Adamts12 (NM_175501) Mouse Untagged Clone

CAT#: MC224741

Adamts12 (untagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 12 (Adamts12), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adamts12
• Synonyms: ADAMTS-12; AI605170
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224741 representing NM_175501
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCATGTGCTCGGGGGAGCTGGCTGGCCAAGCTCTCGATCGTGGCTCAGCTTATTAATTTTGGGGCTT
  TTTGCCACGGGAGACAAACTCAGCCCTGGCCGGTTCGCTTCCCAGACCCGAGGCAAGAACATTTTATCAA
  GTCCCTGCCAGAATACCACATAGTGAGTCCAGTCCAAGTCGATGCTGGTGGCCATGTTCTGTCCTATGGC
  CTGCATCACCCTGTCACTAGCAGCAGGAAGAAGAGAGCTGCAGGTGGTTCTGGGGACCAGCTGTACTACA
  GAATTTCGCATGAAGAAAAAGACCTGTTTTTCAATCTGACGGTCAACTGGGAATTTCTTTCCAACGGCTA
  TGTCGTGGAGAAGAGATATGGGAACCTTTCCCATGTTAAGATGGTGGCTTCCTCAGGCCAGCCCTGCCAT
  CTCAGGGGCACAGTTCTGCAGCAGGGCACCACAGTCGGGATCGGGACAGCAGCTCTCAGTGCCTGCCAAG
  GACTGACTGGATTTTTCCATCTACCACATGGAGACTTTTTCATCGAGCCTGTTAAAAAGCATCCACTGAC
  CGAGGAAGGGTCCTACCCTCATGTCGTATACAGGAGACAGAGCATCAGAGCTCCAGAGACAAAGGAGCCC
  ATCTGTGGATTAAAGGACAGTCTTGATAACTCTGTGAAACAAGAGCTACAGCGGGAGAAGTGGGAGAGGA
  AAACCTTGCGGAGCAGAAGCCTCTCCCGACGTTCCATAAGCAAGGAGAGATGGGTGGAGACCCTCGTGGT
  GGCCGACACGAAGACGGTAGAGTACCATGGAAGCGAGAACGTGGAGTCGTACATCCTCACCATCATGAAT
  ATGGTCACTGGGTTGTTTCATAGCCCAAGCATTGGCAATCTAGTCCACATTGTTGTGGTTCGGCTCATTC
  TACTTGAAGAAGAGGAGCAAGGATTGAAAATAGTTCATCATGCAGAGAAGACACTGTCCAGTTTCTGCAA
  GTGGCAGAAGAGCATCAATCCCAAGAGTGACCTCAACCCTGTCCATCATGATGTGGCTGTCCTTATCACC
  AGAAAGGACATCTGTGCTGGTGTCAATCGCCCTTGTGAGACTCTGGGGCTGTCCCAATTGTCAGGAATGT
  GCCAGCCTCACAGGAGCTGTAACATCAATGAAGACTCTGGCCTCCCCCTGGCTTTCACCATTGCACATGA
  ACTTGGTCACAGCTTCGGCATCCAGCATGATGGAAAGGAAAATGACTGTGAGCCCGTGGGCAGGCACCCA
  TACATCATGTCCCAACAGATTCAGTACGACCCAACTCCACTAACATGGTCGAAATGCAGCAAGGAGTACA
  TCACTCGCTTCTTGGACCGAGGCAGGGGGTTCTGTCTTGATGATATCCCCAGCAAGAAAGGCTTGAAGTC
  CAACGTCATTGCTCCTGGAGTAATTTACGATGTTCATCACCAGTGCCAGCTCCAATATGGCCCAAATGCT
  ACATTCTGCCAGGAAGTGGAAAATGTTTGCCAGACACTGTGGTGCTCGGTGAAAGGCTTCTGTCGCTCTA
  AGCTAGATGCTGCTGCAGACGGGACTCGTTGCGGTGAGAAGAAGTGGTGTATGGCTGGAAAGTGTATCAC
  AGTGGGGAAGAAACCAGAGAGCATCCCTGGAGGCTGGGGACGCTGGTCACCCTGGTCACACTGTTCTAGA
  ACATGTGGGGCAGGAGCTCAGAGCGCAGAGAGGCTCTGCAACAACCCAGAACCAAAATTTGGAGGGAAAT
  ACTGCACCGGAGAAAGAAAACGCTATCGCTTATGCAATGTCCACCCCTGCCGCTCAGACACTCCAACTTT
  CCGGCAGATGCAGTGCAGTGAATTTGACACAGTCCCCTACAAAAACCAATTCTACCGCTGGTTCCCAGTG
  TTTAATGCAGCACATCCTTGTGAGCTGTACTGCAGACCCATAGATGAACAGTTTTCTGAGAGAATGCTGG
  AAGCTGTCATTGATGGCACCCCATGCTTCGAAGGTGGCAACAGCAGAAATGTCTGTATTAATGGCATATG
  TAAGAGGGTTGGCTGTGACTATGAGATTGATTCCAATGCTACAGAGGATCGCTGTGGTGTGTGCCTTGGA
  GATGGCTCTGCCTGCCAGACGGTGAAGAAGCTGTTTAGACAGAAGGAAGGATCTGGTTACGTTGACATTG
  GACTTATTCCCAAAGGTGCGAGGGATATAAGGGTGATGGAAATCAAGGCAGCTGGCAATTTCCTGGCCAT
  TAGGAGTGAAGATCCGGAAAAATACTACCTCAACGGAGGGTTCATTATCCAGTGGAATGGAAACTATAAA
  CTGGCAGGGACCGTTTTCCAGTATGACAGGAAAGGAGATCTGGAGAAACTTATAGCTCCAGGCCCCACCA
  ACGAGTCAGTGTGGCTCCAGCTACTTTTCCAGGTGACTAACCCTGGTATCAAGTATGAGTACACAGTCCG
  GAAAGATGGCCTTGACAATGACGTGGAGAAGTTGCTGTACTTCTGGCAATTTGGCCGCTGGACAGAATGC
  AGTGTAACGTGTGGAACAGGTATCCGCCGGCAGGCTGCTCATTGTGTCAAGAAGGGCCACGGTATAGTGA
  AAACGACCTTCTGTAACCCAGAAACACAGCCCAGCGTGAGACAGAAGAAGTGCCATGAAAAGGACTGTCC
  ACCCAGGTGGTGGGCAGGGGAGTGGGAAGCGTGTTCAACGACATGTGGGCCCTACGGAGAAAAAAAGAGA
  ACGGTGCTGTGCATCCAGACCATGGGCTCGGATGAACAGGCTCTCCCTGCTACAGACTGCCAGCACCTGC
  TGAAGCCCAAAGCACTGGTTTCCTGCAACAGAGATATTCTGTGTCCATCAGACTGGACAGTAGGCAACTG
  GAGTGAGTGCTCTGTTTCCTGTGGTGGTGGAGTGCGGATTCGCAGTGTCACATGTGCCAAGAACCTTAAT
  GAGCCTTGTGACAAGACCCGGAAACCCAACAGTCGAGCTCTGTGCGGCCTCCAGCAGTGCCCATTTAGCC
  GTAGAGTTCTGAAACCCAATAAAGATATAGCTCCCAGTGGGAAAAATCAATCGACAGCAGAACATGACCC
  CTTCAAGCCCATCCCAGCACCAACATCCAGGCCCACGCCACTGTCCACACCCACAGTGCCTGAGTCTATG
  AGCACAAGTACTCCCACAATCAACAGCCTTGGCTCCACCATAGCATCCCAAGAAGACGCAAATGGGATGG
  GATGGCAGAATAATTCAACCCAAGCTGAAGAGGGCTCCCACTTCCCTACTTCTTCTGGAAGCACTTCCCA
  GGTCCCCGTGACTTCCTGGTCTTTGAGTATCCAACCTGATGATGAGAATGTTTCCAGCTCAGCTATAGGT
  CCTACTTCAGAGGGTGACTTTTGGGCAACCACAACAAGTGATTCTGGCTTATCATCTAGCGATGCTATGA
  CTTGGCAGGTAACTCCATTTTATAGCACCATGACCACAGATCCAGAAGTGGAGATACATAGTGGTTCAGG
  GGAAGACAGTGACCAGCCTTTGAACAAGGACAAAAGCAACTCTGTGATATGGAATAAGATCGGGGTACCT
  GAACATGATGCTCCCATGGAGACGGATGCAGAACTACCACTTGGACCTCCACCAACATCTTATATGGGGG
  AAGAGCCCTCATGGCCTCCCTTCAGTACAAAAATGGAAGGCTCATTACCTGCCTGGTCCTTCAAAAATGA
  GACACCCAGGGATGATGGGATGATCGCTGAAAAATCAAGGAAGATTCCACTCCCTCTTGCAGGAGACCAC
  CATCCAGCAACCTCAGAGAAGTTAGAAAACCATGACAAATTGGCATTGCCAAATACTACAAATCCAACCC
  AAGGCTTTGGGCCTGTCCTGACTGAAGAAGATGCCTCCAATCTGATTGCCGAGGGGTTTTTGCTAAATGC
  TTCTGATTACAAGCATCTTATGAAGGACCACAGCCCTGCATACTGGATTGTTGGAAACTGGAGTAAATGC
  TCCACTACCTGTGGGCTGGGGGCCTACTGGAGGAGTGTGGAGTGCAGCTCTGGGGTGGACGCTGACTGCA
  CAACCATTCAGAGGCCAGACCCTGCTAAGAAGTGCCACCTCCGCCCCTGTGCTGGCTGGCGAGTGGGAAA
  CTGGAGCAAGTGCTCCCGAAATTGCAGTGGAGGCTTCAAGATTCGTGAGGTTCAGTGCATGGACAGCCTG
  GACCACCACCGGAGCCTGAGGCCGTTTCACTGTCAATTCCTAGCTGGCGCTCCTCCTCCACTGAGTATGA
  GCTGTAACCTGGAGCCTTGTGGAGAATGGCAAGTGGAGCCCTGGAGCCAGTGTTCTAGGTCCTGTGGAGG
  AGGTGTTCAGGAGAGAGGAGTGTCTTGTCCTGGAGGCCTCTGTGACTGGACAAAAAGGCCTGCAACCACT
  GTGCCCTGCAACAGACACCTTTGCTGTCACTGGGCCACTGGGAACTGGGAGCTGTGTAACACTTCCTGTG
  GTGGTGGCTCTCAGAAAAGAACCATTCATTGCATCCCCTCAGAAAACAGTACAACTGAAGATCAAGACCA
  GTGCCTCTGTGACCATCAAGTCAAACCCCCAGAATTCCAAACATGCAACCAGCAAGCCTGCAGGAAGAGT
  GCTGATTTAACTTGCTTGAAGGACAGACTTTCAATCAGCTTCTGCCAGACACTGAAATCCATGAGGAAGT
  GCTCTGTGCCATCAGTGCGAGCCCAGTGCTGTCTCTCGTGTCCACAGGCGCCCAGCATCCACACCCAGAG
  GCAAAGAAAACAGCAGTTGCTCCAAAATCATGACATGCTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175501
• Insert Size: 4803 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175501.2, NP_780710.2
• RefSeq Size: 6234 bp
• RefSeq ORF: 4803 bp
• Locus ID: 239337
• UniProt ID: Q811B3
• Cytogenetics: 15 A1
• Gene Summary: This gene encodes a member of "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. The encoded preproprotein undergoes proteolytic processing to generate an active protease. Mice lacking the encoded protein exhibit increased angiogenic response and tumor invasion in different models of angiogenesis and, severe inflammation and delayed recovery when subjected to experimental conditions that induce colitis, endotoxic sepsis and pancreatitis. [provided by RefSeq, Jul 2016]