Itgb4 (NM_133663) Mouse Untagged Clone

CAT#: MC224908

Itgb4 (untagged) - Mouse integrin beta 4 (Itgb4), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Itgb4
• Synonyms: AA407042; C230078O20; CD104
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224908 representing NM_133663
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGGCCCTGTTGCAGCCCATGGGTGAAGCTGCTGCTGCTGGCAGCAATGCTGAGTGCCAGCCTCC
  CTGGAGACCTGGCCAACCGCTGCAAGAAGGCTCAGGTGAAGAGCTGTACCGAGTGCATCCGGGTGGACAA
  GAGCTGTGCCTACTGCACAGACGAGCTGTTCAAGGAGAGGCGCTGCAACACCCAGGCGGAGCTTCTGGCT
  GCAGGCTGCAGGGGAGAGAGCATCCTGGTCATGGAGAGCAGCCTTGAAATCACAGAGAACACCCAGATCG
  ACACCAGCCTGCACCGCAGCCAGGTATCTCCCCAAGGCCTGCAAGTCCGGCTGCGGCCGGGTGAGGAGCG
  CAGCTTTGTGTTCCAGGTGTTTGAGCCCCTGGAGAGCCCCGTGGATCTGTATATCCTCATGGACTTCTCC
  AACTCCATGTCTGACGATCTGGACAACCTCAAGCAGATGGGGCAGAACCTGGCCAAGATCCTGCGCCAGC
  TCACCAGCGACTACACCATTGGATTTGGAAAGTTTGTGGACAAAGTCAGCGTCCCACAGACAGACATGAG
  GCCCGAGAAACTGAAGGAGCCCTGGCCCAACAGTGATCCCCCGTTCTCCTTCAAGAACGTTATCAGCTTA
  ACGGAGAATGTGGAAGAATTCTGGAACAAACTGCAAGGAGAACGCATCTCAGGCAACCTGGACGCTCCTG
  AAGGGGGCTTTGATGCCATCCTGCAGACAGCTGTGTGCACAAGGGACATTGGCTGGAGGGCTGACAGCAC
  CCACCTGCTGGTGTTCTCCACCGAGTCTGCCTTCCACTACGAGGCTGATGGTGCCAACGTGCTGGCCGGC
  ATCATGAACCGCAATGATGAGAAATGCCACCTGGACGCCTCGGGCGCCTACACCCAATACAAGACACAGG
  ACTACCCATCAGTGCCCACGCTGGTTCGCCTGCTTGCCAAGCATAACATCATCCCCATCTTTGCTGTCAC
  CAACTACTCTTACAGCTACTATGAGAAGCTCCATAAGTATTTCCCCGTCTCCTCTCTGGGCGTCCTGCAG
  GAGGATTCATCCAACATCGTGGAGCTGCTGGAGGAGGCCTTCTATCGAATTCGCTCCAACCTGGACATCC
  GGGCTCTGGACAGCCCCAGAGGCCTGAGAACAGAGGTCACCTCCGATACTCTCCAGAAGACGGAGACTGG
  GTCCTTTCACATCAAGCGGGGGGAAGTGGGCACATACAATGTGCATCTCCGGGCAGTGGAGGACATAGAT
  GGGACACATGTGTGCCAGCTGGCTAAAGAAGACCAAGGGGGCAACATCCACCTGAAACCCTCCTTCTCTG
  ATGGCCTCCGGATGGACGCGAGTGTGATCTGTGACGTGTGCCCCTGTGAGCTGCAAAAGGAAGTTCGATC
  AGCTCGCTGTCACTTCAGAGGAGACTTCATGTGTGGACACTGTGTGTGCAATGAGGGCTGGAGTGGCAAA
  ACCTGCAACTGCTCCACCGGCTCTCTGAGTGACACACAGCCCTGCCTGCGTGAGGGTGAGGACAAACCGT
  GCTCGGGCCACGGCGAGTGCCAGTGCGGACGCTGTGTGTGCTATGGTGAAGGCCGCTACGAGGGTCACTT
  CTGCGAGTATGACAACTTCCAGTGTCCCCGGACCTCTGGATTCCTGTGCAATGACCGGGGTCGCTGTTCT
  ATGGGAGAGTGTGTGTGTGAGCCTGGTTGGACAGGCCGCAGCTGTGACTGTCCCCTCAGCAATGCCACCT
  GCATCGATAGCAACGGGGGCATCTGCAACGGCCGAGGCTACTGTGAGTGTGGCCGTTGTCACTGCAACCA
  GCAGTCGCTCTACACGGACACCACCTGTGAGATCAACTACTCTGCGATACGCCTGGGTCTCTGTGAGGAT
  CTCCGCTCCTGCGTACAGTGCCAGGCCTGGGGCACCGGGGAGAAGAAAGGGCGCGCGTGTGACGATTGCC
  CCTTTAAAGTCAAGATGGTAGACGAGCTTAAGAAAGAAGAGGTGGTGGAGTACTGCTCCTTCCGGGATGA
  GGATGACGACTGCACTTACAGCTACAACGTGGAGGGCGATGGCAGCCCTGGGCCCAACAGCACAGTCCTG
  GTCCACAAAAAGAAAGACTGCCCTCCCGGCTCCTTCTGGTGGCTCATCCCCCTGCTCATCTTCCTCCTGT
  TGCTCCTGGCGTTGCTTCTGCTGCTCTGCTGGAAATACTGTGCCTGCTGCAAAGCCTGCCTGGGGCTTCT
  TCCTTGCTGCAACCGAGGTCACATGGTGGGCTTTAAGGAAGATCACTATATGCTTCGGGAGAACCTGATG
  GCCTCTGACCACCTGGACACGCCCATGCTACGAAGCGGGAACCTCAAGGGACGAGACACAGTCCGCTGGA
  AGATCACCAACAATGTGCAGCGCCCTGGCTTTGCCACCCATGCCGCCAGCACCAGCCCCACGGAGCTCGT
  ACCCTACGGGCTGTCCCTGCGCCTTGGCCGCCTCTGCACTGAGAACCTTATGAAGCCGGGCACCCGAGAG
  TGTGACCAGCTACGCCAGGAGGTGGAGGAAAATCTGAATGAGGTGTATAGACAGGTCAGCGGCGCACACA
  AGCTCCAGCAGACGAAGTTCCGACAGCAGCCCAACACCGGGAAAAAGCAAGACCACACCATTGTGGACAC
  AGTGTTGCTGGCGCCCCGCTCCGCCAAGCAGATGCTGCTGAAGCTGACAGAGAAGCAGGTGGAGCAGGGG
  TCCTTCCATGAACTGAAGGTGGCCCCTGGCTACTACACTGTCACGGCAGAGCAGGATGCCCGGGGCATGG
  TGGAGTTCCAGGAGGGCGTGGAGCTGGTGGATGTGCGAGTGCCCCTCTTCATCCGGCCTGAGGATGATGA
  TGAGAAGCAGCTGCTGGTGGAGGCCATTGATGTCCCTGTGGGCACTGCCACCCTTGGTCGCCGTCTGGTA
  AACATCACCATTATCAAGGAACAAGCTAGTGGGGTAGTGTCCTTCGAGCAGCCTGAATACTCGGTGAGTC
  GTGGAGACCAGGTGGCCCGCATCCCTGTCATCCGGCACATCCTGGACAATGGCAAGTCCCAGGTCTCCTA
  TAGCACACAGGATAATACAGCACACGGACACCGGGATTATGTCCCTGTGGAGGGAGAGCTGCTGTTCCAT
  CCTGGGGAGACCTGGAAGGAGTTGCAGGTGAAGCTACTGGAGCTGCAGGAGGTCGACTCCCTCCTGCGTG
  GCCGCCAGGTCCGCCGCTTCCAAGTCCAACTCAGCAACCCCAAGTTCGGAGCCCGCCTGGGCCAGCCCAG
  CACAACCACCGTTATTCTCGGTGAGCACGATGAAACGGACAGGAGTCTCATAAATCAAACACTTTCATCG
  CCTCCGCCACCCCATGGAGACCTGGGCGCCCCACAGAACCCCAATGCCAAGGCTGCCGGGTCCAGGAAGA
  TCCATTTCAACTGGCTGCCCCCTCCTGGCAAGCCAATGGGGTACAGGGTGAAGTACTGGATCCAGGGCGA
  CTCTGAGTCTGAAGCCCACCTTCTAGACAGCAAGGTGCCCTCAGTGGAACTCACCAACCTGTATCCCTAT
  TGCGACTACGAAATGAAGGTGTGTGCCTATGGGGCACAGGGTGAGGGGCCCTATAGCTCACTGGTGTCCT
  GCCGCACCCACCAGGAAGTACCCAGCGAGCCAGGGAGGCTGGCTTTCAATGTAGTCTCTTCTACGGTGAC
  GCAGCTGAGCTGGGCAGAGCCAGCTGAGACCAATGGCGAGATCACAGCCTACGAGGTCTGCTATGGACTG
  GTCAATGAGGACAACCGACCCATTGGACCTATGAAGAAGGTGCTCGTGGACAACCCCAAGAACCGGATGC
  TGCTCATTGAGAATCTGCGAGAATCCCAGCCATACCGATACACGGTTAAGGCGCGCAATGGGGCAGGATG
  GGGACCCGAGAGAGAGGCTATCATCAACCTGGCTACACAGCCCAAGCGGCCCATGTCCATCCCTATCATC
  CCAGACATCCCCATAGTGGACGCCCAGGGTGGAGAAGACTACGAAAACTTCCTTATGTACAGTGATGACG
  TCCTGCGGTCCCCAGCCAGCAGCCAGAGGCCCAGCGTTTCTGATGACACTGAGCACCTGGTGAATGGCCG
  GATGGACTTTGCCTATCCAGGCAGCGCCAACTCCCTGCACAGAATGACTGCAGCCAATGTGGCCTATGGC
  ACGCATCTGAGCCCACACCTGTCCCACCGAGTGCTGAGCACGTCCTCCACCCTTACCCGGGACTACCACT
  CTCTGACACGCACAGAGCACTCCCACTCAGGCACACTTCCCAGGGACTACTCCACCCTCACTTCCCTTTC
  CTCCCAAGATTCCCGTGGGGCTGTGGGTGTGCCTGACACACCCACTCGGCTGGTGTTCTCTGCCCTGGGG
  CCCACGTCTTTGAAGGTGAGCTGGCAGGAGCCACAGTGTGATCGGATGCTGCTGGGCTACAGTGTGGAAT
  ACCAGCTACTAAACGGCGGCGAGATGCATCGGCTCAACATCCCTAACCCTGGCCAAACCTCGGTGGTGGT
  AGAGGATCTCCTGCCTAACCACTCCTATGTGTTCCGGGTACGGGCCCAGAGCCAGGAGGGCTGGGGCCGA
  GAGCGAGAGGGTGTCATCACCATCGAGTCCCAGGTGCACCCGCAGAGCCCTCTCTGCCCCCTGCCAGGCT
  CAGCCTTCACTCTGAGCACCCCCAGCGCCCCGGGACCGCTGGTGTTCACTGCCCTAAGCCCAGACTCCCT
  GCAGCTCAGCTGGGAGCGGCCGAGGAGGCCCAATGGAGATATCCTTGGCTACCTGGTGACCTGTGAGATG
  GCCCAAGGAGGAGCACCAGCCAGGACCTTCCGGGTGGACGGAGACAACCCTGAGAGCCGGTTGACTGTAC
  CTGGCCTCAGTGAGAACGTTCCTTACAAGTTCAAGGTTCAGGCCAGGACGACCGAGGGCTTCGGGCCAGA
  GCGTGAGGGTATCATCACCATCGAGTCTCAGGTTGGAGGCCCCTTCCCACAGCTGGGCAGCCATTCTGGG
  CTCTTCCAGAACCCAGTGCAAAGCGAGTTCAGCAGCGTGACCAGCACGCACAGCACCACGACTGAGCCCT
  TCCTCATGGATGGTCTAACCCTGGGGACCCAGCGCCTGGAAGCAGGAGGCTCCCTCACCCGGCATGTGAC
  CCAGGAATTCGTGACCCGGACCTTAACGGCCAGTGGCTCTCTCAGCACTCATATGGACCAACAGTTCTTC
  CAAACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133663
• Insert Size: 5259 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133663.2, NP_598424.2
• RefSeq Size: 6010 bp
• RefSeq ORF: 5259 bp
• Locus ID: 192897
• Cytogenetics: 11 80.91 cM
• Gene Summary: Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses a different splice site and lacks an alternate in-frame exon in the 3' coding region, as compared to variant 1. The resulting protein (isoform 2) thus has two differences with a shorter C-terminus when compared to isoform 1.