Cacna1s (NM_014193) Mouse Untagged Clone

CAT#: MC224959

Cacna1s (untagged) - Mouse calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cacna1s
• Synonyms: AW493108; Cav1.1; Cchl1a3; fmd; mdg; sj
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224959 representing NM_014193
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGCCCTCACCCCAGGACGAGGGCCTGAGGAAGAAGCAGCCGAAGAAACCTGTTCCTGAGATTC
  TGCCGAGGCCCCCGCGGGCCTTGTTCTGCCTGACTCTACAGAACCCCCTGAGGAAGGCCTGCATCAGCAT
  CGTGGAATGGAAACCCTTCGAGACCATCATCTTGCTCACCATCTTTGCCAACTGTGTGGCCCTGGCAGTG
  TACCTGCCCATGCCAGAGGATGACAACAACACTCTGAACCTCGGCCTGGAGAAACTGGAGTACTTCTTCC
  TCATTGTCTTCTCCATTGAAGCCGCCATGAAGATCATAGCCTACGGCTTCCTATTCCATCAGGATGCATA
  CCTGCGCAGTGGCTGGAACGTTCTGGACTTCATCATTGTCTTTCTGGGGGTCTTCACTGTGATTCTGGAA
  CAAGTTAACATCATTCAAACCAACACGGCCCCCATGAGCAGCAAAGGAGCCGGCTTGGATGTGAAGGCCC
  TTCGAGCATTCCGCGTGCTCAGACCCCTGCGGCTGGTGTCGGGGGTGCCCAGTCTTCAGGTGGTCCTCAA
  CTCCATCTTCAAGGCCATGCTCCCCCTGTTCCACATTGCCCTGCTCGTCCTCTTCATGGTCATCATCTAC
  GCCATCATTGGGCTGGAGCTCTTCAAAGGCAAGATGCACAAGACTTGCTACTTCATTGGGACAGACATTG
  TAGCCACAGTTGAGAATGAGAAACCATCACCCTGTGCTAGAACTGGCTCTGGGCGTCCCTGCACCATCAA
  TGGCAGCGAGTGCCGAGGAGGTTGGCCAGGACCCAACCACGGCATCACCCACTTCGACAACTTTGGCTTC
  TCTATGCTCACTGTGTATCAATGCATCTCCATGGAGGGGTGGACTGATGTCCTGTACTGGGTCAACGATG
  CCATCGGGAATGAGTGGCCCTGGATCTACTTTGTCACCCTCATTCTGCTGGGCTCCTTCTTCATCCTCAA
  CCTGGTGCTGGGTGTCCTGAGTGGAGAATTCACCAAAGAGCGAGAGAAGGCCAAGTCCAGGGGAACCTTC
  CAAAAGCTCCGAGAGAAACAGCAGCTGGAGGAGGACCTCCGTGGTTACATGAGCTGGATCACACAGGGCG
  AGGTCATGGACGTGGACGACTTGAGAGAAGGCAAGCTGTCTTTGGATGAAGGGGGATCAGACACAGAGAG
  CTTGTATGAAATCGAAGGCTTGAACAAAATCATCCAGTTCATTCGGCACTGGCGACAGTGGAATCGCGTC
  TTCCGCTGGAAGTGCCATGACCTAGTGAAATCCAAGGTCTTCTATTGGCTGGTGATCCTGATCGTTGCCC
  TAAACACCCTGTCCATTGCCTCGGAGCACCACAACCAGCCACTCTGGTTGACCCATTTGCAAGATGTTGC
  CAACCGCGTGTTGCTGACCCTCTTCACCATCGAAATGCTCATGAAGATGTACGGGCTGGGCCTGCGCCAG
  TACTTCATGTCCATCTTCAACCGCTTCGACTGCTTCGTGGTGTGCAGCGGCATCCTGGAGATCCTGCTGG
  TGGAGTCGGGAGCCATGTCGCCCCTGGGCATCTCTGTGCTCCGCTGTATCCGCCTCCTGAGGCTCTTCAA
  GATCACCAAGTACTGGACATCGCTGAGCAACCTGGTGGCCTCACTGCTCAACTCCATCCGCTCCATTGCT
  TCCCTCTTGCTGCTGCTCTTTCTCTTCATCATCATCTTTGCCCTGCTGGGCATGCAGCTCTTTGGAGGCA
  GATATGACTTTGAAGACACAGAAGTACGACGAAGCAACTTTGACAACTTCCCCCAGGCCCTCATCAGTGT
  CTTCCAGGTCCTGACGGGTGAAGACTGGAACTCTGTGATGTACAATGGGATCATGGCTTACGGAGGTCCG
  ACCTACCCTGGTGTGCTGGTGTGCATTTATTTCATCATCCTTTTCGTCTGTGGCAACTACATCCTTCTCA
  ACGTCTTCCTGGCTATTGCTGTGGACAACCTGGCAGAGGCAGAGAGTCTGACATCTGCCCAAAAGGCCAA
  GGCTGAGGAGAGAAAGCGCAGGAAGATGTCGAAAGGGCTCCCAGACAAGTCAGAAGAGGAGAGGGCCACG
  GTGACCAAGAAACTGGAGCAGAAGTCCAAGGGTGAAGGCATCCCCACCACCGCCAAGCTGAAAATCGATG
  AGTTTGAATCAAACGTTAATGAGGTGAAAGACCCCTACCCTTCGGCTGACTTCCCAGGGGACGATGAGGA
  AGATGAGCCCGAGATCCCAGTGAGCCCCCGACCACGCCCTCTGGCCGAGCTGCAGCTGAAAGAGAAAGCA
  GTGCCCATCCCGGAGGCTAGCTCCTTCTTCATCTTCAGTCCCACCAACAAGATCCGTGTCCTGTGTCACC
  GCATTGTCAATGCCACCTGGTTCACCAACTTCATCCTGCTCTTCATCCTGCTCAGCAGTGCCGCCCTGGC
  CGCTGAGGATCCCATCCGGGCCGACTCCATGAGGAATCAGATCCTCGAGTATTTTGATTATGTGTTCACT
  GCTGTTTTCACGGTGGAGATTGTCCTTAAGATGACCACCTATGGAGCCTTCCTGCACAAAGGCTCCTTCT
  GCCGCAACTACTTCAACATCCTGGACCTGCTGGTGGTGGCTGTGTCCCTCATCTCCATGGGCCTCGAGTC
  CAGCGCCATCTCCGTGGTGAAGATCTTAAGGGTTCTGAGAGTGCTGAGACCACTCCGGGCCATAAACAGA
  GCCAAAGGGTTGAAGCACGTGGTGCAGTGCGTGTTTGTTGCCATTCGCACCATCGGGAACATCGTGCTGG
  TCACCACGCTCCTGCAGTTCATGTTTGCGTGCATTGGAGTGCAGCTTTTCAAGGGAAAGTTCTACAGCTG
  CAATGACTTATCCAAGATGACTGAAGAGGAATGCAGGGGCTACTACTACATATACAAGGATGGAGACCCC
  ACTCAGATAGAGCTGCGTCCCCGCCAGTGGATACACAATGACTTCCACTTTGACAATGTACTCTCCGCTA
  TGATGTCACTCTTCACGGTCTCCACCTTCGAGGGTTGGCCTCAGCTACTGTACAAGGCCATAGACTCAAA
  TGAGGAGGACACCGGCCCTGTCTACAACAACCGAGTGGAGATGGCCATCTTCTTCATTATCTACATCATC
  CTCATTGCCTTCTTCATGATGAACATCTTTGTGGGCTTTGTCATCGTTACCTTCCAGGAACAGGGAGAGA
  CTGAGTATAAGAACTGTGAGCTCGACAAGAACCAGCGCCAATGCGTACAGTATGCCCTGAAGGCCCGCCC
  ACTGAGGTGTTACATCCCCAAAAACCCATACCAGTATCAGGTGTGGTATGTCGTCACTTCCTCCTACTTT
  GAATACCTGATGTTTGCTCTCATCATGCTCAATACTATCTGCCTGGGCATGCAGCATTACAACCAGTCGG
  AACAGATGAACCACATCTCAGACATTCTCAACGTGGCTTTCACCATCATCTTCACCCTGGAGATGGTCCT
  CAAGCTCATAGCTTTCAAGCCCAGGGGCTATTTTGGAGATCCTTGGAATGTGTTTGACTTCCTAATCGTC
  ATCGGCAGCATCATTGACGTCATCCTGAGCGAGATCGATGACCCAGATGAGAGCGCCCGTATCTCCAGTG
  CCTTCTTCCGCCTGTTCCGGGTCATGAGACTGGTCAAGCTGCTGAACCGGGCGGAGGGTGTGCGCACCCT
  GCTGTGGACATTCATCAAGTCCTTCCAGGCCCTGCCGTACGTGGCTTTGCTGATCGTGATGCTCTTCTTC
  ATCTACGCGGTCATCGGCATGCAGATGTTCGGGAAGATCGCCATGGTGGACGGGACGCAGATAAACCGGA
  ACAACAACTTCCAGACTTTCCCACAGGCCGTGCTGCTGCTCTTCAGGTGTGCCACAGGGGAAGCCTGGCA
  AGAGATCCTGCTGGCCTGCAGCTATGGGAAGCTGTGTGACCCGGAGTCTGACTACGCACCCGGGGAGGAG
  CATACATGCGGCACCAACTTCGCCTACTACTACTTCATCAGTTTCTACATGCTCTGCGCCTTCCTGATCA
  TCAACCTCTTTGTGGCTGTCATCATGGACAACTTTGATTACCTCACAAGGGACTGGTCCATTCTGGGCCC
  TCACCACCTGGACGAGTTCAAGGCCATTTGGGCTGAGTACGACCCGGAGGCTAAGGGCAGGATCAAGCAC
  CTGGATGTGGTGACCCTGCTGAGGAGGATTCAGCCCCCTCTGGGCTTTGGGAAGTTCTGCCCACACAGGG
  TAGCATGTAAGAGGCTGGTGGGCATGAACATGCCCCTGAACAGCGATGGCACAGTCACCTTTAATGCCAC
  ACTATTTGCCCTGGTGCGCACAGCCCTCAAGATCAAGACAGAAGGCAACTTTGAACAAGCTAACGAGGAG
  CTGAGAGCCATCATCAAGAAGATCTGGAAGAGAACCAGCATGAAGCTCTTGGACCAAGTCATCCCTCCAA
  TAGGAGATGATGAGGTGACAGTAGGGAAATTCTATGCCACATTCCTCATCCAGGAGCACTTCCGGAAGTT
  CATGAAGCGCCAGGAGGAATATTATGGGTATCGACCCAAAAAGGACACAGTTCAGATACAGGCTGGATTA
  CGGACCATAGAGGAAGAGGCAGCTCCTGAGATCCACCGTGCCATCTCTGGAGACCTGACAGCAGAGGAGG
  AACTGGAGAGGGCCATGGTAGAGGCTGCCATGGAGGAGGGGATATTCCGGAGGACGGGAGGCCTGTTTGG
  TCAAGTGGACAACTTCCTGGAAAGGACCAACTCTCTGCCCCCTGTCATGGCCAACCAAAGACCCCTCCAA
  TTTGCTGAAATAGAGATGGAAGAGCTGGAGTCACCAGTCTTCTTGGAGGACTTCCCTCAGAACCCAGGCA
  CCCACCCTCTTGCTCGTGCCAATACCAACAACGCCAATGCCAATGTTGCCTATGGCAACAGCAGCCATAG
  AAACAACCCAGTGTTTTCCAGCATCTGCTATGAACGGGAGTTCTTGGGAGAGGCGGACATGCCTGTTACC
  AGAGAAGGACCCCTCAGTCAGCCCTGCAGGGCCTCAGGACCTCACAGCAGATCTCATGTGGACAAACTGA
  AAAGACCGATGACCCAGAGAGGAATGCCTGAAGGCCAGGTGCCTCCTTCCCCGTGCCAGCTCTCCCAGGC
  AGAGCATCCTGTGCAGAAAGAGGGAAAGGGCCCCACGTCACGGTTTCTGGAAACACCCAACAGCAGGAAC
  TTTGAGGAGCACGTTCCCAGGAATTCGGCTCACAGGTGCACGGCTCCAGCTACTGCCATGCTGATCCAGG
  AGGCTCTGGTTCGAGGAGGACTAGACTCCTTAGCAGCAGATGCTAACTTCGTCATGGCAACAGGCCAGGC
  CCTGGCAGATGCCTGCCAGATGGAACCAGAGGAAGTCGAGGTGGCAGCAACAGAGCTACTGAAGCAAGAA
  TCCCCAGAGGGCGGGGCCGTGCCCTGGGAGCCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_014193
• Insert Size: 5496 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014193.2, NP_055008.2
• RefSeq Size: 6188 bp
• RefSeq ORF: 5496 bp
• Locus ID: 12292
• Cytogenetics: 1 59.55 cM