Col11a2 (NM_009926) Mouse Untagged Clone

CAT#: MC224786

Col11a2 (untagged) - Mouse collagen, type XI, alpha 2 (Col11a2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Col11a2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224786 representing NM_009926
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGTGCAGCCGCTGCCACCGCCTCCTTCTGTTCCTACCTCTGGTGCTGGGTCTGAGCGCTGCCC
  CGGGATGGGCAGGTGCTCCCTCTGTGGATGTGCTTCGTGCCCTGAGGTTCCCCTCCCTTCCCGATGGTGT
  TCGGAGATCAAAAGGGGTCTGTCCGGGTGATGTGGCTTACCGTGTGGCACGGCCTGCCCAGCTCAGCGCA
  CCCACGCGCCAGCTCTTCCCAGGAGGCTTTCCCAAAGACTTCTCTCTGCTGACGGTTGTCCGGACCCGCC
  CTGGCCTCCAGGCTCCCCTCTTGACTCTATACAGCGCCCAGGGAGTCCAGCAGCTGGGCTTGGAGCTCGG
  CCGCCCTGTCCGCTTTCTCTATGAGGACCAGAGGGGACGGCCACAAGCCTCCGCTCAGCCCATCTTCCGA
  GGCCTCAGCCTAGCAGATGGCAAATGGCACCACGTGGCTGTGGCTGTGAAGGGTCAGTCTGTCACTCTCA
  TTGTGGACTGTAAGAAGCGAGTTACCCGGCCCCTTCCCAGAAGTGTGCATCCGGTGTTGGACACCCACGG
  GGTGGTGATCTTTGGTGCCCACATCCTCGACGATGAAGTCTTTGAAGGCGATGTTCAGGAGCTCCTCGTT
  GTCCCAGGCGTCCAAGCTGCCTATCAGTCTTGTGGGCAGAAGGATCTGGAATGTGAGAGAGAACAGAGGG
  ACGGCCCTCAGACTCAGAAGCCTCACAGAGCCCAGAGATCTCCAAAGAAGGAACCAGCAAGACTTCATAA
  GCCACAGAGCCAGGAGCCCCAGAAGCAGTACCCCACCCCAGGTGAAGAGGAAGGAGTCCTGGAGTCCAGT
  CCCTTGCCATTCCTTGAAGAGGTTGCCCACGGACCCCGGGGGCTAAAGGGAGAGAAGGGAGAGCCTGCAG
  TGCTGGAGCCTGGTATGTTTGTAGAGGGACCCCCAGGCCCAGAAGGCCCAGCGGGATTAGCTGGACCCCC
  TGGCATCCAGGGGAACCCAGGCCCGGTTGGAGACCCCGGTGAGAGGGGCCCCCCTGGCCGAGCAGGGCTC
  CCCGGATCAGATGGACCCCCTGGTCCTCCCGGCACATCTCTGATGCTTCCATTCCGGTTTGGCAGTAGTG
  GGGGTGACAAGGGCCCCGTGGTGGCAGCCCAGGAGGCCCAGGCCCAGGCGATTCTGCAGCAGGCACGGCT
  GGCACTCCGTGGGCCCCCTGGCCCCATGGGTTACACGGGCCGCCCTGGACCATTGGGTCAGCCTGGGAGC
  CCTGGCTTGAAGGGAGAATCTGGAGATCTGGGCCCACAGGGCCCCAGAGGACCTCAGGGCCTCACAGGTC
  CTCCTGGCAAGGCTGGACGAAGGGGCCGAGCAGGTGCTGATGGAGCCCGTGGGATGCCGGGAGAACCTGG
  CATGAAGGGTGACCGAGGTTTCGACGGACTTCCAGGGCTACCTGGCGAGAAGGGACAAAGGGGTGATACA
  GGTGCTCAGGGCCTTCCTGGGCCTCCTGGTGAGGACGGAGAGAGGGGTGATGATGGAGAGATTGGGCCAC
  GGGGGCTGCCTGGAGAGTCGGGACCTAGAGGACTCCTTGGCCCTAAAGGCCCGCCTGGTATTCCTGGGCC
  GCCGGGAGTCCGAGGCATGGACGGTCCCCACGGCCCCAAAGGGAGCTTGGGACCTCAAGGAGAGCCAGGA
  CCTCCTGGACAACAGGGTACTCCTGGGGCCCAGGGCCTCCCCGGACCTCAGGGAGCCATCGGTCCTCATG
  GAGAGAAGGGTGCTCGTGGGAAACCAGGCCTCCCTGGCATGCCTGGATCAGATGGACTCCCGGGTCACCC
  AGGGAAGGAAGGTCCCCCTGGAACCAAAGGGAACCAGGGCCCGTCCGGACCACAGGGTCCTCTAGGATAC
  CCAGGCCCTCGAGGCGTCAAGGGTGTGGATGGAATTCGGGGCCTGAAGGGCCACAAGGGTGAAAAGGGCG
  AGGACGGCTTTCCTGGGTTCAAAGGTGACATAGGAGTGAAAGGAGACAGGGGCGAGGTTGGAGTCCCTGG
  TTCCAGGGGCGAAGACGGCCCTGAAGGGCCAAAAGGGCGCACTGGACCTACAGGAGACCCTGGACCCACT
  GGGCTCATGGGCGAGAAGGGCAAGCTAGGTGTTCCTGGTCTGCCTGGCTATCCTGGACGCCAGGGCCCCA
  AGGGATCTCTGGGTTTCCCTGGTTTTCCTGGAGCCAGTGGAGAGAAGGGAGCTCGGGGCCTGTCTGGGAA
  ATCAGGACCTCGGGGAGAACGGGGCCCCACGGGTCCAAGGGGTCAGCGGGGACCTCGAGGTGCCACTGGG
  AAATCTGGAGCTAAGGGAACATCAGGTGGTGACGGTCCCCACGGGCCACCCGGAGAGAGGGGTCTTCCTG
  GACCTCAAGGCCCCAATGGATTTCCTGGCCCCAAAGGCCCTCCGGGCCCTGCAGGGAAGGATGGGCTGCC
  GGGACACCCCGGCCAGAGAGGAGAAGTGGGATTCCAAGGAAAGACCGGCCCACCAGGCCCGCCCGGAGTG
  GTGGGACCTCAGGGAACAGCTGGAGAAAGTGGTCCCATGGGAGAGAGAGGTCACTCTGGCCCCCCAGGAC
  CTCCTGGAGAGCAAGGATTGCCTGGAACATCTGGGAAAGAAGGGACCAAGGGTGACCCTGGTCCTCCTGG
  GGCCCCAGGGAAGGATGGTCCTGCTGGTCTGAGAGGCTTCCCAGGAGAGCGAGGCCTTCCAGGCACTGCT
  GGTGGACCCGGCTTGAAAGGAAATGAAGGTCCAGCTGGCCCTCCTGGCCCTGCAGGCTCTCCTGGCGAGC
  GAGGTGCAGCAGGATCAGGGGGCCCCATTGGTCCCCCGGGACGTCCAGGCCCACAAGGTCCCCCTGGAGC
  AGCAGGAGAGAAAGGCGTACCGGGCGAGAAAGGCCCTATTGGTCCCACTGGTCGTGATGGGGTGCAGGGC
  CCCGTGGGGCTTCCTGGTCCTGCAGGACCCCCAGGCGTGGCTGGAGAGGATGGAGACAAGGGTGAAGTGG
  GAGACCCTGGACAGAAGGGAACCAAAGGAAACAAGGGTGAACATGGCCCTCCTGGACCTCCTGGTCCCAT
  CGGGCCTGTGGGGCAACCTGGAGCTGCGGGAGCTGATGGTGAGCCTGGAGCTCGGGGACCCCAGGGACAC
  TTTGGAGCCAAAGGTGATGAAGGAACAAGAGGGTTCAATGGACCCCCGGGACCCATCGGCCTACAGGGCC
  TGCCAGGACCCTCTGGGGAGAAAGGAGAAACAGGAGACGGGGGGCCTATGGGACCCCCTGGCCCTCCAGG
  ACCTCGAGGCCCCGCTGGACCCAATGGTGCTGATGGCCCACAAGGTTCCCCTGGAGGTGTTGGAAACTTG
  GGTCCCCCTGGAGAAAAGGGTGAACCGGGGGAGTCAGGGTCTCCAGGCGTCCAGGGCGAGCCGGGCGTCA
  AGGGACCACGTGGAGAGCGTGGTGAGAAAGGAGAGTCTGGGCAGGCGGGAGAGGCTGGACCACCGGGGCC
  CAAAGGCCCTACAGGCGACAATGGCCCCAAGGGGAACCCTGGTCCTGTTGGCTTTCCTGGGGACCCTGGC
  CCCCCTGGAGAAGCTGGCCCACGGGGCCAGGATGGTGCTAAGGGAGACCGAGGCGAGGATGGCGAGCCAG
  GACAACCTGGATCCCCTGGTCCCACCGGGGAGAATGGGCCCCCTGGACCCCTTGGAAAGCGGGGACCTGC
  TGGCACTCCTGGTCCAGAAGGACGGCAAGGAGAGAAGGGAGCTAAGGGGGACCCTGGTGCTGTGGGGGCC
  CCGGGAAAGACAGGCCCTGTGGGTCCTGCAGGCCTAGCAGGAAAGCCCGGCCCCGATGGTCTTCGGGGGC
  TCCCGGGTTCAGTGGGTCAGCAAGGCCGCCCTGGAGCCACAGGCCAGGCTGGGCCCCCAGGTCCTGTGGG
  ACCCCCAGGGCTTCCTGGCCTCCGGGGTGATGCTGGAGCCAAGGGGGAAAAGGGTCACCCAGGTCTCATC
  GGACTGATTGGGCCGACTGGAGAGCAAGGCGAGAAGGGCGACCGTGGCCTCCCTGGACCTCAGGGCTCAC
  CCGGACAGAAGGGAGAGACGGGTATCCCAGGAGCATCTGGCCCCATCGGTCCTGGAGGGCCTCCTGGCCT
  GCCTGGACCCTCTGGCCCCAAAGGAGCCAAAGGAGCCACAGGCCCAGCTGGACCCAAGGGAGAGAAGGGT
  GTCCAGGGCCCTCCAGGACACCCGGGCCCCCCGGGAGAGGTGATCCAGCCACTGCCCATCCAGATGCCCA
  AGAAGACCCGCCGTTCCGTGGACGGAAGCAAACTGATACAGGATGAGGAGGCTGTGCCCACTGGCGGTGC
  TCCGGGCAGTCCTGCGGGGCTGGAGGAGATCTTTGGCTCACTGGACTCTCTGCGGGAGGAGATCGAGCAG
  ATGAGGAGGCCGGCGGGGACCCAGGACAGCCCTGCTCGCACCTGCCAGGACTTGAAGCTGTGCCACCCGG
  AGCTTCCTGATGGAGAGTACTGGGTTGACCCTAACCAGGGCTGTGCTCGGGATGCCTTCCGGGTGTTCTG
  CAACTTCACAGCAGGAGGGGAGACGTGTGTCACACCCAGGGATGACGTCACACAGTTCTCCTACGTGGAC
  TCCGAGGGCTCCCCAGTGGGCGTGGTCCAGCTCACCTTCCTGCGGCTGCTCAGCGTCTCTGCCCACCAGG
  ATGTCTCCTACCCTTGCTCTGGAGTATCCCAGGATGGTCCCCTGAAACTCCGAGGGGCCAACGAGGATGA
  GCTGAGCCCTGAGACCAGCCCTTATGTCAAGGAGTTCAGAGATGGCTGTCAGACCCAGCAAGGCCGGACG
  GTGTTGGAGGTGCGCACGCCTGTACTGGAGCAGCTGCCCGTGCTGGATGCCTCCTTCGCAGACCTGGGGG
  CCCCCACAAGACGGGGAGGGGTGCTTCTGGGGCCTGTCTGCTTCATGGGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009926
• Insert Size: 4953 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009926.2, NP_034056.1
• RefSeq Size: 6346 bp
• RefSeq ORF: 4953 bp
• Locus ID: 12815
• UniProt ID: Q64739
• Cytogenetics: 17 17.98 cM
• Gene Summary: This gene encodes the alpha-2 subunit of type XI collagen, one of the low abundance fibrillar collagens found in cartilage. The encoded protein, in association with other collagen subunits, forms a heterotrimeric type XI procollagen that may undergo proteolytic processing similar to the alpha-1 subunit. Mice lacking the encoded protein exhibit a mild phenotype similar to nonocular Stickler syndrome, otospondylomegaepiphyseal dysplasia (OSMED) as well as a nonsyndromic form of deafness called DFNA13. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (2) contains one alternate in-frame exon and lacks two exons in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.