Prex1 (NM_177782) Mouse Untagged Clone

CAT#: MC224790

Prex1 (untagged) - Mouse phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1 (Prex1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prex1
• Synonyms: G630042G04; P-REX1; Setd6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224790 representing NM_177782
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCACCCGGCAGCGGTGGTGGTGACGGCGGCGGGGACCCAGGCGGGGACGGTGCGCATCCCGATG
  CTCGCGGCCCCGTCTCCGGGCCGTGCGCGGCCGCCCGCGACTCGGAGCGCCAGCTGCGCCTCCGTCTGTG
  CGTACTCAACGAGATCCTGGGCACCGAGCGCGACTACGTGGGCACCTTGCGTTTCCTGCAGTCGGCCTTC
  CTACAGCGGATCCGGCAGAATGTGGCCGACTCTGTGGAGAAGGGCCTCACAGAAGAGAATGTCAAGGTCC
  TCTTCTCCAACATTGAGGACATCCTGGAAGTCCATAAGGATTTTCTGGCTGCCCTGGAGTACTGCTTACA
  CCCCGAGCCTCAGTCTCAGCACGAACTTGGGAATGTTTTCTTGAAATTTAAGGACAAGTTCTGTGTGTAT
  GAGGAGTACTGCAGCAACCACGAGAAGGCCCTGCGCCTGTTGGTGGAGCTCAACAAGGTCCCTGCTGTGC
  GTGCCTTCCTCCTGAGCTGCATGCTTCTGGGGGGCCGGAAGACCACTGACATTCCTCTGGAAGGCTACCT
  GCTGTCCCCGATCCAGAGAATCTGCAAGTACCCGCTCCTGCTCAAGGAGTTGGCCAAGAGGACCCCCGGC
  AAGCACCCTGACCACACCGCGGTACAGAGTGCCCTGCAGGCCATGAAGACTGTCTGCTCCAACATCAATG
  AGACTAAGAGGCAGATGGAGAAGCTGGAGGCCCTGGAGCAGCTGCAGTCTCACATTGAAGGCTGGGAGGG
  CTCCAACCTCACAGACATCTGCACCGAGCTCCTCCTTCAGGGCAACCTGCTGAAGATCTCTGCGGGCAAC
  ATCCAAGAGAGAGCCTTCTTCCTCTTTGACAACCTGCTTGTCTACTGCAAGCGGAAGTCTAGGGTCACTG
  GGAGCAAGAAATCTACCAAAAGGACCAAGTCCATCAACGGCTCCCTGTACATCTTTAGGGGTCGGATCAA
  CACTGAGGTCATGGAAGTGGAGAATGTGGAGGATGGGACAGCCGATTACCACAGCAATGGCTACACTGTC
  ACCAATGGCTGGAAGATCCACAACACCGCCAAAAACAAGTGGTTCGTGTGTATGGCCAAGACGGCGGAGG
  AGAAACAGAAGTGGCTGGATGCCCTGATCCGAGAGCGGGAGCAGCGAGAGAGCCTGAAGCTGGGCATGGA
  GCGTGACGCGTACGTCATGATTGCGGAGAAGGGGGAGAAGCTGTATCACATGATGATGAGCAAGAAGGTG
  AACTTGATCAAGGACCGCCGGCGGAAGCTGAGCACCGTCCCCAAGTGCTTCCTTGGCAATGAGTTTGTTG
  CCTGGCTCCTGGAAATTGGTGAGATCAGCAAGACGGAAGAAGGCGTCAACTTGGGCCAAGCCCTGCTGGA
  GAATGGTATCATCCACCATGTCTCTGACAAGCACCAGTTCAAGAACGAGCAGGTGATGTACCGCTTCCGC
  TACGACGACGGCACTTACAAAGCGCGCAGCGAGCTGGAGGATATCATGTCCAAGGGCGTGAGACTCTACT
  GCCGGCTGCACAGCCTGTACGCCCCTGTGATCAAGGACCGTGATTACCACCTGAAGACCTACAAGTCCGT
  GGTCCCCGGGAGCAAGCTGGTAGACTGGCTACTGGCACAGGGGGACTGCCAGACACGGGAGGAGGCCGTG
  GCGCTTGGTGTGGGCCTGTGCAACAACGGTTTTATGCACCATGTTCTGGAGAAGAGCGAATTCAAGGATG
  AGTCCCAGTACTTCCGCTTCCATGCTGATGAGGAGATGGAGGGGACCAGCAGCAAGAACAAACAGCTTCG
  CAATGACTTCAAGCTGGTGGAGAACATCCTGGCAAAGCGACTGCTGATCCCGCCACAGGAGGACGACTAT
  GGCTTCGACCTTGAGGAGAAGAACAAGGCTGTGGTAGTGAAGTCTGTGCAGAGGGGCTCGCTGGCTGAGA
  TGGCTGGCCTGCAGGCTGGGAGGAAGATTTACTCCATCAACGAGGACCTGGTGTTCCTGCGTCCCTTCTC
  TGAGGTGGAGACCATCCTCAACCAGTTCTTCTGCTCCCGCCGCCCGCTCCGCCTCCTGGTGGCCACCAAG
  GCCAAAGAGACCATCAAAGTCCCTGACCACCCAGAGGCCCTGTCCTTCCAGATTCGAGGAACAGCCCCGC
  CGTGTGTCTTTGCTGTGGGCAGAGGCTCTGAGGCTGTGGCTGCCGGGCTCTGCGCGGGCCAATGCATCCT
  GAAAGTCAACGGCACCAGCGTGGCGAATGATGGCGCCCTGGAGGTTCTCGAGCACTTCCAGGCTTTCCGC
  AACCACAGGGAGGAGGCCCTGGGTCTTTACCAGTGGGTCTACCACAGCCACGAGGATGCCCAGCTGGCAC
  GAGCCAGCCAGGGTGCCCCTGATGAGGACCCCCAGGAAGATGACCAGCCTGACTCAGCCCTGCCCCTGCT
  GTCCCTGGGTCCCCAGCTGAGTCTGCATGAGGACAGCGCTGTGGTCAGCCTGACGCTGGACAATGTTCAC
  TTGGAGCACGGTGTGGTTTACGAATACATGAGCACTGCAGGAGCCAAGTGTCACGTGCTGGAGAAGATCG
  TGGAGCCCCGAGGCTGTTTCCGCCTTGCTGCCAAGATCCTTGAGGCCTTTGCTGTGGACGACAGCATCTT
  CGTGCAGAACTGTGGGCGGCTCATGGCCATGAGCAGTGCCATTGTCACCATGTCCCACTACGAGTTCCAT
  AACATCTGTGACACCAAGCTGGAGAGCATCGGCCAGAGGATCGCCTGCTACCAGGAGTTTGCAGCCCAGC
  TGAAGAGTAGGGTCAGCCCCCCCTTCAAGCAGGCATCCCTGGAGCCCCACCCGCTGTGTGGCCTTGACTT
  CTGCCCCACCAACTGTCACGTCAACCTCATGGAAGTGTCCTACCCCAAGACCACACCTTCGGTGGGCAGG
  TCCTTCAGCATCCGCTTCGGACGCAAACCCTCCCTCATCGGCCTTGACCCAGAACAAGGCCTGAACCCCA
  TGGCCTATACCCAGCACTGTATCACCACCATGGCTGCCCCGTCCTGGAAGTGCTCACCTGCTGTAGACGA
  GGACTCCCAAGGCCAGGGTCTCAATGACAGCAGCTATGGGTCAGCCAGTGGTGCCCCGAGCCAGCAAGAC
  CGCGGCCTGAGCTTTCTGCTCAAACAGGAAGACCGTGAGATCCAGGACGCATACCTGCAGCTCTTCACTA
  AGCTGGACGTGGCCCTCAAGGAGATGAAGCAGTATGTCACCCAGATTAACAGGCTGCTGTCAACCATCAC
  AGAGCCCACATCAGCTGCCCCTGCGCCCTGTGACCCATCCTTGGTGGAGGAGACCTCATCTTCCCCACCG
  GTCAGTGAGGAGAGTGAAGTGGACCGGACTGACCACAGCGGCATCAAGAAAGTGTGCTTCAAGGTGTCGG
  AGGACGAGCAGGAAGACTCGGGCCACGACACCATGAGCTACCGCGACTCCTACAGTGAGTGTAACAGCAA
  TCGGGACTCTGTCCTGTCCTACACCAGCGTGAGAAGCAACAGTTCGTACCTGGGTAGTGACGAGATGGGC
  TCAGGGGATGAGCTGCCCTGTGACATGCGCATCCCGTCCGACAAGCAGGACAAACTTCACGGCTGCCTGG
  AACATCTCTTTAACCAGGTCGATTCCATCCATGCCCTGCTCAAGGGGCCTGTGATGAGCAGGGCATTTGA
  GGAGACCAGGCATTTCCCCATGAAGCACAGCTGGCAAGAGTTCAAACAGAAAGAGGAATGCACGGTCCGA
  GGGCGGAACTTGATTCAGATCAGTATCCAGGAGGACCCTTGGAACCTGCCCAGCTCCATCAGGACCCTGG
  TGGACAACATCCAGCAATACGTGGAAGATGGAAAGAACCAGCTGCTTCTGGCTCTGCTCAAGTGCACAGA
  CACGGAGCTGCAGCTGCGCAGGGATGCTGTCTTCTGTCAGGCTCTGGTGGCCGCCGTGTGCACTTTCTCG
  GAGCAGCTGCTGGCCGCCCTTGACTACCGCTACAACAACAACGGCGAGTATGAAGAGAGCAGCAGAGATG
  CCAGCCGCAAGTGGCTGGAGCAGGTGGCGGCTACAGGTGTCCTGCTGCACTGGCAGTCTCTGCTGGCACC
  AGCCTCCGTGAAGGAGGAACGGACCATGCTGGAGGACATCTGGGTGACGCTGTCGGAGCTGGACAATGTC
  ACCTTCTCCTTTAAGCAGCTGGATGAGAATTCTGTGGCCAACACCAATGTCTTCTACCACATCGAGGGCA
  GTAGGCAGGCACTGAAGGTAGTCTTCTACCTCGATGGCTTCCACTTCTCCAGGCTGCCCTCTCGCCTAGA
  GGGTGGGGCCAGCCTCAGGCTGCACACGGTGCTCTTCACAAAAGCACTGGAGAGCGTGGAAGGGCCCCCG
  CCCCCTGGAAACCAGGCAGCAGAAGAGCTGCAGCAGGAGATCAACGCACAGTCCCTGGAGAAGGTGCAGC
  AGTACTATAGAAAGCTCAGGGCGTTCTACCTGGAGCGGTCCAACCTGCCCACGGATGCTGGCGCCACAGC
  TGTGAAGATTGACCAGCTGATTCGTCCCATCAATGCCCTGGATGAGCTCTACCGCCTCATGAAGACCTTC
  GTGCACCCCAAAGCGGGTGCTGCTGGGAGCCTGGGTGCTGGTCTCATCCCCGTCTCCTCCGAGCTCTGCT
  ATCGCCTGGGGGCGTGTCAGATCACCATGTGTGGCACCGGCATGCAGCGGAGCACCCTGAGCGTGTCCTT
  GGAACAAGCAGCCATCTTGGCACGGAGTCACGGCCTGTTGCCCAAGTGTGTCATGCAGGCCACAGACATC
  ATGCGCAAGCAGGGCCCCCGAGTGGAGATTCTGGCCAAAAACCTCCGCATCAAGGACCCCATGCCCCAAG
  GTGCACCACGCCTCTACCAGCTCTGCCAGCCTCCGGTGGATGGAGACCTCTGA
  
  CAAGCTTAACTAGCTAGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATC
  TGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAAAC
• Restriction Sites: SgfI-HindIII
• ACCN: NM_177782
• Insert Size: 4953 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177782.3, NP_808450.2
• RefSeq Size: 6530 bp
• RefSeq ORF: 4953 bp
• Locus ID: 277360
• UniProt ID: Q69ZK0
• Cytogenetics: 2 H3
• Gene Summary: Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils (By similarity).[UniProtKB/Swiss-Prot Function]