Crocc (NM_001145958) Mouse Untagged Clone

CAT#: MC224969

Crocc (untagged) - Mouse ciliary rootlet coiled-coil, rootletin (Crocc), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Crocc
• Synonyms: KIAA0445
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224969 representing NM_001145958
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCACTGCTGTCGCTGCAGGAGGAAAACCAGCTGCTGCAGCAGGAGCTGTCCCGCGTGGAGGACC
  TGCTGGCCCAGAGCCGCGCAGAGCGGGATGAGCTGGCCATCAAGTACAATGCTGTCAATGAGAGGGACAC
  AGAACACAGCCAGGACCTGGACAGCGCTCTTCTGCGCCTAGAGGAGGAACAGCAGAGAAGTGCCAGCCTG
  GCCCAGGTGAATGCCATGCTGAGAGAGCAGCTGGACCAGGCCAACTTGGCCAACCAGGCTCTGAGCGAGG
  ACATACGCAAGGTGACCAGTGACTGGACCCGCAGCTGTAAGGAGCTGGAGCAGCGGGAGGCAGTGTGGAG
  GCGCGAGGAAGAGTCCTTCAACACCTACTTCAGCAGCGAGCACAGCCGCCTGCTCCGCCTCTGGAGGCAG
  GTCATGGGGCTCCGACGGCAGGCCAGCGAGGTGAAGATGGGCACGGAGAGGGACTTGTTGCAGCTGGGCG
  GGGAGCTCGTCCGCACATCCCGGGCTGTCCAGGAGCTGGGCCTCGGACTGAGTGCCAGCCTGCATCGGGC
  TGAGAGCAAGGCAGAGGCAGCTCTGGAGAAGCAAAAGCTGCTGCAGGCCCAACTAGAGGAGCAGCTACAG
  GCCAAGCTGCTCCGGGAGAAGGACCTGGCCCAACTGCAGGTGCAGAGTGACCTGGACAAGGCTGATCTCA
  GTGCCAGAGTGACAGAGCTGGCCCTGTCCGTGGAGCACCTTCAGAATCAGAACTCAGAGAAGGACCAGGT
  CAACAGGACCCTCTCTGACAAGCTGGAGGCCCTGGAGTCACTGCGGCTTCAGGAACAGACAACCCTGGAC
  ACTGAGGATGGAGAGGGGCTGCAGCAGACCCTGAGGGACCTGGCACAGGCTGCCCTATCTGACACTGAGA
  GTGGTGTGCAGCTCAGCAGCTCAGAGCGCACTGCGGACACCTCTGACGGCAGCCTGCGCGGGTTCTCTGG
  CCAGCGGACTCCAACTCCACCGCGGCATTCTCCGGGTCGAGGTCGTTCTCCGCGCCGAGGCCTGTCCCCA
  GCCTGCTCCGACTCCTCCACACTCACGCTGATCCACTCAGCCCTGCACAAGCGCCAGCTACAGGTTCAGG
  ACATGCGTGGACGCTATGAAGCCAGCCAAGAACTGCTGGGTTCCGTACGCAAGCAGCTCAGTGACAGTGA
  GGGCGAGCGGCGTGGCCTGGAGGAGCAGCTACAGCGCCTGCGGGACCAGACAGCAGCCTCAGCCCAGGCC
  CAAGAGGATGCGCAGCGCGAGGCCCAGCGCCTACGCAGTGCCAACGAGCTCCTGAGCAGGGAGAAGGGGA
  ACCTGACCCACAGCCTGCAGGTGACCCAGCAGCAAGCTAAGGAGCTGCGCCAGGAGCTGGAGAAGCTGCA
  GGCTGCCCAGGAGGAGCTGAAACGTCAGCATAACCAACTGGAGGACGCGCAGGAGGACTCGGTGCAGGAG
  GGCGCCCGGGCCCGCCGGGAGCTGGAGCGCAGCCATAGGCAGCTGGAACAGCTGGAGGTGAAGCGCTCAG
  GGCTGACTAAGGAGCTGGTGGAGGTGCGGGAGGCACTGAGCTGCGCCATACTGCAGAGGGATGTACTGCA
  GACGGAGAAGGCAGAGGTGGCCGAGGCGCTAACCAAGGCTGAAGCAGGCCGAGCGCAGCTGGAGCTCTCC
  CTGACCAAGCTGAGGGCAGAGGAGGCCTCCCTGCGAGACTCCTTATCCAAGATGAGTGCCCTGAATGAGA
  GCCTCGCCCAGGACAAGCTGGAATTAAACCGCCTTATCGCCCAGCTAGAGGAAGAAAAGGTGGCACTCCT
  GGGCCGCCAGCAGCAGGCAGAACATGCCACTACGATGGCCGTGGAGAAGCAGGAACTTTTGGAGCAGCTG
  AGGCTGGAGCAGGAGGTGGAGAGGCAGGGCCTACAGGGCTCCCTGTGTGTGGCTGAGCAGGCCCGGGAGG
  CGCTGGAACAACAGATCCTTGTACTGCGCAGCGAACGCAGCCATCTGCAGGAGCAGTTGGCCCAGCTCTC
  CCGGCAGCTGAGTGGACGGGACCAGGAGCTGGAGCAAGCCCTGCGGGAGTCCCAGCGGCAGGTGGAAGCA
  CTGGAGCGCGCTGCCCGGGAGAAGGAGGCTATGGCCAAGGAGCGGGCTGGCCTGGCAGTGAAGCTGGCAG
  CAGCAGAGCGGGAGGGCCGGACCCTGTCAGAGGAGGCCATTCGCCTGCGCCTGGAGAAGGAGGCCCTGGA
  GAGCAGCCTATTTGACGTGCAGAGGCAGCTGGCTCAGCTCGAGGCCCGCCGAGAGCAGCTAGAAGCCGAC
  AGTCAAGCCCTGCTGCTGGCCAAGGAAACTCTGACTGGGGAGCTGGCAGGCCTACGGCAGCAGGTAACAT
  CCACAGAAGAGAAGGCAGCCTTGGACAAGGAGCTGATGACCCAGAAGCTAGTGCAAGCCGAGCGGGAGGC
  ACAGGCCTCTCTGCGGGAGCAGCGGGCAGCCCATGAGGAGGATCTCCAGAGACTGCAGCATGAGAAGGAG
  GCTGCATGGCGGGAGCTTCAGGCAGAGCGGGCCCAGCTGCAGGGCCAGCTGCAGCAGGAGCGAGAGGAGC
  TGCTGGCACGGATGGAGGCCGAGAAGGAGGAGCTGAGCAAGGAGATTGCCGCGCTGCAGCAGGAGCGGGA
  CGAGGGCCTGCTGCTGGCAGAGAGCGAAAAGCAGCAGGCCTTGTCCCTGAAGGAGTCCGAGAAGACGGCG
  CTGTCGGAGAAGTTGATGGGAACTCGGCACAGTCTGGCTGCCATCTCTCTGGAGATGGAACGGCAGAAGC
  GAGATGCCCAGAGCCGGCAGGAACAGGACCGGAACACACTGAACGCCCTGACATCTGAACTTCGAGACCT
  CCGGGCCCAGCTGGAGGAAGCCACTGCCGCCCATGCCCAGACGGTGAAAGAGCTGGAGGAACGGACGGGG
  AACCTGGGCCGGCAGCGGGAGGCCTGCATGCGGGAGGCAGAAGAGCTGAGGACTCAGCTGCGTGTGTTGG
  AGGACACCCGCGATGGGCTGCGGCGGGAGCTGCTGGAGGCTCAGCGTAAGGGTCGGGACAGTCAGGACAG
  CTCCGAAGCCCATCGCCAAGAGGCTAGTGAGCTGCGGCGCAGCCTGAGTGAGGGCGCCAAGGAGCGGGAG
  GCCCTGCGGCGGTCCAACGAGGAGCTGAGGTCTGCAGTGAAGAAGGCGGAGAGTGAGCGCATCAGCCTGA
  AGCTTGCCAATGAGGACAAGGAGCAGAAGTTGGCCCTCCTGGAAGAGGCTCGAGTGTCTGTAGCCAAGGA
  GGCTGGAGAGCTGCGGGCCTCGCTGCAGGAGGTGGAGCGATCCCGGCTGGAGGCTCGACGTGAGCTGCAG
  GAGCTTAGGCGACAGATGAAGACACTGGACAGTGACAATGGCAGGCTGGGCCGGGAGCTGGCAGACCTGC
  AGGGCCGCTTGGCCCTGGGTGAGCGGACGGAGAAGGAGAGCAGGCGAGAGGCCCTGGGCCTACGGCAGAG
  GTTGCTGAAAGGCGAGAGCAGCCTGGAAGCCCTGAAACAGGAGCTCCAGGGTTCCCAGAGGAAGCTGCAG
  GAGCAAGAGGCCGAGTTCCGGGCGCGGGAGCGAGGCCTGTTGGGCTCCCTGGAGGAGGCGCGTGGTGCCG
  AGAAGAGGCTTCTGGACTCTGCCCGCAGCCTGGAGCTGAGGCTGGAGGCGGTGCGGGCAGAGACCTCAGA
  GCTGGGGCTGCGGCTGAGCGCGGCCGAGGGCCGGGCGCAGGGTCTGGAGGTCGAGCTTGCCCGCGTGGAG
  GCGCAGCGGCGGGTGGCCGAGGCCCAGCTAGGCGGCCTGCGCTCCGCCCTGCGCCGGGGCCTAGGCCTGG
  GCCGAGTGTCCAGCTCCCCAGCCCGGGAGGCGCCTGCTGGAGGAAGTGGGGATGGTCTTAGCAGCCCCAG
  TCCTTTGGAATATAGCCCTCGGTCCCAGCCCCCCTCTCCAGGGCTCATTGCCTCCCCGGCACCTCCAGAC
  TTGGACCCAGAAGCTGTGCGTGATGCCCTCCGAGACTTCCTGCAAGAGCTGCGGAGCGCCCAGCGGGAGC
  GGGATGAACTTAAGGTCCAGACCAGCACCCTGAGCCAACAGCTGGTGGAGATGGAGGCAGAGAGAGACCA
  TGCAGCCTCAAGGGCCAAGCAGCTGCAGAAGGCAGTCGCTGAGAGCGAGGAAGCCTGGCGCAGTGCAGAC
  AGGCGGCTGAGCGGGGCCCAGGCAGAGCTGGCACTGCAGGAAGAGAGTGTGCGACGTAGCAAGCGGGAGT
  GCAGGGCCACGCTGGACCAGATGGCAGTGCTGGAGAGGAGCCTGCAGGCCACCGAGAGCGAGCTCCGAGC
  CAGCCAGGAGAAAGTCAGCAAGATGAAGGCCACCGAGGCGAAGCTGGAGAGCGACAAGCGGCGGCTGAAG
  GAGGTGCTGGATGCCTCTGAGAGCCGCTCCATCAAACTGGAGCTGCAGCGGCGCGCTCTTGAGGGCGAGC
  TGCAGCGCAGCCGCCTCGGCCTGGGGGACCGGGAGGCCCATGCGCAGGCCCTCCAGGATCGGGTGGACTC
  CCTGCAGAGACAGGTGGCAGACAGTGAGGTGAAGGCAGGGACCTTACAGCTGACAGTGGAGCGGCTCAGT
  GGGGCACTGGCCAAGGTGGAGGAGAGCGAGGGGAACCTGCGGAGCAAGGTGCAGAGCTTGACAGATGCCC
  TGACCCAGAGCAGCGCCAGCCTCAGCAGCACTCAGGACAAGAATCTGCACCTACAGAAGGCCTTGAGTAC
  CTGTGAACATGACCGCCAAGTGCTACAGGAACGGCTGGATGCAGCCAGACAGGCATTGTCTGAAGCGCGA
  AGACAAAGCAGCTCCCTGGGTGAACAGGTGCAGACTTTGAGGGGCGAGCTGGCCAGCCTGGAGCTGCAGC
  GGGGCGATGCTGAGGGCCAGCTGCAGCAGCTGCAGCAGGCGCTGCGACAGCGGCAGGAAGGGGAGGCCAT
  GGCTCTGCGCTCCGTCCAGAAGCTGCAGGAGGAGCGGCGCCTGTTGCAGGAGCGCCTGGGCAGTCTGCAG
  CGAGCCCTGGCCCAGCTGGAAGCTGAGAAACGTGATCTGGAGCGCTCAGCCCTGCAGTTCGACAAGGACC
  GTGTGGCCCTCAGGAAGACGCTGGATAAGGTGGAGCGGGAGAAGCTTCGAAGCCATGAGGACACCCTGCG
  CTTGAATGCAGAGAGGGGCCGCCTGGACCGCACACTCACAGGAGCTGAGCTGGACCTGGCCGAGGCCCAA
  CAGCAGATCCAACATTTGGAGGCACAGGTGGATGTAGCCCTGGAGGGGAACCACAATCCGGTCCAGCCAG
  AGGCGGGTGAGCAGCAACTGGAACTGCAACAGGAGGTTGAACGCCTGCGCAGTGCCCAGGTACAGACGGA
  GCGCACACTGGAGGCACGGGAGCGGGCCCACCGCCAGAGGGTGTCAGGGCTCGAGGAGCAGGTATCCACA
  CTGAAGGCACAGCTACACCAGGAGCTTCGCAGGAGCTCGGCATCCGTCTCCCTACCCCCTGGCACCCCTG
  AGAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001145958
• Insert Size: 5538 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145958.1, NP_001139430.1
• RefSeq Size: 6282 bp
• RefSeq ORF: 5538 bp
• Locus ID: 230872
• UniProt ID: Q8CJ40
• Cytogenetics: 4 D3
• Gene Summary: Major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells which originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus (PubMed:12427867). Furthermore, is required for the correct positioning of the cilium basal body relative to the cell nucleus, to allow for ciliogenesis (By similarity). Contributes to centrosome cohesion before mitosis (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus and lacks an alternate internal segment compared to isoform 1.