Scn5a (NM_021544) Mouse Untagged Clone

CAT#: MC225126

Scn5a (untagged) - Mouse sodium channel, voltage-gated, type V, alpha (Scn5a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Scn5a
• Synonyms: mH1; Nav1.5; Nav1.5c; SkM1; SkM2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225126 representing NM_021544
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAACTTCCTGTTACCTCGGGGCACCAGCAGCTTCCGTAGGTTCACCCGGGAGTCACTGGCGGCCA
  TCGAGAAGCGCATGGCTGAAAAACAAGCCCGTGGTTCGGCCACCTCACAGGAGAGCCGTGAGGGCCTGCC
  AGAGGAGGAGGCTCCCCGGCCCCAGCTGGACCTACAGGCCTCCAAAAAGCTGCCAGATCTCTATGGCAAC
  CCACCCCGAGAGCTCATTGGGGAGCCCCTGGAAGACCTGGACCCTTTCTATAGTACCCAGAAGACCTTCA
  TCGTGCTGAATAAGGGCAAAACCATCTTCCGGTTCAGTGCCACCAATGCCTTGTACGTCCTCAGCCCCTT
  CCACCCGGTGCGCAGAGCGGCTGTGAAGATTTTGGTACATTCGCTCTTCAGCATGCTCATCATGTGCACC
  ATCCTAACCAACTGTGTGTTCATGGCACAACACGACCCTCCGCCTTGGACCAAATATGTTGAGTACACCT
  TCACCGCCATCTACACCTTTGAGTCTCTGGTCAAGATTCTAGCTCGAGGCTTCTGCCTGCATGCGTTCAC
  CTTCCTCCGGGACCCGTGGAACTGGCTAGACTTCAGTGTGATCGTCATGGCGTATGTATCAGAGAATATA
  AAGCTAGGCAATTTGTCGGCTCTTCGAACTTTCAGAGTCCTGAGAGCTCTGAAAACGATTTCAGTTATTC
  CAGGCCTGAAGACAATCGTGGGAGCCCTAATCCAGTCTGTGAAGAAGCTAGCCGATGTGATGGTCCTTAC
  TGTCTTCTGCCTCAGTGTCTTTGCCCTCATTGGCCTGCAGCTCTTCATGGGCAACCTGAGGCACAAGTGC
  GTGCGTAACTTTACTGAGCTCAATGGCACCAATGGTTCCGTGGAGGCCGATGGCATAGTCTGGAACTCTC
  TGGACGTCTACCTCAATGACCCAGCCAATTACCTACTCAAGAATGGCACCACGGATGTGTTGCTGTGTGG
  GAACAGCTCTGATGCCGGGACATGTCCTGAAGGCTACCGGTGCCTGAAGGCAGGTGAGAACCCTGACCAC
  GGTTACACCAGCTTCGACTCCTTCGCCTGGGCTTTCCTTGCGCTCTTCCGCCTGATGACACAGGACTGCT
  GGGAACGTCTATACCAGCAGACCCTGAGGTCCGCAGGAAAGATCTACATGATCTTCTTCATGCTTGTCAT
  ATTCCTGGGCTCCTTCTACCTTGTGAACTTGATCCTGGCTGTGGTGGCCATGGCTTACGAGGAGCAAAAC
  CAAGCCACCATCGCCGAGACGGAAGAGAAGGAGAAGCGTTTCCAGGAGGCCATGGAGATGCTCAAGAAGG
  AACACGAGGCTCTCACCATCAGGGGCGTGGATACTGTGTCCCGTAGCTCTCTGGAGATGTCTCCTTTGGC
  CCCAGTAACCAACCATGAGAGAAGGAGCAAGAGGAGGAAACGACTGTCTTCAGGGACAGAGGATGGCGGA
  GATGACAGGCTCCCCAAGTCGGACTCAGAAGACGGTCCCAGAGCATTGAATCAGCTCAGCCTCACCCATG
  GGCTCAGCCGGACATCCATGAGGCCCCGCTCCAGCCGAGGGAGCATTTTCACATTCCGGCGACGGGACCA
  AGGCTCCGAGGCAGATTTTGCAGATGACGAGAACAGTACAGCAGGAGAGAGCGAGAGCCACCGCACATCG
  CTGCTGGTGCCCTGGCCCCTGCGCCGTCCCAGCACCCAAGGGCAGCCCGGCTTTGGAACCTCAGCTCCTG
  GTCATGTCCTCAATGGCAAAAGAAACAGCACCGTGGACTGCAATGGGGTGGTTTCCTTGCTGGGGGCAGG
  GGACGCAGAGGCCACCTCTCCAGGGAGCCACCTTCTCCGCCCTATAGTGCTGGACAGACCCCCAGACACG
  ACCACGCCGTCAGAGGAGCCGGGTGGGCCCCAGATGCTGACACCGCAGGCTCCATGTGCAGATGGCTTTG
  AGGAGCCCGGGGCACGGCAACGGGCACTCAGCGCCGTCAGTGTCCTCACCAGCGCTCTGGAAGAGTTGGA
  GGAGTCCCACCGTAAGTGTCCACCATGCTGGAATCGCTTTGCCCAGCATTACCTCATCTGGGAGTGCTGC
  CCACTCTGGATGTCCATCAAGCAGAAGGTGAAGTTCGTGGTCATGGACCCGTTTGCTGACCTCACCATCA
  CCATGTGCATCGTGCTCAACACGCTCTTCATGGCTCTGGAGCACTACAACATGACAGCCGAGTTTGAGGA
  GATGCTGCAGGTCGGAAACTTGGTCTTCACGGGAATCTTCACGGCAGAGATGACCTTCAAGATCATCGCC
  CTTGACCCATACTACTACTTCCAGCAGGGCTGGAATATCTTCGACAGCATCATCGTCATCCTCAGCCTCA
  TGGAGCTGGGCCTGTCTCGCATGGGCAACCTGTCTGTGCTGCGTTCCTTCCGTCTGCTGCGGGTCTTCAA
  GCTGGCCAAATCATGGCCCACCCTGAACACACTCATCAAGATCATCGGGAACTCTGTTGGTGCCCTGGGG
  AACCTGACCCTGGTGCTGGCCATCATCGTCTTCATCTTCGCCGTGGTGGGTATGCAGCTCTTCGGCAAGA
  ACTACTCAGAGCTGAGGCACCGAATCAGCGACTCTGGCCTGCTGCCCCGCTGGCACATGATGGACTTCTT
  CCACGCCTTCCTCATCATCTTCCGCATCCTCTGTGGAGAGTGGATCGAGACCATGTGGGACTGCATGGAG
  GTGTCTGGGCAGTCGCTGTGCTTGCTGGTCTTCCTGCTGGTCATGGTCATTGGCAACCTTGTGGTCCTGA
  ATCTCTTCTTGGCCTTGCTGCTCAGCTCCTTCAGCGCAGACAACCTCACAGCCCCTGACGAGGATGGGGA
  GATGAACAACCTCCAGCTGGCCCTGGCTCGCATCCAAAGGGGCCTTCGCTTTGTCAAGCGGACCACCTGG
  GACTTCTGCTGCGGGCTCCTGCGGCGGCGACCTAAGAAGCCCGCGGCTCTTGCCACCCACAGCCAGCTGC
  CCAGCTGTATCGCCGCTCCCAGGTCCCCACCACCCCCAGAGGTGGAGAAGGCGCCCCCAGCCCGCAAGGA
  AACACGGTTCGAGGAAGACAAGCGGCCGGGCCAGGGCACCCCTGGGGATACCGAGCCTGTGTGTGTTCCC
  ATCGCAGTGGCTGAGTCAGACACTGATGACCAGGAAGAGGATGAGGAGAACAGCCTTGGCACGGAGGAGG
  AAGAGTCCAGCAAACAGCAGGAATCCCAAGTTGTGTCTGGTGGCCACGAGCCCCCCCAGGAGCCCAGGGC
  CTGGAGCCAGGTGTCAGAGACCACATCCTCTGAAGCCGAGGCCAGTACGTCTCAGGCAGACTGGCAGCAA
  GAGCGGGAAGCGGAGCCCCGGGCCCCGGGGTGCGGTGAGACTCCCGAGGACAGTTACTCCGAGGGTAGCA
  CCGCTGACATGACCAACACCGCAGACCTCCTGGAGCAAATCCCAGACCTCGGGGAGGACGTCAAGGACCC
  AGAGGACTGCTTCACTGAAGGCTGCGTCCGACGTTGTCCTTGCTGCATGGTGGACACGACGCAGGCCCCA
  GGGAAGGTCTGGTGGAGACTGCGCAAGACCTGCTACCGCATAGTGGAGCACAGCTGGTTCGAGACTTTCA
  TCATCTTCATGATCCTGCTCAGCAGCGGAGCGCTGGCCTTCGAGGACATCTACCTGGAGGAGCGGAAGAC
  CATCAAGGTTCTGCTGGAGTACGCCGACAAGATGTTCACCTATGTCTTTGTGTTGGAGATGCTGCTCAAG
  TGGGTGGCCTACGGCTTCAAGAAGTACTTCACCAACGCCTGGTGCTGGCTGGACTTCCTAATTGTGGACG
  TCTCACTGGTCAGCCTCGTGGCCAACACCTTAGGCTTTGCCGAGATGGGTCCCATCAAGTCCCTGAGGAC
  ACTGCGTGCACTTCGACCCCTGAGGGCTTTGTCACGATTCGAGGGCATGAGGGTGGTGGTCAACGCGCTG
  GTGGGCGCCATCCCCTCCATCATGAATGTCCTCCTCGTCTGCCTCATCTTCTGGCTCATCTTCAGCATCA
  TGGGCGTGAACCTCTTCGCCGGGAAGTTCGGTAGGTGCATCAACCAGACCGAAGGGGACCTGCCTCTGAA
  CTACACCATTGTGAACAACAAGAGCGAGTGTGAGTCCTTCAACGTGACTGGAGAGTTGTACTGGACCAAG
  GTGAAAGTCAACTTTGACAACGTGGGGGCTGGGTACCTGGCCCTGCTGCAGGTGGCGACATTTAAAGGCT
  GGATGGACATCATGTATGCGGCTGTGGACTCCAGAGGGTATGAAGAGCAGCCGCAGTGGGAGGACAACCT
  CTACATGTACATCTACTTCGTCGTCTTCATCATCTTCGGCTCCTTCTTCACCCTCAACCTGTTTATTGGT
  GTCATCATTGATAACTTCAACCAGCAGAAGAAAAAGTTAGGGGGCCAGGACATCTTCATGACGGAGGAGC
  AGAAGAAGTACTACAATGCCATGAAGAAGCTGGGCTCCAAGAAACCCCAGAAGCCCATCCCACGGCCTCT
  GAACAAGTACCAGGGTTTCATATTCGACATTGTGACCAAGCAGGCCTTCGATGTTACCATCATGTTCCTC
  ATCTGTTTAAACATGGTGACCATGATGGTAGAGACAGATGATCAGAGCCCTGAAAAAGTCAACATCTTGG
  CCAAGATCAACCTGCTCTTCGTGGCCATCTTCACAGGAGAGTGTATTGTCAAGATGGCTGCCCTGCGCCA
  CTATTACTTCACCAACAGCTGGAACATCTTCGATTTCGTGGTTGTCATCCTCTCCATCGTTGGCACTGTC
  CTCTCCGACATCATCCAGAAGTACTTCTTCTCCCCAACGCTCTTCCGTGTCATCCGTCTGGCCAGGATCG
  GCCGCATCCTCAGGCTGATCCGAGGAGCCAAGGGGATTCGCACGTTGCTCTTCGCCCTCATGATGTCCCT
  GCCCGCCCTCTTCAACATCGGCCTCCTCCTCTTCCTCGTCATGTTCATCTACTCCATCTTCGGCATGGCC
  AACTTTGCCTACGTCAAGTGGGAGGCTGGCATCGACGACATGTTCAACTTCCAGACCTTTGCCAACAGCA
  TGCTGTGCCTTTTCCAGATCACCACGTCGGCTGGCTGGGATGGCCTCCTCAGCCCCATCCTCAACACAGG
  ACCCCCCTACTGTGACCCCAACCTGCCCAACAGCAATGGCTCCAGGGGGAACTGTGGGAGCCCAGCAGTG
  GGCATCCTCTTCTTCACCACCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACATTGCCATCA
  TCCTGGAGAACTTCAGTGTGGCCACAGAAGAGAGCACAGAGCCCCTGAGCGAGGATGACTTCGACATGTT
  CTATGAGATCTGGGAGAAGTTCGACCCGGAGGCCACCCAGTTCATTGAGTATTTGGCCCTGTCCGACTTT
  GCCGATGCCCTGTCTGAGCCACTCCGCATCGCCAAGCCCAACCAGATAAGCCTCATCAATATGGACCTGC
  CCATGGTGAGCGGGGACCGCATCCACTGTATGGACATCCTGTTCGCCTTCACCAAGAGGGTGCTCGGGGA
  GTCTGGGGAGATGGATGCCCTGAAGATCCAGATGGAGGAGAAATTCATGGCGGCCAATCCTTCCAAGATC
  TCCTATGAGCCCATCACCACCACCCTGCGGCGAAAACATGAGGAGGTGTCGGCCACGGTCATCCAGCGGG
  CCTTCCGGAGGCACCTCCTGCAGCGCTCGGTGAAGCATGCTTCCTTCCTCTTCCGCCAGCAAGCGGGCAG
  CAGTGGCCTCTCTGATGAGGATGCCCCCGAGCGAGAGGGACTCATTGCCTACATGATGAATGAGAACTTC
  TCCCGGCGCAGCGGTCCCCTCTCCAGCTCTTCCATCTCCTCCACGTCCTTCCCACCGTCCTATGACAGCG
  TCACGAGGGCCACCAGTGATAACCTCCCAGTGCGTGCGTCGGACTACAGCCGCAGCGAAGATCTTGCAGA
  CTTCCCTCCATCTCCAGATAGGGACCGAGAGTCTATAGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021544
• Insert Size: 6063 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021544.4, NP_067519.2
• RefSeq Size: 8455 bp
• RefSeq ORF: 6063 bp
• Locus ID: 20271
• UniProt ID: Q9JJV9
• Cytogenetics: 9 71.33 cM
• Gene Summary: This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:11834499, PubMed:23420830). It is a tetrodotoxin-resistant Na(+) channel isoform. This channel is responsible for the initial upstroke of the action potential. Channel inactivation is regulated by intracellular calcium levels (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1).