGAAATTGTGTCCTCACCGTGTGGCCTGCAAACGC
  CTGGTGTCCATGAACATGCCTCTGAACAGCGATGGCACAGTCATGTTCAATGCTACCCTGTTTGCCCTCG
  TCAGGACAGCCCTGAGGATCAAAACAGAAGGGAACCTAGAGCAAGCCAATGAGGAGCTTCGGGCCATCAT
  CAAGAAAATCTGGAAGAGGACTAGCATGAAGCTGTTGGACCAGGTGGTGCCCCCTGCAGGCGATGACGAG
  GTCACAGTGGGCAAGTTCTATGCCACCTTCCTGATCCAAGAGTACTTCAGGAAATTCAAGAAGCGAAAAG
  AGCAGGGGCTGGTGGGCAAGCCCTCACAAAGGAATGCACTGTCCCTCCAGGCTGGCTTGCGCACCTTGCA
  TGACATTGGGCCTGAGATCCGGCGGGCCATCTCTGGGGATCTGACTGCTGAGGAGGAGTTGGACAAGGCT
  ATGAAGGAGGCGGTGTCTGCTGCCTCCGAAGATGACATCTTCAGGAGGGCTGGAGGCCTGTTCGGCAACC
  ATGTCACCTACTATCAGAGTGACAGCAGGGGCAACTTTCCTCAGACGTTCGCCACCCAGCGCCCACTGCA
  CATCAACAAGACAGGGAACAACCAAGCTGACACTGAGTCACCGTCCCATGAGAAGCTGGTGGACTCCACG
  TTCACCCCCAGCAGCTACTCATCCACGGGCTCCAATGCCAACATCAACAATGCCAACAACACTGCCCTGG
  GCCGCTTCCCCCATCCCGCTGGCTACTCCAGCACGGTCAGCACTGTGGAGGGCCATGGGCCTCCCTTGTC
  CCCTGCTGTCCGAGTACAGGAGGCAGCATGGAAGCTCAGCTCTAAGAGGTGCCACTCCCGAGAGAGCCAG
  GGAGCCACGGTGAATCAGGAGATATTTCCAGATGAGACCCGCAGCGTAAGGATGAGTGAAGAAGCCGAGT
  ACTGCAGTGAGCCCAGCCTGCTCTCCACAGATATGTTCTCCTACCAGGAAGATGAACACCGACAACTGAC
  CTGCCCAGAGGAGGACAAGAGGGAGATCCAGCCATCTCCAAAGAGGAGTTTCCTTCGCTCTGCCTCTCTA
  GGTCGAAGGGCCTCCTTCCATCTGGAATGTCTAAAGCGACAAAAGGATCAAGGGGGAGACATCTCTCAGA
  AGACAGCCTTGCCCTTGCATCTGGTTCATCATCAGGCATTGGCAGTGGCAGGCTTGAGCCCCCTCCTGCA
  GAGAAGCCATTCTCCTACCACATTCCCCAGGCCGTGCCCCACACCCCCTGTCACTCCAGGCAGCCGGGGC
  AGACCCCTACGGCCCATCCCTACCCTACGGCTGGAGGGGGCAGAGTCCAGCGAGAAACTCAACAGCAGCT
  TCCCATCCATCCACTGCAGCTCCTGGTCTGAGGAGACGACAGCCTGTAGTGGGAGCAGCAGCATGGCCCG
  GAGAGCCCGGCCCGTCTCCCTCACCGTGCCCAGCCAGGCTGGAGCTCCAGGGAGACAGTTCCATGGCAGT
  GCCAGCAGCCTGGTGGAAGCGGTCTTGATTTCAGAAGGACTGGGACAGTTTGCTCAAGATCCCAAGTTCA
  TCGAGGTCACCACCCAGGAGCTGGCTGACGCCTGCGACATGACAATAGAGGAGATGGAGAACGCCGCAGA
  CAACATCCTCAGTGGGGGCGCCCAGCAGAGCCCCAACGGCACCCTCTTACCTTTTGTGAACTGCAGGGAC
  CCGGGGCAGGACAGGGCTGTGGCCCCAGAGGACGAGAGCTGCGCATATGCCCTGGGGCGAGGCCGGAGCG
  AGGAGGCGCTCGCGGACAGCAGGTCCTACGTCAGCAACCTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159535
• Insert Size: 6414 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159535.2, NP_001153007.1
• RefSeq Size: 13586 bp
• RefSeq ORF: 6414 bp
• Locus ID: 12288
• Cytogenetics: 6 55.86 cM
• Gene Summary: Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:14609949, PubMed:18586882, PubMed:21216955, PubMed:25368181, PubMed:28119464). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed:21216955). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed:14609949, PubMed:28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) has an alternate 5' exon and an alternate internal exon in the CDS, as compared to variant 1. The resulting isoform (4) has a shorter and different N-terminus and a shorter and different internal segment, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.