Hecw1 (NM_001081348) Mouse Untagged Clone

CAT#: MC224744

Hecw1 (untagged) - Mouse HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hecw1
• Synonyms: 9330116H24Rik; AV273951; E130207I19Rik; Nedl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224744 representing NM_001081348
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGCACCTGTGTAGTGTGAAGAATCTGTACCAGAACAGGTTTTTAGGCCTGGCTGCCATGGCGT
  CTCCATCTAGAAACTCCCAGAGCCGACGCCGGTGCAAAGAACCCCTCCGGTACAGCTATAACCCAGACCA
  ATTCCACAACATAGACATCAGAAATGGTGCCCATGATGCTATCACCATCCCTCGCTCCACCAGTGACACT
  GACCTGGTCACTTCAGACAGCCGCTCTACACTCATGGTCAGCAGTTCCTACTATTCGATAGGGCACTCCC
  AGGATTTGGTGATCCACTGGGACATCAAGGAAGAAGTGGATGCTGGAGACTGGATTGGCATGTACCTTAT
  TGGTGAAGTCTCATCTGAAAACTTTCTGGATTATAAGAACCGTGGAGTCAATGGTTCTCACAGAGGCCAG
  ATCATCTGGAAGATTGATGCCAGCTCTTACTTTGTGGAATCTGAAACTAAGATCTGCTTCAAATACTACC
  ACGGAGTAAGTGGGGCCCTGCGAGCTACAACCCCCAGTGTCACTGTCAAGAACTCAGCAGCCCCTATTTT
  TAAAGGTATTGGCTCTGAAGAGACTGCACAAAGTCAAGGAAGCCGGAGGCTGATCAGCTTCTCCCTCTCA
  GATTTCCAGGCCATGGGATTGAAGAAAGGGATGTTTTTCAACCCCGACCCTTATCTGAAGATTTCCATTC
  AACCTGGGAAACATAGCATCTTCCCAGCCCTCCCTCATCATGGACAGGAAAGGAGATCCACGATCATAGG
  CAACACTGTAAACCCCATCTGGCAGGCAGAGCATTTCAGTTTCGTGTCTCTGCCAACCGATGTGCTGGAA
  ATTGAAGTGAAGGACAAGTTTGCCAAGAGTCGTCCCATCATCAAGCGTTTCTTGGGAAAGCTGTCAATGC
  CTGTTCAGAGGCTGCTGGAGAGACACGCCATAGGGGATAGGGTGGTCAGCTACACACTTGGCCGCCGGCT
  TCCAACTGACCATGTGAGTGGGCAGCTGCAATTCCGATTTGAGATCACATCCTCCATCCATGCAGATGAT
  GAAGAGATCTCTCTGAGTGCTGAGCCTGAGTCGTCAGCAGAAACGCAGGACAGCATCATGAATAGTATGG
  TGGGAAACAGCAATGGAGAGCCTTCTGGTGATGCTACAGAATTCTGCAAGGATGCGAAGCCAGAGTCACC
  AAGTGAAGGGAATGGAGTAAACAGCTCAGAAAACCAAAACCAGGAGCATGCCGGGCCAGTTGAGGAAGCG
  GCAGGAGCTATGGAGGCCAGAGATGGTAGCAATGTCTCTGAAGCACCCGAAGAGCCCGGAGAGCTTCAGG
  ACCCAGAGCAACATGACACCCAGCCCACCCTGAGTGCAGAAGAAGTGGCCGAGGGACTTCCCCTGGATGA
  GGATTCGCCATCCTCGCTGTTGCCAGAAGAGAATACAGCTCTAGGCAGCAAGGTAGAAGAGGAAACAGTA
  CCAGAAAATGGAGCCAGAGAGGAAGAAATGCAGAAGGGGAAAGATGAAGAAGAGGAAGAAGAGGACGTTT
  CTACCCTGGAGCAAGGAGAGCCTGGACTGGAGCTGAGGGTCTCCGTGAGAAAAAAAAGCAGACCATGTTC
  CCTACCTGTATCAGAGCTTGAGACAGTGATTGCATCTGCCTGTGGAGATGCTGAGACCCCGAGAACCCAT
  TATATCCGCATCCACACCCTGCTGCACAGCATGCCCTCGGCCCAGAGAGGAAGCACCACGGAGGAGGAAG
  ATGGCTTGGAGGAGGAATCCACCCTCAAGGAGTCCTCTGAAAAGGATGGGCTCAGCGAGGTGGACACAAT
  AGCTGCTGACCCACAGTCCATGGAAGATGGCGAGAGCGATGGGGCTACTCTGTGCATGGCACCCTCTGAC
  TGCTCTGGGGGACATTTCTCCAGTTTATCCAAAGGCATTGGTGCTGGCCAAGATGGCGAAGCTCACCCCA
  GCACTGGGAGTGAGAGTGACTCCAGCCCCCAGCAGGGAGCAGACCACAGTTGCGAGGGCTGTGATGCCTC
  ATGCTGCAGCCCGTCTTGCTATAGCACATCTTGCTACAGCAGCTCTTGTTACAGCAGCTCCTGTTACAGC
  AGCTCCTGCTACAATGGTAACAATCGGTTCGCCAGCCACACACGCTTCTCCTCTGTGGACAGCGCCAAGA
  TCTCTGAGAGCACAGTCTTTTCTTCACAAGAGGATGAGGAAGAGGAAAACAGTGCATTCGAGTCGGTACC
  CGACTCGGTGCAAAGCCCAGAGCTAGATCCCGAGTCTACAAATGGGGCTGGCCCTTGGCAGGATGAACTG
  GCTGCCCCTGGTGGGAATGCAGCAAGAAGTACTGAAGGTCTGGAGTCCCCCATGGCAGGTCCTAGCAATA
  GGAGAGAAGGTGAATGTCCTATACTCCATAATTCCCAGCCAATAAGCCAACTTCCTTCCTTGAGGCCTGA
  ACATCATCACTACCCAACAATCGATGAGCCTCTTCCACCAAACTGGGAGGCTCGAATTGACAGCCATGGA
  CGGGTCTTTTATGTGGACCATATAAATCGCACAACCACCTGGCAGCGACCATCGATGGCCCCTACCCCCG
  ATGGAATGATCAGATCCGGGTCTGTCCACCAGATGGAGCAGCTTAACAGAAGGTATCAAAACATCCAGAG
  GACCATGGCAACAGAGAGAGCTGAAGAAGATTCTGGCAATCAAAACTCTGAGCAAATACCAGACGGAGGA
  GGAGGAGGTGGTGGTGGGAGTGACTCGGAAGCAGAATCATCCCAATCAAGCTTAGATCTGAGGAGAGAAG
  GGTCACTTTCTCCTGTCAACTCACAGAAGGTCACCTTGCTGCTGCAGTCTCCAGCGGTCAAGTTCATCAC
  CAATCCTGAGTTCTTCACTGTGTTGCATGCCAATTATAGTGCCTACAGGGTTTTCACAAGCAGCACTTGC
  CTGAAGCATATGATTTTAAAAGTCCGAAGAGATGCTCGAAATTTTGAGCGCTATCAGCACAACCGGGACC
  TGGTGAATTTCATCAACATGTTTGCAGATACTCGGCTGGAACTGCCCCGGGGTTGGGAGATCAAAACTGA
  TCATCAGGGAAAGTCTTTCTTCGTGGACCACAACAGTCGAGCTACTACTTTCATAGATCCCCGAATCCCT
  CTTCAGAATGGCCGTCTTCCCAATCATCTAACCCACCGACAGCACCTCCAGAGGCTCCGGAGTTACAGTG
  CTGGGGAGGCCTCAGAAGTCTCTAGAAACAGAGGAGCATCTTTGCTGGCCAGACCAGGACACAGCCTAAT
  AGCTGCTATTCGAAGCCAACATCAACATGAATCCTTGCCACTGGCATACAATGACAAGATTGTGGCATTT
  CTTCGCCAGCCAAACATTTTTGAAATGCTACAAGAACGTCAGCCAAGCTTGGCAAGAAACCACACACTCA
  GGGAGAAAATCCATTATATTCGGACAGAGGGCAATCATGGGCTTGATAAATTGTCCTGTGATGCAGATCT
  AGTCATTTTGCTGAGTCTCTTTGAAGAAGAGATCATGTCCTATGTTCCCCTGCAATCTGCCTTCCACCCT
  GGATATAGCTTCTCTCCCCGCTGTTCACCCTGTTCCTCACCTCAGAACTCCCCAGGTTTACAGAGAGCCA
  GCGCAAGAGCCCCTTCACCATACCGAAGGGATTTTGAAGCTAAGCTCCGCAATTTCTACCGAAAACTGGA
  AGCCAAAGGATTTGGACAGGGTCCGGGTAAAATTAAGCTCATCATCCGTCGAGACCACTTGTTGGAGGGG
  ACCTTCAATCAAGTGATGGCATACTCAAGGAAGGAGCTCCAGAGAAACAAGCTCTACATCACCTTTGTTG
  GAGAGGAGGGGCTGGACTACAGTGGCCCTTCTCGAGAGTTCTTCTTCCTGTTGTCTCAGGAGCTCTTCAA
  CCCTTACTATGGACTCTTTGAGTATTCTGCAAATGACACATACACAGTACAGATCAGCCCCATGTCAGCA
  TTTGTAGAAAACTATCTTGAATGGTTTAGATTCAGTGGTCGTATCCTGGGCCTAGCTCTGATCCATCAGT
  ACCTCCTGGACGCTTTCTTCACAAGACCCTTCTATAAGGGACTCCTGAAGCTGCCATGTGATCTGAGTGA
  CCTAGAATATTTGGATGAGGAATTCCACCAGAGTTTACAGTGGATGAAGGACAACAACATCACAGACATT
  CTAGATCTCACCTTCACTGTTAATGAAGAAGTTTTTGGACAGGTAACAGAAAGAGAGTTGAAGTCTGGAG
  GCGCCAACACGCAGGTGACTGAGAAAAACAAGAAGGAATACATAGAGAGGATGGTGAAGTGGCGTGTGGA
  GCGTGGTGTGGTACAGCAGACGGAGGCATTGCTCAGGGGTTTCTATGAGGTTGTAGACTCAAGGCTCGTC
  TCAGTGTTTGATGCGAGGGAACTGGAGCTGGTGATCGCTGGAACTGCTGAAATCGACCTGAATGACTGGA
  GGAATAACACAGAGTACCGGGGAGGTTATCACGATGGCCACCTTGTGATCCGCTGGTTCTGGGCTGCAGT
  GGAACGCTTCAATAATGAGCAGAGATTGCGATTACTTCAGTTTGTGACAGGAACATCAAGTGTGCCCTAT
  GAAGGCTTTGCAGCTCTGCGGGGAAGCAATGGGCTTCGACGTTTCTGCATAGAAAAGTGGGGGAAGATTA
  CATCTCTTCCCAGGGCACATACGTGCTTCAACCGATTGGATCTCCCACCATACCCTTCATATTCCATGTT
  GTATGAAAAGCTGTTAACAGCAGTAGAAGAAACCAGCACCTTTGGACTTGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081348
• Insert Size: 4815 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081348.3, NP_001074817.3
• RefSeq Size: 9460 bp
• RefSeq ORF: 4815 bp
• Locus ID: 94253
• UniProt ID: Q8K4P8
• Cytogenetics: 13 5.29 cM
• Gene Summary: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent degradation of DVL1.[UniProtKB/Swiss-Prot Function]